Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure
<p>Metallothionein (MT) is the main protein involved in the homeostasis of metallic micronutrients and in cellular defence against heavy metals and reactive oxygen species. Found in almost all vertebrate tissues, MT presence and localization in the testis has been controversial. In the present...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2017-05-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | http://ejh.it/index.php/ejh/article/view/2777 |
| _version_ | 1818530951849836544 |
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| author | Mariailaria Verderame Ermelinda Limatola Rosaria Scudiero |
| author_facet | Mariailaria Verderame Ermelinda Limatola Rosaria Scudiero |
| author_sort | Mariailaria Verderame |
| collection | DOAJ |
| description | <p>Metallothionein (MT) is the main protein involved in the homeostasis of metallic micronutrients and in cellular defence against heavy metals and reactive oxygen species. Found in almost all vertebrate tissues, MT presence and localization in the testis has been controversial. In the present study, by using <em>in situ</em> hybridization and immunohistochemical analysis we assessed the localization of both MT transcript and protein in <em>Podarcis sicula</em> testes during two different phases of the reproductive cycle: the autumnal resumption and the spring-early summer mating period. In addition, with the same methodological approach, we verified the effect of estradiol-17ß and nonylphenol, a potent xenoestrogen, on MT expression and synthesis. These results, the first collected in a non-mammalian oviparous vertebrate, demonstrated that the expression profile of MT mRNA and protein changes during the reproductive cycle. In the fall resumption, MT transcripts are absent in spermatogonia and present in all the other cells of tubules, including spermatozoa; <em>vice versa</em>, the MT protein is evident only in spermatozoa and somatic cells. In the mating period, both MT transcripts and proteins are present in spermatogonia, spermatocytes and spermatids, whereas in the spermatozoa only the proteins are detected, thus suggesting that the MTs translated in the earlier germinal stages are stored up to spermatozoa. Results also demonstrated that in lizard testis the expression of MT gene undergoes a cell-specific regulation after estrogenic exposure; the possible role and the mechanism by which this regulation occurs have been discussed.</p> |
| first_indexed | 2024-12-11T17:26:11Z |
| format | Article |
| id | doaj.art-50a9eea157434b8785aaa14618c46531 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T17:26:11Z |
| publishDate | 2017-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-50a9eea157434b8785aaa14618c465312022-12-22T00:56:58ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062017-05-0161210.4081/ejh.2017.27771549Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposureMariailaria Verderame0Ermelinda Limatola1Rosaria Scudiero2University of Naples Federico II, Department of BiologyUniversity of Naples Federico II, Department of BiologyUniversity of Naples Federico II, Department of Biology<p>Metallothionein (MT) is the main protein involved in the homeostasis of metallic micronutrients and in cellular defence against heavy metals and reactive oxygen species. Found in almost all vertebrate tissues, MT presence and localization in the testis has been controversial. In the present study, by using <em>in situ</em> hybridization and immunohistochemical analysis we assessed the localization of both MT transcript and protein in <em>Podarcis sicula</em> testes during two different phases of the reproductive cycle: the autumnal resumption and the spring-early summer mating period. In addition, with the same methodological approach, we verified the effect of estradiol-17ß and nonylphenol, a potent xenoestrogen, on MT expression and synthesis. These results, the first collected in a non-mammalian oviparous vertebrate, demonstrated that the expression profile of MT mRNA and protein changes during the reproductive cycle. In the fall resumption, MT transcripts are absent in spermatogonia and present in all the other cells of tubules, including spermatozoa; <em>vice versa</em>, the MT protein is evident only in spermatozoa and somatic cells. In the mating period, both MT transcripts and proteins are present in spermatogonia, spermatocytes and spermatids, whereas in the spermatozoa only the proteins are detected, thus suggesting that the MTs translated in the earlier germinal stages are stored up to spermatozoa. Results also demonstrated that in lizard testis the expression of MT gene undergoes a cell-specific regulation after estrogenic exposure; the possible role and the mechanism by which this regulation occurs have been discussed.</p>http://ejh.it/index.php/ejh/article/view/2777metallothionein, spermatogenesis, reptiles, estrogen, nonylphenol, in situ hybridization, immunohistochemistry |
| spellingShingle | Mariailaria Verderame Ermelinda Limatola Rosaria Scudiero Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure European Journal of Histochemistry metallothionein, spermatogenesis, reptiles, estrogen, nonylphenol, in situ hybridization, immunohistochemistry |
| title | Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure |
| title_full | Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure |
| title_fullStr | Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure |
| title_full_unstemmed | Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure |
| title_short | Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure |
| title_sort | metallothionein expression and synthesis in the testis of the lizard podarcis sicula under natural conditions and following estrogenic exposure |
| topic | metallothionein, spermatogenesis, reptiles, estrogen, nonylphenol, in situ hybridization, immunohistochemistry |
| url | http://ejh.it/index.php/ejh/article/view/2777 |
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