Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3
Background: Vitamin D, an important hormone required by the body, exerts its biological effects through Vitamin D receptors (VDRs) present on target cells. Vitamin D is ineffective in tissues which lack VDR. Various tissues show the presence of VDRs. However, evidence for the presence of VDRs in hum...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Indian Society of Periodontology |
| Subjects: | |
| Online Access: | http://www.jisponline.com/article.asp?issn=0972-124X;year=2019;volume=23;issue=2;spage=100;epage=105;aulast=Patil |
| _version_ | 1818504001751089152 |
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| author | Vishakha Sarang Patil Rohini Salil Mali Alpana Subodh Moghe |
| author_facet | Vishakha Sarang Patil Rohini Salil Mali Alpana Subodh Moghe |
| author_sort | Vishakha Sarang Patil |
| collection | DOAJ |
| description | Background: Vitamin D, an important hormone required by the body, exerts its biological effects through Vitamin D receptors (VDRs) present on target cells. Vitamin D is ineffective in tissues which lack VDR. Various tissues show the presence of VDRs. However, evidence for the presence of VDRs in human periodontal ligament tissue in fully erupted teeth in adults is lacking. The present study intends to evaluate the presence of VDRs in periodontal ligament (PDL) tissue and assess their response to serum Vitamin D3 levels in chronic periodontic patients. Materials and Methods: A total of 19 chronic periodontitis patients were enrolled in the study and tested for serum 25(OH)D3 levels. Deficient patients were supplemented with Vitamin D3. PDL tissue of these patients was isolated after tooth extraction before and after supplementation of Vitamin D3 and analyzed for the presence of VDR in PDL tissue by using enzyme-linked immunosorbent assay. Results: All the chronic periodontitis patients were found to be deficient in Vitamin D3. The mean serum 25(OH)D3 level before supplementation was 13.96 ng/mL which significantly increased to 35.12 ng/mL after supplementation of Vitamin D3 for 6 weeks. VDR analysis determined mean VDR conc. in PDL tissue to be -1.443 ng/mL, which increased to 2.38 ng/mL after supplementation. A concentration dependent correlation was seen between serum 25(OH)D3 levels and VDR conc. in PDL tissue after supplementation. Conclusions: The study determined Vitamin D Receptors (VDR) in PDL tissue after supplementation of Vitamin D. Thus in addition to the standard treatment modalities, Vitamin D3 supplementation would be an important factor for generation of adequate immune response. |
| first_indexed | 2024-12-10T21:31:17Z |
| format | Article |
| id | doaj.art-50a5af0789f646dfab387b522d5f7165 |
| institution | Directory Open Access Journal |
| issn | 0972-124X |
| language | English |
| last_indexed | 2024-12-10T21:31:17Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Periodontology |
| spelling | doaj.art-50a5af0789f646dfab387b522d5f71652022-12-22T01:32:48ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2019-01-0123210010510.4103/jisp.jisp_173_18Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3Vishakha Sarang PatilRohini Salil MaliAlpana Subodh MogheBackground: Vitamin D, an important hormone required by the body, exerts its biological effects through Vitamin D receptors (VDRs) present on target cells. Vitamin D is ineffective in tissues which lack VDR. Various tissues show the presence of VDRs. However, evidence for the presence of VDRs in human periodontal ligament tissue in fully erupted teeth in adults is lacking. The present study intends to evaluate the presence of VDRs in periodontal ligament (PDL) tissue and assess their response to serum Vitamin D3 levels in chronic periodontic patients. Materials and Methods: A total of 19 chronic periodontitis patients were enrolled in the study and tested for serum 25(OH)D3 levels. Deficient patients were supplemented with Vitamin D3. PDL tissue of these patients was isolated after tooth extraction before and after supplementation of Vitamin D3 and analyzed for the presence of VDR in PDL tissue by using enzyme-linked immunosorbent assay. Results: All the chronic periodontitis patients were found to be deficient in Vitamin D3. The mean serum 25(OH)D3 level before supplementation was 13.96 ng/mL which significantly increased to 35.12 ng/mL after supplementation of Vitamin D3 for 6 weeks. VDR analysis determined mean VDR conc. in PDL tissue to be -1.443 ng/mL, which increased to 2.38 ng/mL after supplementation. A concentration dependent correlation was seen between serum 25(OH)D3 levels and VDR conc. in PDL tissue after supplementation. Conclusions: The study determined Vitamin D Receptors (VDR) in PDL tissue after supplementation of Vitamin D. Thus in addition to the standard treatment modalities, Vitamin D3 supplementation would be an important factor for generation of adequate immune response.http://www.jisponline.com/article.asp?issn=0972-124X;year=2019;volume=23;issue=2;spage=100;epage=105;aulast=PatilChronic periodontitisenzyme-linked immunosorbent assayperiodontal ligament tissueVitamin D deficiencyVitamin D receptor |
| spellingShingle | Vishakha Sarang Patil Rohini Salil Mali Alpana Subodh Moghe Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3 Journal of Indian Society of Periodontology Chronic periodontitis enzyme-linked immunosorbent assay periodontal ligament tissue Vitamin D deficiency Vitamin D receptor |
| title | Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3 |
| title_full | Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3 |
| title_fullStr | Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3 |
| title_full_unstemmed | Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3 |
| title_short | Evaluation and comparison of Vitamin D receptors in periodontal ligament tissue of Vitamin D-deficient chronic periodontitis patients before and after supplementation of Vitamin D3 |
| title_sort | evaluation and comparison of vitamin d receptors in periodontal ligament tissue of vitamin d deficient chronic periodontitis patients before and after supplementation of vitamin d3 |
| topic | Chronic periodontitis enzyme-linked immunosorbent assay periodontal ligament tissue Vitamin D deficiency Vitamin D receptor |
| url | http://www.jisponline.com/article.asp?issn=0972-124X;year=2019;volume=23;issue=2;spage=100;epage=105;aulast=Patil |
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