The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signaling
Abstract Introduction Despite half a century of research on vitamin D (Vit. D), its link to substance abuse and dependence has only been discussed in recent decades. Evidence also shows the involvement of Vit. D in the evolution of dopaminergic neurons in the nucleus accumbens, an increase in the ex...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | Brain and Behavior |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/brb3.2877 |
| _version_ | 1811164239547596800 |
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| author | Mahbubeh Saeedfar Abolfazl Ardjmand Behrang Alani Amir Ghaderi Hamid Reza Banafshe Mohammad Esmaeil Shahaboddin Gholamreza Ghavipanjeh |
| author_facet | Mahbubeh Saeedfar Abolfazl Ardjmand Behrang Alani Amir Ghaderi Hamid Reza Banafshe Mohammad Esmaeil Shahaboddin Gholamreza Ghavipanjeh |
| author_sort | Mahbubeh Saeedfar |
| collection | DOAJ |
| description | Abstract Introduction Despite half a century of research on vitamin D (Vit. D), its link to substance abuse and dependence has only been discussed in recent decades. Evidence also shows the involvement of Vit. D in the evolution of dopaminergic neurons in the nucleus accumbens, an increase in the expression of tyrosine hydroxylase, and the regulation of dopaminergic processes. The novel idea for this work is taken from a hypothesis given about the effectiveness of Vit. D on dopamine signaling pathway. It is therefore presumed that Vit. D can be considered an effective therapeutic approach for narcotic addiction and substance abuse. Methods The animals were assigned into six groups (control, vehicle, Morphine [Mor.], and Vit. D [250, 500, and 1000 IU/kg, i.p.]). Following each conditioning session in a conditioned place preference (CPP) model, the animals received Vit. D. Afterward, the locomotor activity of the animals was assessed using open‐field apparatus. Malondialdehyde (MDA), nitric oxide (NO), catalase (CAT), superoxide dismutase (SOD), thiol, and total antioxidant capacity (TAC) were measured in the brain. The relative DRD2 and GDNF expressions (%) were also measured in the hippocampus. Results Vit. D administration after Mor. caused a significant increase in the place preference index in the acquisition phase (p < .05). Vit. D altered the oxidation/antioxidation profiles (CAT, SOD, MDA, NO, TAC, and Thiol). Vit. D was more effective than Mor. in the expression of GDNF (p < .0001); however, in the expression of DRD2, this was only the case for 1000 IU Vit. D (p < .0001). Conclusions Considering the increased place preference index induced by Mor., it can be concluded that Vit. D interacts via the oxidative pathway and DRD2–GDNF signaling to potentiate the Mor. effect. |
| first_indexed | 2024-04-10T15:19:18Z |
| format | Article |
| id | doaj.art-50aafb4357dd468a899879182b2c0834 |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-04-10T15:19:18Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-50aafb4357dd468a899879182b2c08342023-02-14T16:52:41ZengWileyBrain and Behavior2162-32792023-02-01132n/an/a10.1002/brb3.2877The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signalingMahbubeh Saeedfar0Abolfazl Ardjmand1Behrang Alani2Amir Ghaderi3Hamid Reza Banafshe4Mohammad Esmaeil Shahaboddin5Gholamreza Ghavipanjeh6Institute for Basic Sciences, Physiology Research Center Kashan University of Medical Sciences Kashan IranInstitute for Basic Sciences, Physiology Research Center Kashan University of Medical Sciences Kashan IranDepartment of Applied Cell Sciences, Faculty of Medicine Kashan University of Medical Sciences Kashan IranDepartment of Addiction Studies, School of Medical Kashan University of Medical Sciences Kashan IranInstitute for Basic Sciences, Physiology Research Center Kashan University of Medical Sciences Kashan IranResearch Center for Biochemistry and Nutrition in Metabolic Diseases Kashan University of Medical Sciences Kashan IranInstitute for Basic Sciences, Physiology Research Center Kashan University of Medical Sciences Kashan IranAbstract Introduction Despite half a century of research on vitamin D (Vit. D), its link to substance abuse and dependence has only been discussed in recent decades. Evidence also shows the involvement of Vit. D in the evolution of dopaminergic neurons in the nucleus accumbens, an increase in the expression of tyrosine hydroxylase, and the regulation of dopaminergic processes. The novel idea for this work is taken from a hypothesis given about the effectiveness of Vit. D on dopamine signaling pathway. It is therefore presumed that Vit. D can be considered an effective therapeutic approach for narcotic addiction and substance abuse. Methods The animals were assigned into six groups (control, vehicle, Morphine [Mor.], and Vit. D [250, 500, and 1000 IU/kg, i.p.]). Following each conditioning session in a conditioned place preference (CPP) model, the animals received Vit. D. Afterward, the locomotor activity of the animals was assessed using open‐field apparatus. Malondialdehyde (MDA), nitric oxide (NO), catalase (CAT), superoxide dismutase (SOD), thiol, and total antioxidant capacity (TAC) were measured in the brain. The relative DRD2 and GDNF expressions (%) were also measured in the hippocampus. Results Vit. D administration after Mor. caused a significant increase in the place preference index in the acquisition phase (p < .05). Vit. D altered the oxidation/antioxidation profiles (CAT, SOD, MDA, NO, TAC, and Thiol). Vit. D was more effective than Mor. in the expression of GDNF (p < .0001); however, in the expression of DRD2, this was only the case for 1000 IU Vit. D (p < .0001). Conclusions Considering the increased place preference index induced by Mor., it can be concluded that Vit. D interacts via the oxidative pathway and DRD2–GDNF signaling to potentiate the Mor. effect.https://doi.org/10.1002/brb3.2877Condition place preferenceDopamineMorphineRatVitamin D |
| spellingShingle | Mahbubeh Saeedfar Abolfazl Ardjmand Behrang Alani Amir Ghaderi Hamid Reza Banafshe Mohammad Esmaeil Shahaboddin Gholamreza Ghavipanjeh The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signaling Brain and Behavior Condition place preference Dopamine Morphine Rat Vitamin D |
| title | The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signaling |
| title_full | The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signaling |
| title_fullStr | The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signaling |
| title_full_unstemmed | The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signaling |
| title_short | The effect of vitamin D on morphine preference in rats: Possible biochemical and DRD2–GDNF signaling |
| title_sort | effect of vitamin d on morphine preference in rats possible biochemical and drd2 gdnf signaling |
| topic | Condition place preference Dopamine Morphine Rat Vitamin D |
| url | https://doi.org/10.1002/brb3.2877 |
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