α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MS
Objective: To identify the compounds with α-glucosidase inhibitory activity from Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹) using HPLC-ESI-QTOF-MS/MS. Methods: The ethanol extracts of Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹) were partitioned with petroleum ether, ethyl acetate, n-butanol, and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2019-03-01
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| Series: | Digital Chinese Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589377719300801 |
| _version_ | 1818518754046246912 |
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| author | Huang Xiao-Long Wan Dan Shu Jun Fang Liang-Zi Zou Du-Zhun Zhang Shui-Han |
| author_facet | Huang Xiao-Long Wan Dan Shu Jun Fang Liang-Zi Zou Du-Zhun Zhang Shui-Han |
| author_sort | Huang Xiao-Long |
| collection | DOAJ |
| description | Objective: To identify the compounds with α-glucosidase inhibitory activity from Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹) using HPLC-ESI-QTOF-MS/MS. Methods: The ethanol extracts of Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹) were partitioned with petroleum ether, ethyl acetate, n-butanol, and water. The assay for α-glucosidase inhibitory activity revealed strong α-glucosidase inhibitory activity in the ethyl acetate fraction, and the bioactive compounds present in this fraction were identified by the HPLC- ESI-QTOF-MS/MS method. Results: A total of 29 compounds were determined, among the identified bioactive components; these included 12 phenylethanoid glycosides (compounds 5, 6, 12-17, 20-22, 24), 7 flavonoids (compounds 10, 19, 23, 25-28), 5 phenolic acids (compounds 2-4, 7, 9), and 5 other compounds. Compounds 2-4, 7, 9-10, 12-13, 15, 19, and 26, with a potential α-glucosidase inhibitory activity, have been reported previously. Conclusions: Our results show that the methodology used in this study is feasible, credible, and rapid in identifying known compounds and also for characterizing new natural α- glucosidase inhibitory candidates from Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹). Keywords: α-glucosidase inhibitor, Clerodendrum bungei Steud (Chou Mu Dan, 臭, 牡,丹), HPLC-ESI-QTOF-MS/MS |
| first_indexed | 2024-12-11T01:14:33Z |
| format | Article |
| id | doaj.art-50a194a59ce449babccfa056792fd349 |
| institution | Directory Open Access Journal |
| issn | 2589-3777 |
| language | English |
| last_indexed | 2024-12-11T01:14:33Z |
| publishDate | 2019-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Digital Chinese Medicine |
| spelling | doaj.art-50a194a59ce449babccfa056792fd3492022-12-22T01:25:56ZengKeAi Communications Co., Ltd.Digital Chinese Medicine2589-37772019-03-01214149α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MSHuang Xiao-Long0Wan Dan1Shu Jun2Fang Liang-Zi3Zou Du-Zhun4Zhang Shui-Han5Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China; Research Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha, Hunan, 410006, ChinaResearch Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha, Hunan, 410006, ChinaHunan University of Chinese Medicine, Changsha, Hunan, 410208, China; Research Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha, Hunan, 410006, ChinaHunan University of Chinese Medicine, Changsha, Hunan, 410208, ChinaHunan University of Chinese Medicine, Changsha, Hunan, 410208, China; Research Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha, Hunan, 410006, ChinaResearch Institute of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha, Hunan, 410006, China; Corresponding author. Researcher. Research direction: preparation and quality standard of traditional Chinese medicine.Objective: To identify the compounds with α-glucosidase inhibitory activity from Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹) using HPLC-ESI-QTOF-MS/MS. Methods: The ethanol extracts of Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹) were partitioned with petroleum ether, ethyl acetate, n-butanol, and water. The assay for α-glucosidase inhibitory activity revealed strong α-glucosidase inhibitory activity in the ethyl acetate fraction, and the bioactive compounds present in this fraction were identified by the HPLC- ESI-QTOF-MS/MS method. Results: A total of 29 compounds were determined, among the identified bioactive components; these included 12 phenylethanoid glycosides (compounds 5, 6, 12-17, 20-22, 24), 7 flavonoids (compounds 10, 19, 23, 25-28), 5 phenolic acids (compounds 2-4, 7, 9), and 5 other compounds. Compounds 2-4, 7, 9-10, 12-13, 15, 19, and 26, with a potential α-glucosidase inhibitory activity, have been reported previously. Conclusions: Our results show that the methodology used in this study is feasible, credible, and rapid in identifying known compounds and also for characterizing new natural α- glucosidase inhibitory candidates from Clerodendrum bungei Steud (Chou Mu Dan, 臭牡丹). Keywords: α-glucosidase inhibitor, Clerodendrum bungei Steud (Chou Mu Dan, 臭, 牡,丹), HPLC-ESI-QTOF-MS/MShttp://www.sciencedirect.com/science/article/pii/S2589377719300801 |
| spellingShingle | Huang Xiao-Long Wan Dan Shu Jun Fang Liang-Zi Zou Du-Zhun Zhang Shui-Han α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MS Digital Chinese Medicine |
| title | α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MS |
| title_full | α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MS |
| title_fullStr | α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MS |
| title_full_unstemmed | α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MS |
| title_short | α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI- QTOF-MS/MS |
| title_sort | α glucosidase inhibitory activity guided identification of compounds from clerodendrum bungei steud by hplc esi qtof ms ms |
| url | http://www.sciencedirect.com/science/article/pii/S2589377719300801 |
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