17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)
The aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Ictiologia
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| Series: | Neotropical Ichthyology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300613&lng=en&tlng=en |
| _version_ | 1811239825526751232 |
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| author | Denise Vizziano Cantonnet Magdalena Mateo Andrés Alberro Florencia Barrios Alexis Fostier |
| author_facet | Denise Vizziano Cantonnet Magdalena Mateo Andrés Alberro Florencia Barrios Alexis Fostier |
| author_sort | Denise Vizziano Cantonnet |
| collection | DOAJ |
| description | The aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3H-17-hydroxy-4-pregnene-3,20-dione precursor through thin layer chromatography, high pressure liquid chromatography, enzymatic oxydation, acetylation and immunochemistry analyses. 17,20β-Dihydroxy-4-pregnen-3-one (17,20β-P) and 11β,17,21-Trihydroxy-4-pregnene-3,20-dione (cortisol) were the main metabolites produced. Contrary to what we expected, 17,20β,21-Trihydroxy-4-pregnen-3-one was not detected. Circulating levels of 17,20β-P were undetectable in immature testes and in those at the first spermatogenesis stages, while a clear increase was observed during the whole spermatogenesis and spermiation phases (from undetectable to 1047 pg mL-1). In vitro studies together with plasma detection suggest that 17,20β-P is a good steroid candidate involved in M. furnieri testes maturation. The role of cortisol during late phases of testes development needs further studies. |
| first_indexed | 2024-04-12T13:07:56Z |
| format | Article |
| id | doaj.art-50aa2bdf8a4a44e1b4fc6fa5c039078a |
| institution | Directory Open Access Journal |
| issn | 1982-0224 |
| language | English |
| last_indexed | 2024-04-12T13:07:56Z |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj.art-50aa2bdf8a4a44e1b4fc6fa5c039078a2022-12-22T03:31:56ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-022413361362410.1590/1982-0224-20150013S1679-6225201500030061317,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)Denise Vizziano CantonnetMagdalena MateoAndrés AlberroFlorencia BarriosAlexis FostierThe aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3H-17-hydroxy-4-pregnene-3,20-dione precursor through thin layer chromatography, high pressure liquid chromatography, enzymatic oxydation, acetylation and immunochemistry analyses. 17,20β-Dihydroxy-4-pregnen-3-one (17,20β-P) and 11β,17,21-Trihydroxy-4-pregnene-3,20-dione (cortisol) were the main metabolites produced. Contrary to what we expected, 17,20β,21-Trihydroxy-4-pregnen-3-one was not detected. Circulating levels of 17,20β-P were undetectable in immature testes and in those at the first spermatogenesis stages, while a clear increase was observed during the whole spermatogenesis and spermiation phases (from undetectable to 1047 pg mL-1). In vitro studies together with plasma detection suggest that 17,20β-P is a good steroid candidate involved in M. furnieri testes maturation. The role of cortisol during late phases of testes development needs further studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300613&lng=en&tlng=enMale gonadMaturing steroidsReproductionSteroid synthesisWhite croaker |
| spellingShingle | Denise Vizziano Cantonnet Magdalena Mateo Andrés Alberro Florencia Barrios Alexis Fostier 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) Neotropical Ichthyology Male gonad Maturing steroids Reproduction Steroid synthesis White croaker |
| title | 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) |
| title_full | 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) |
| title_fullStr | 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) |
| title_full_unstemmed | 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) |
| title_short | 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) |
| title_sort | 17 20β p and cortisol are the main in vitro metabolites of 17 hydroxy progesterone produced by spermiating testes of micropogonias furnieri desmarest 1823 perciformes sciaenidae |
| topic | Male gonad Maturing steroids Reproduction Steroid synthesis White croaker |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300613&lng=en&tlng=en |
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