Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer
Lung cancers are broadly divided into two categories: non-small-cell lung carcinoma (NSCLC), which accounts for 80–85% of all cancer cases, and small-cell lung carcinoma (SCLC), which covers the remaining 10–15%. Recent advances in cancer biology and genomics research have allowed an in-depth charac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5346 |
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| author | Radu Pirlog Paul Chiroi Ioana Rusu Ancuta Maria Jurj Liviuta Budisan Cecilia Pop-Bica Cornelia Braicu Doinita Crisan Jean-Christophe Sabourin Ioana Berindan-Neagoe |
| author_facet | Radu Pirlog Paul Chiroi Ioana Rusu Ancuta Maria Jurj Liviuta Budisan Cecilia Pop-Bica Cornelia Braicu Doinita Crisan Jean-Christophe Sabourin Ioana Berindan-Neagoe |
| author_sort | Radu Pirlog |
| collection | DOAJ |
| description | Lung cancers are broadly divided into two categories: non-small-cell lung carcinoma (NSCLC), which accounts for 80–85% of all cancer cases, and small-cell lung carcinoma (SCLC), which covers the remaining 10–15%. Recent advances in cancer biology and genomics research have allowed an in-depth characterization of lung cancers that have revealed new therapy targets (<i>EGFR</i>, <i>ALK</i>, <i>ROS</i>, and <i>KRAS</i> mutations) and have the potential of revealing even more biomarkers for diagnostic, prognostic, and targeted therapies. A new source of biomarkers is represented by non-coding RNAs, especially microRNAs (miRNAs). MiRNAs are short non-coding RNA sequences that have essential regulatory roles in multiple cancers. Therefore, we aim to investigate the tumor microenvironment (TME) and miRNA tumor profile in a subset of 51 early-stage lung cancer samples (T1 and T2) to better understand early tumor and TME organization and molecular dysregulation. We analyzed the immunohistochemistry expression of CD4 and CD8 as markers of the main TME immune populations, E-cadherin to evaluate early-stage epithelial-to-mesenchymal transition (EMT), and p53, the main altered tumor suppressor gene in lung cancer. Starting from these 4 markers, we identified and validated 4 miRNAs that target <i>TP53</i> and regulate EMT that can be further investigated as potential early-stage lung cancer biomarkers. |
| first_indexed | 2024-03-10T03:45:18Z |
| format | Article |
| id | doaj.art-50a9440949ef4af4a4b6bad6ffdef769 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:45:18Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-50a9440949ef4af4a4b6bad6ffdef7692023-11-23T11:21:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310534610.3390/ijms23105346Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung CancerRadu Pirlog0Paul Chiroi1Ioana Rusu2Ancuta Maria Jurj3Liviuta Budisan4Cecilia Pop-Bica5Cornelia Braicu6Doinita Crisan7Jean-Christophe Sabourin8Ioana Berindan-Neagoe9Research Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaDepartment of Pathology, Regional Institute of Gastroenterology and Hepatology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400186 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaDepartment of Morphological Sciences, “Iuliu Hațieganu” University of Medicine and Pharmacy, 6 Pasteur Street, 400349 Cluj-Napoca, RomaniaPathology Department and INSERM U1245, Rouen University Hospital, Normandy University, 76000 Rouen, FranceResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaLung cancers are broadly divided into two categories: non-small-cell lung carcinoma (NSCLC), which accounts for 80–85% of all cancer cases, and small-cell lung carcinoma (SCLC), which covers the remaining 10–15%. Recent advances in cancer biology and genomics research have allowed an in-depth characterization of lung cancers that have revealed new therapy targets (<i>EGFR</i>, <i>ALK</i>, <i>ROS</i>, and <i>KRAS</i> mutations) and have the potential of revealing even more biomarkers for diagnostic, prognostic, and targeted therapies. A new source of biomarkers is represented by non-coding RNAs, especially microRNAs (miRNAs). MiRNAs are short non-coding RNA sequences that have essential regulatory roles in multiple cancers. Therefore, we aim to investigate the tumor microenvironment (TME) and miRNA tumor profile in a subset of 51 early-stage lung cancer samples (T1 and T2) to better understand early tumor and TME organization and molecular dysregulation. We analyzed the immunohistochemistry expression of CD4 and CD8 as markers of the main TME immune populations, E-cadherin to evaluate early-stage epithelial-to-mesenchymal transition (EMT), and p53, the main altered tumor suppressor gene in lung cancer. Starting from these 4 markers, we identified and validated 4 miRNAs that target <i>TP53</i> and regulate EMT that can be further investigated as potential early-stage lung cancer biomarkers.https://www.mdpi.com/1422-0067/23/10/5346early-stage lung cancertumor microenvironmentp53E-cadherinCD4CD8 |
| spellingShingle | Radu Pirlog Paul Chiroi Ioana Rusu Ancuta Maria Jurj Liviuta Budisan Cecilia Pop-Bica Cornelia Braicu Doinita Crisan Jean-Christophe Sabourin Ioana Berindan-Neagoe Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer International Journal of Molecular Sciences early-stage lung cancer tumor microenvironment p53 E-cadherin CD4 CD8 |
| title | Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer |
| title_full | Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer |
| title_fullStr | Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer |
| title_full_unstemmed | Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer |
| title_short | Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer |
| title_sort | cellular and molecular profiling of tumor microenvironment and early stage lung cancer |
| topic | early-stage lung cancer tumor microenvironment p53 E-cadherin CD4 CD8 |
| url | https://www.mdpi.com/1422-0067/23/10/5346 |
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