Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries
Mastoidectomy is a surgical procedure for the treatment of chronic otitis media. This study investigated the ability of rat stromal vascular fraction cells (rSVF) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation-enhancing blood products to promote the regeneration...
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MDPI AG
2022-02-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/5/877 |
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| author | Sung-Hee Park Hantai Kim Yun Yeong Lee Yeon Ju Kim Jeong Hun Jang Oak-Sung Choo Yun-Hoon Choung |
| author_facet | Sung-Hee Park Hantai Kim Yun Yeong Lee Yeon Ju Kim Jeong Hun Jang Oak-Sung Choo Yun-Hoon Choung |
| author_sort | Sung-Hee Park |
| collection | DOAJ |
| description | Mastoidectomy is a surgical procedure for the treatment of chronic otitis media. This study investigated the ability of rat stromal vascular fraction cells (rSVF) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation-enhancing blood products to promote the regeneration of mastoid bone defect. Twenty male Sprague Dawley rats were randomly divided according to obliteration materials: (1) control, (2) PCL scaffold only, (3) rSVFs + PCL, (4) rSVFs + PCL + platelet-rich plasma, and (5) rSVFs + PCL + whole plasma (WP). At 7 months after transplantation, the rSVFs + PCL + WP group showed remarkable new bone formation in the mastoid. These results indicate that SVFs, PCL scaffolds, and blood products accelerate bone regeneration for mastoid reconstruction. Autologous SVF cells with PCL scaffolds and autologous blood products are promising composites for mastoid reconstruction which can be easily harvested after mastoidectomy. With this approach, the reconstruction of mastoid bone defects can be performed right after mastoidectomy as a one-step procedure which can offer efficiency in the clinical field. |
| first_indexed | 2024-03-09T20:25:12Z |
| format | Article |
| id | doaj.art-50a384db5b624d1c984540690d8a15f2 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T20:25:12Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-50a384db5b624d1c984540690d8a15f22023-11-23T23:37:39ZengMDPI AGPolymers2073-43602022-02-0114587710.3390/polym14050877Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media SurgeriesSung-Hee Park0Hantai Kim1Yun Yeong Lee2Yeon Ju Kim3Jeong Hun Jang4Oak-Sung Choo5Yun-Hoon Choung6Department of Otolaryngology, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Otolaryngology, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Otolaryngology, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Otolaryngology, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Otolaryngology, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Otorhinolaryngology, Eulji University School of Medicine, Uijeongbu 11759, KoreaDepartment of Otolaryngology, Ajou University School of Medicine, Suwon 16499, KoreaMastoidectomy is a surgical procedure for the treatment of chronic otitis media. This study investigated the ability of rat stromal vascular fraction cells (rSVF) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation-enhancing blood products to promote the regeneration of mastoid bone defect. Twenty male Sprague Dawley rats were randomly divided according to obliteration materials: (1) control, (2) PCL scaffold only, (3) rSVFs + PCL, (4) rSVFs + PCL + platelet-rich plasma, and (5) rSVFs + PCL + whole plasma (WP). At 7 months after transplantation, the rSVFs + PCL + WP group showed remarkable new bone formation in the mastoid. These results indicate that SVFs, PCL scaffolds, and blood products accelerate bone regeneration for mastoid reconstruction. Autologous SVF cells with PCL scaffolds and autologous blood products are promising composites for mastoid reconstruction which can be easily harvested after mastoidectomy. With this approach, the reconstruction of mastoid bone defects can be performed right after mastoidectomy as a one-step procedure which can offer efficiency in the clinical field.https://www.mdpi.com/2073-4360/14/5/877mastoidectomyosteogenesisstromal vascular fraction cellspolycaprolactone (PCL) scaffoldautologous growth factor |
| spellingShingle | Sung-Hee Park Hantai Kim Yun Yeong Lee Yeon Ju Kim Jeong Hun Jang Oak-Sung Choo Yun-Hoon Choung Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries Polymers mastoidectomy osteogenesis stromal vascular fraction cells polycaprolactone (PCL) scaffold autologous growth factor |
| title | Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries |
| title_full | Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries |
| title_fullStr | Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries |
| title_full_unstemmed | Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries |
| title_short | Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries |
| title_sort | development of intracorporeal differentiation of stem cells to induce one step mastoid bone reconstruction during otitis media surgeries |
| topic | mastoidectomy osteogenesis stromal vascular fraction cells polycaprolactone (PCL) scaffold autologous growth factor |
| url | https://www.mdpi.com/2073-4360/14/5/877 |
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