Byproduct from Triphala Extraction as Tannin and Rutin Sources for Production of Gallic Acid, Isoquercetin and Quercetin by Solid-State Fermentation
Byproduct from Triphala extraction process (BTP) was studied as a substrate for gallic acid, isoquercetin and quercetin production by Aspergillus niger fermentation in this research. The results showed that BTP was a good source of tannin and rutin. Nevertheless, the activity of A. niger on BTP as a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Croatian Society of Chemical Engineers
2022-07-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/06-2-2022.pdf |
| Summary: | Byproduct from Triphala extraction process (BTP) was studied as a substrate for gallic acid, isoquercetin and quercetin production by Aspergillus niger fermentation in this research. The results showed that BTP was a good source of tannin and rutin. Nevertheless, the activity of A. niger on BTP as a sole substrate was very low. Supplementing nitrogen sources was found to be a key to enhancing conversion of tannin to gallic acid, and rutin to isoquercetin or isoquercetin and quercetin. BTP with 0.75 % sodium nitrate was suggested to be an optimal supplemented nitrogen source for the production of gallic acid and isoquercetin in this research, which yielded the highest contents of 61.6±2.16 mg g–1 DS and 3.27±0.29 mg g–1 DS, respectively. In addition, the highest extraction yields of gallic acid, isoquercetin and quercetin were obtained by an ultrasound-assisted extraction using methanol as an extraction solvent as 12.24±2.12 mg g–1 DS which was around 0.5 time higher than the one without ultrasound-assisted extraction (8.84±1.12 mg g–1 DS). |
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| ISSN: | 0352-9568 1846-5153 |