<i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation
Polysaccharides have attracted much attention in the food industry due to their diverse biological activities. To date, research on the mechanism of polysaccharide synthesis has mainly focused on the role of crucial enzymes in the polysaccharide synthesis pathway, but other genes that regulate polys...
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MDPI AG
2022-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/9/949 |
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| author | Zi Wang Ju-Hong Chen Ling-Shuai Wang Juan Ding Ming-Wen Zhao Rui Liu |
| author_facet | Zi Wang Ju-Hong Chen Ling-Shuai Wang Juan Ding Ming-Wen Zhao Rui Liu |
| author_sort | Zi Wang |
| collection | DOAJ |
| description | Polysaccharides have attracted much attention in the food industry due to their diverse biological activities. To date, research on the mechanism of polysaccharide synthesis has mainly focused on the role of crucial enzymes in the polysaccharide synthesis pathway, but other genes that regulate polysaccharide synthesis have not been well explored. In this study, the <i>GlPP2C1</i> gene, encoding a phosphoprotein type 2C phosphatase, was cloned, and PP2C-silenced strains (PP2C1i-1 and PP2C1i-3) were screened. Measurements of the polysaccharide content and cell wall tolerance revealed that <i>GlPP2C1</i> silencing increased the polysaccharide content and enhanced cell wall resistance in <i>Ganoderma lingzhi.</i> The contents of intracellular polysaccharides (IPS), extracellular polysaccharides (EPS) and β-1,3-D-glucan in PP2C-silenced strains were increased by 25%, 33% and 36%, respectively, compared with those in the WT strains and strains transformed with an empty vector. Further mechanistic studies showed that <i>GlPP2C1</i> silencing increased the content of <i>Ganoderma lingzhi</i> polysaccharides (GL-PS) through Slt2. In summary, this study revealed the mechanism through which protein phosphatase regulates GL-PS biosynthesis for the first time. |
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| language | English |
| last_indexed | 2024-03-09T23:30:07Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-50981c314eb54f99b1f609bc9b432c8b2023-11-23T17:09:45ZengMDPI AGJournal of Fungi2309-608X2022-09-018994910.3390/jof8090949<i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 PhosphorylationZi Wang0Ju-Hong Chen1Ling-Shuai Wang2Juan Ding3Ming-Wen Zhao4Rui Liu5Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaPolysaccharides have attracted much attention in the food industry due to their diverse biological activities. To date, research on the mechanism of polysaccharide synthesis has mainly focused on the role of crucial enzymes in the polysaccharide synthesis pathway, but other genes that regulate polysaccharide synthesis have not been well explored. In this study, the <i>GlPP2C1</i> gene, encoding a phosphoprotein type 2C phosphatase, was cloned, and PP2C-silenced strains (PP2C1i-1 and PP2C1i-3) were screened. Measurements of the polysaccharide content and cell wall tolerance revealed that <i>GlPP2C1</i> silencing increased the polysaccharide content and enhanced cell wall resistance in <i>Ganoderma lingzhi.</i> The contents of intracellular polysaccharides (IPS), extracellular polysaccharides (EPS) and β-1,3-D-glucan in PP2C-silenced strains were increased by 25%, 33% and 36%, respectively, compared with those in the WT strains and strains transformed with an empty vector. Further mechanistic studies showed that <i>GlPP2C1</i> silencing increased the content of <i>Ganoderma lingzhi</i> polysaccharides (GL-PS) through Slt2. In summary, this study revealed the mechanism through which protein phosphatase regulates GL-PS biosynthesis for the first time.https://www.mdpi.com/2309-608X/8/9/949PP2C<i>Ganoderma lingzhi</i>GL-PSSlt2 |
| spellingShingle | Zi Wang Ju-Hong Chen Ling-Shuai Wang Juan Ding Ming-Wen Zhao Rui Liu <i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation Journal of Fungi PP2C <i>Ganoderma lingzhi</i> GL-PS Slt2 |
| title | <i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation |
| title_full | <i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation |
| title_fullStr | <i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation |
| title_full_unstemmed | <i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation |
| title_short | <i>GlPP2C1</i> Silencing Increases the Content of <i>Ganoderma</i><i>lingzhi</i> Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation |
| title_sort | i glpp2c1 i silencing increases the content of i ganoderma i i lingzhi i polysaccharide gl ps and enhances slt2 phosphorylation |
| topic | PP2C <i>Ganoderma lingzhi</i> GL-PS Slt2 |
| url | https://www.mdpi.com/2309-608X/8/9/949 |
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