Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats
Objective: Postmenopausal osteoporosis is characterized by increased fracture risk. However, each approved treatment has specific side effects. Therefore, foods with plant origins have increasingly attracted attention as an alternative treatment. Studies have shown that Elaeagnus angustifolia (EA) h...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2017-04-01
|
| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | http://ajp.mums.ac.ir/article_8195_a8570fc32c8c44ac6c659bd7839ddd77.pdf |
| _version_ | 1818213210563543040 |
|---|---|
| author | Mohammad Dabbaghmanesh Ali Noorafshan Pedram Talezadeh Nader Tanideh Farhad Koohpeyma Aida Iraji Marzieh Bakhshayeshkaram Nima Montazeri-Najafabady |
| author_facet | Mohammad Dabbaghmanesh Ali Noorafshan Pedram Talezadeh Nader Tanideh Farhad Koohpeyma Aida Iraji Marzieh Bakhshayeshkaram Nima Montazeri-Najafabady |
| author_sort | Mohammad Dabbaghmanesh |
| collection | DOAJ |
| description | Objective: Postmenopausal osteoporosis is characterized by increased fracture risk. However, each approved treatment has specific side effects. Therefore, foods with plant origins have increasingly attracted attention as an alternative treatment. Studies have shown that Elaeagnus angustifolia (EA) has antioxidant properties. The present study aimed to investigate the effects of EA hydroalcoholic extract on ovariectomy-induced osteoporosis in rats using stereological methods. Material and Methods: 55 female Sprague-Dawley rats were randomly assigned to control, sham operated (normal saline), ovariectomized (OVX), OVX + EA fruit extract (600 mg/kg BW/day), and OVX + estradiol benzoate (3 mg/kg BW) for 16 weeks. Blood samples were collected to measure calcium, phosphorus, and alkaline phosphatase (ALP) plasma levels. Then, specimens from tibia and fifth lumbar vertebra (L5) bones were prepared and stereological analysis was done. Results: Ovariectomy significantly decreased the calcium level and increased the ALP level in the OVX group. In spite of improvement in calcium hemostasis in groups treated with estrogen and EA fruit extract (p |
| first_indexed | 2024-12-12T06:00:40Z |
| format | Article |
| id | doaj.art-50ab1741827c4053899b46bba58db6d8 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-12T06:00:40Z |
| publishDate | 2017-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-50ab1741827c4053899b46bba58db6d82022-12-22T00:35:25ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492017-04-017326127410.22038/ajp.2017.15516.16178195Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized ratsMohammad Dabbaghmanesh0Ali Noorafshan1Pedram Talezadeh2Nader Tanideh3Farhad Koohpeyma4Aida Iraji5Marzieh Bakhshayeshkaram6Nima Montazeri-Najafabady7Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Anatomy, Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of pharmacology, Stem Cell and Transgenic Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IranResearcher in Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, IranResearcher in Health Policy Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IranObjective: Postmenopausal osteoporosis is characterized by increased fracture risk. However, each approved treatment has specific side effects. Therefore, foods with plant origins have increasingly attracted attention as an alternative treatment. Studies have shown that Elaeagnus angustifolia (EA) has antioxidant properties. The present study aimed to investigate the effects of EA hydroalcoholic extract on ovariectomy-induced osteoporosis in rats using stereological methods. Material and Methods: 55 female Sprague-Dawley rats were randomly assigned to control, sham operated (normal saline), ovariectomized (OVX), OVX + EA fruit extract (600 mg/kg BW/day), and OVX + estradiol benzoate (3 mg/kg BW) for 16 weeks. Blood samples were collected to measure calcium, phosphorus, and alkaline phosphatase (ALP) plasma levels. Then, specimens from tibia and fifth lumbar vertebra (L5) bones were prepared and stereological analysis was done. Results: Ovariectomy significantly decreased the calcium level and increased the ALP level in the OVX group. In spite of improvement in calcium hemostasis in groups treated with estrogen and EA fruit extract (phttp://ajp.mums.ac.ir/article_8195_a8570fc32c8c44ac6c659bd7839ddd77.pdfElaeagnus angustifoliaStereologyosteoporosisTibia boneALPRat |
| spellingShingle | Mohammad Dabbaghmanesh Ali Noorafshan Pedram Talezadeh Nader Tanideh Farhad Koohpeyma Aida Iraji Marzieh Bakhshayeshkaram Nima Montazeri-Najafabady Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats Avicenna Journal of Phytomedicine Elaeagnus angustifolia Stereology osteoporosis Tibia bone ALP Rat |
| title | Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats |
| title_full | Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats |
| title_fullStr | Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats |
| title_full_unstemmed | Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats |
| title_short | Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats |
| title_sort | stereological investigation of the effect of elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats |
| topic | Elaeagnus angustifolia Stereology osteoporosis Tibia bone ALP Rat |
| url | http://ajp.mums.ac.ir/article_8195_a8570fc32c8c44ac6c659bd7839ddd77.pdf |
| work_keys_str_mv | AT mohammaddabbaghmanesh stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats AT alinoorafshan stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats AT pedramtalezadeh stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats AT nadertanideh stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats AT farhadkoohpeyma stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats AT aidairaji stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats AT marziehbakhshayeshkaram stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats AT nimamontazerinajafabady stereologicalinvestigationoftheeffectofelaeagnusangustifoliafruithydroalcoholicextractonosteoporosisinovariectomizedrats |