<i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup>
Lipid profile comparative analysis was performed to reveal the interdependence of lipids with <i>Circadian locomoter output cycles protein kaput</i> (<i>CLOCK</i>) 3111T/C gene polymorphism in menopausal women with/without a body mass index (BMI) of ≥25 kg/m<sup>2</s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Pathophysiology |
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| Online Access: | https://www.mdpi.com/1873-149X/28/1/1 |
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| author | Natalya Semenova Irina Madaeva Sergey Kolesnikov Lyubov Rychkova Tatjana Bairova Marina Darenskaya Lyubov Kolesnikova |
| author_facet | Natalya Semenova Irina Madaeva Sergey Kolesnikov Lyubov Rychkova Tatjana Bairova Marina Darenskaya Lyubov Kolesnikova |
| author_sort | Natalya Semenova |
| collection | DOAJ |
| description | Lipid profile comparative analysis was performed to reveal the interdependence of lipids with <i>Circadian locomoter output cycles protein kaput</i> (<i>CLOCK</i>) 3111T/C gene polymorphism in menopausal women with/without a body mass index (BMI) of ≥25 kg/m<sup>2</sup>. Methods: A total of 193 female volunteers aged 45 to 60 years were divided into two groups: Those with BMI < 25 kg/m<sup>2</sup> (control) and those with BMI ≥ 25 kg/m<sup>2</sup>. Each group was then divided into two subgroups: Those with the <i>CLOCK TT</i>-genotype and those with the <i>CLOCK TC-</i>, <i>CC</i>-genotypes. Lipid metabolism parameters were determined by the enzymatic method. Single-nucleotide polymorphisms (SNPs) were detected via polymerase chain reaction–restriction fragment length polymorphism technology. Results: There were no differences in <i>CLOCK 3111T/C</i> genotypes or allele frequency between the control and main groups. In addition, there were no differences in lipid profile parameters between women of the control group and different <i>CLOCK 3111T/C</i> genotypes. The total cholesterol (<i>p</i> = 0.041) and low-density lipoprotein cholesterol (<i>p</i> = 0.036) levels were higher in the subgroup of women with a BMI ≥ 25 kg/m<sup>2</sup> and <i>CLOCK TT</i>-genotype as compared to the subgroup with a BMI ≥ 25 kg/m<sup>2</sup> and minor allele <i>3111C.</i> Conclusions: SNP <i>3111T/C</i> of the <i>CLOCK</i> gene is not associated with BMI however, data suggest that the minor allele of the <i>CLOCK 3111T/C</i> gene polymorphism may have a protective role in atherogenic lipid levels in women with a BMI greater than or equal to 25 kg/m<sup>2</sup>. |
| first_indexed | 2024-04-11T11:23:38Z |
| format | Article |
| id | doaj.art-509571ebf4e841339a84e557c0ad3a18 |
| institution | Directory Open Access Journal |
| issn | 1873-149X |
| language | English |
| last_indexed | 2024-04-11T11:23:38Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathophysiology |
| spelling | doaj.art-509571ebf4e841339a84e557c0ad3a182022-12-22T04:26:23ZengMDPI AGPathophysiology1873-149X2020-12-012811910.3390/pathophysiology28010001<i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup>Natalya Semenova0Irina Madaeva1Sergey Kolesnikov2Lyubov Rychkova3Tatjana Bairova4Marina Darenskaya5Lyubov Kolesnikova6Scientific Centre for Family Health and Human Reproduction Problems, 664003 Irkutsk, RussiaScientific Centre for Family Health and Human Reproduction Problems, 664003 Irkutsk, RussiaScientific Centre for Family Health and Human Reproduction Problems, 664003 Irkutsk, RussiaScientific Centre for Family Health and Human Reproduction Problems, 664003 Irkutsk, RussiaScientific Centre for Family Health and Human Reproduction Problems, 664003 Irkutsk, RussiaScientific Centre for Family Health and Human Reproduction Problems, 664003 Irkutsk, RussiaScientific Centre for Family Health and Human Reproduction Problems, 664003 Irkutsk, RussiaLipid profile comparative analysis was performed to reveal the interdependence of lipids with <i>Circadian locomoter output cycles protein kaput</i> (<i>CLOCK</i>) 3111T/C gene polymorphism in menopausal women with/without a body mass index (BMI) of ≥25 kg/m<sup>2</sup>. Methods: A total of 193 female volunteers aged 45 to 60 years were divided into two groups: Those with BMI < 25 kg/m<sup>2</sup> (control) and those with BMI ≥ 25 kg/m<sup>2</sup>. Each group was then divided into two subgroups: Those with the <i>CLOCK TT</i>-genotype and those with the <i>CLOCK TC-</i>, <i>CC</i>-genotypes. Lipid metabolism parameters were determined by the enzymatic method. Single-nucleotide polymorphisms (SNPs) were detected via polymerase chain reaction–restriction fragment length polymorphism technology. Results: There were no differences in <i>CLOCK 3111T/C</i> genotypes or allele frequency between the control and main groups. In addition, there were no differences in lipid profile parameters between women of the control group and different <i>CLOCK 3111T/C</i> genotypes. The total cholesterol (<i>p</i> = 0.041) and low-density lipoprotein cholesterol (<i>p</i> = 0.036) levels were higher in the subgroup of women with a BMI ≥ 25 kg/m<sup>2</sup> and <i>CLOCK TT</i>-genotype as compared to the subgroup with a BMI ≥ 25 kg/m<sup>2</sup> and minor allele <i>3111C.</i> Conclusions: SNP <i>3111T/C</i> of the <i>CLOCK</i> gene is not associated with BMI however, data suggest that the minor allele of the <i>CLOCK 3111T/C</i> gene polymorphism may have a protective role in atherogenic lipid levels in women with a BMI greater than or equal to 25 kg/m<sup>2</sup>.https://www.mdpi.com/1873-149X/28/1/1<i>CLOCK</i> 3111T/C polymorphismatherogenic lipidsbody mass index |
| spellingShingle | Natalya Semenova Irina Madaeva Sergey Kolesnikov Lyubov Rychkova Tatjana Bairova Marina Darenskaya Lyubov Kolesnikova <i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup> Pathophysiology <i>CLOCK</i> 3111T/C polymorphism atherogenic lipids body mass index |
| title | <i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup> |
| title_full | <i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup> |
| title_fullStr | <i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup> |
| title_full_unstemmed | <i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup> |
| title_short | <i>CLOCK 3111TT</i> Genotype Is Associated with Increased Total Cholesterol and Low-Density Lipoprotein Levels in Menopausal Women with a Body Mass Index of at Least 25 kg/m<sup>2</sup> |
| title_sort | i clock 3111tt i genotype is associated with increased total cholesterol and low density lipoprotein levels in menopausal women with a body mass index of at least 25 kg m sup 2 sup |
| topic | <i>CLOCK</i> 3111T/C polymorphism atherogenic lipids body mass index |
| url | https://www.mdpi.com/1873-149X/28/1/1 |
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