Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm Birth
Preterm birth (PTB), as the leading cause of neonatal death, is a severe threat to maternal–fetal health. The diagnosis and treatment of PTB are difficult as its underlying mechanism still unknown. Circular RNA (circRNA) is an emerging molecule that plays an essential role in the pathological proces...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2020.566984/full |
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| author | Yuxin Ran Yuxin Ran Yuxin Ran Nanlin Yin Nanlin Yin Nanlin Yin Nanlin Yin Dongni Huang Dongni Huang Dongni Huang Yangyu Zhao Jing Yang Hanwen Zhang Hanwen Zhang Hanwen Zhang Hongbo Qi Hongbo Qi Hongbo Qi Hongbo Qi |
| author_facet | Yuxin Ran Yuxin Ran Yuxin Ran Nanlin Yin Nanlin Yin Nanlin Yin Nanlin Yin Dongni Huang Dongni Huang Dongni Huang Yangyu Zhao Jing Yang Hanwen Zhang Hanwen Zhang Hanwen Zhang Hongbo Qi Hongbo Qi Hongbo Qi Hongbo Qi |
| author_sort | Yuxin Ran |
| collection | DOAJ |
| description | Preterm birth (PTB), as the leading cause of neonatal death, is a severe threat to maternal–fetal health. The diagnosis and treatment of PTB are difficult as its underlying mechanism still unknown. Circular RNA (circRNA) is an emerging molecule that plays an essential role in the pathological processes of various diseases. However, it is still unclear whether circRNAs are abnormal or involves in the PTB pathology. In this study, we analyzed RNA-seq data of peripheral blood from preterm and term pregnant women and verified with microarray data. There were 211 circRNA expression disorders in PTB, of which 68 increased and 143 decreased. Bioinformatics analysis revealed that the top 20 circRNAs competitively bind 68 miRNAs, thereby regulating 622 mRNAs mainly related to immunity, inflammation, and nerve activity, which may ultimately contribute to the occurrence of PTB. Moreover, 6 regulatory pairs, including hsa-MORC3_0001–hsa-miR-1248–CHRM2 were the core parts of this mechanism network, which might be therapeutic targets for PTB. Besides, ROC analysis indicated that hsa-ANKFY1_0025, hsa-FAM13B_0019, and hsa-NUSAP1_0010 (AUC = 0.7138, 0.9589, 1.000) have an excellent discrimination ability for PTB. Taken together, we explored for the first time the circRNA expression profile of PTB, and preliminarily analyzed its regulatory mechanism and predictive value for PTB, thus bringing new light to the diagnosis and treatment of PTB. |
| first_indexed | 2024-12-14T04:47:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-14T04:47:43Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-50ae7366db5a45cca833b001bf06cbfb2022-12-21T23:16:37ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-12-01810.3389/fbioe.2020.566984566984Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm BirthYuxin Ran0Yuxin Ran1Yuxin Ran2Nanlin Yin3Nanlin Yin4Nanlin Yin5Nanlin Yin6Dongni Huang7Dongni Huang8Dongni Huang9Yangyu Zhao10Jing Yang11Hanwen Zhang12Hanwen Zhang13Hanwen Zhang14Hongbo Qi15Hongbo Qi16Hongbo Qi17Hongbo Qi18Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, ChinaJoint International Research Laboratory of Reproduction and Development of Chinese Ministry of Education, Chongqing Medical University, Chongqing, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, ChinaJoint International Research Laboratory of Reproduction and Development of Chinese Ministry of Education, Chongqing Medical University, Chongqing, ChinaCenter for Reproductive Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, ChinaJoint International Research Laboratory of Reproduction and Development of Chinese Ministry of Education, Chongqing Medical University, Chongqing, ChinaDepartment of Obstetrics & Gynecology, Peking University Third Hospital, Beijing, ChinaDepartment of Obstetrics & Gynecology, Peking University Third Hospital, Beijing, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, ChinaJoint International Research Laboratory of Reproduction and Development of Chinese Ministry of Education, Chongqing Medical University, Chongqing, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, ChinaJoint International Research Laboratory of Reproduction and Development of Chinese Ministry of Education, Chongqing Medical University, Chongqing, ChinaCenter for Reproductive Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaPreterm birth (PTB), as the leading cause of neonatal death, is a severe threat to maternal–fetal health. The diagnosis and treatment of PTB are difficult as its underlying mechanism still unknown. Circular RNA (circRNA) is an emerging molecule that plays an essential role in the pathological processes of various diseases. However, it is still unclear whether circRNAs are abnormal or involves in the PTB pathology. In this study, we analyzed RNA-seq data of peripheral blood from preterm and term pregnant women and verified with microarray data. There were 211 circRNA expression disorders in PTB, of which 68 increased and 143 decreased. Bioinformatics analysis revealed that the top 20 circRNAs competitively bind 68 miRNAs, thereby regulating 622 mRNAs mainly related to immunity, inflammation, and nerve activity, which may ultimately contribute to the occurrence of PTB. Moreover, 6 regulatory pairs, including hsa-MORC3_0001–hsa-miR-1248–CHRM2 were the core parts of this mechanism network, which might be therapeutic targets for PTB. Besides, ROC analysis indicated that hsa-ANKFY1_0025, hsa-FAM13B_0019, and hsa-NUSAP1_0010 (AUC = 0.7138, 0.9589, 1.000) have an excellent discrimination ability for PTB. Taken together, we explored for the first time the circRNA expression profile of PTB, and preliminarily analyzed its regulatory mechanism and predictive value for PTB, thus bringing new light to the diagnosis and treatment of PTB.https://www.frontiersin.org/articles/10.3389/fbioe.2020.566984/fullpreterm birthcircRNAceRNA networkmechanismprediction |
| spellingShingle | Yuxin Ran Yuxin Ran Yuxin Ran Nanlin Yin Nanlin Yin Nanlin Yin Nanlin Yin Dongni Huang Dongni Huang Dongni Huang Yangyu Zhao Jing Yang Hanwen Zhang Hanwen Zhang Hanwen Zhang Hongbo Qi Hongbo Qi Hongbo Qi Hongbo Qi Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm Birth Frontiers in Bioengineering and Biotechnology preterm birth circRNA ceRNA network mechanism prediction |
| title | Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm Birth |
| title_full | Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm Birth |
| title_fullStr | Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm Birth |
| title_full_unstemmed | Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm Birth |
| title_short | Identification and Characterization of Circular RNA as a Novel Regulator and Biomarker in Preterm Birth |
| title_sort | identification and characterization of circular rna as a novel regulator and biomarker in preterm birth |
| topic | preterm birth circRNA ceRNA network mechanism prediction |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2020.566984/full |
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