| Summary: | <p>Abstract</p> <p>Background</p> <p><it>Staphylococcus epidermidis </it>(SE) has emerged as one of the most important causes of nosocomial infections. The SaeRS two-component signal transduction system (TCS) influences virulence and biofilm formation in <it>Staphylococcus aureus</it>. The deletion of <it>saeR </it>in <it>S. epidermidis </it>results in impaired anaerobic growth and decreased nitrate utilization. However, the regulatory function of SaeRS on biofilm formation and autolysis in <it>S. epidermidis </it>remains unclear.</p> <p>Results</p> <p>The <it>saeRS </it>genes of SE1457 were deleted by homologous recombination. The <it>saeRS </it>deletion mutant, SE1457<it>ΔsaeRS</it>, exhibited increased biofilm formation that was disturbed more severely (a 4-fold reduction) by DNase I treatment compared to SE1457 and the complementation strain SE1457<it>saec</it>. Compared to SE1457 and SE1457<it>saec</it>, SE1457<it>ΔsaeRS </it>showed increased Triton X-100-induced autolysis (approximately 3-fold) and decreased cell viability in planktonic/biofilm states; further, SE1457<it>ΔsaeRS </it>also released more extracellular DNA (eDNA) in the biofilms. Correlated with the increased autolysis phenotype, the transcription of autolysis-related genes, such as <it>atlE </it>and <it>aae</it>, was increased in SE1457<it>ΔsaeRS</it>. Whereas the expression of accumulation-associated protein was up-regulated by 1.8-fold in 1457<it>ΔsaeRS</it>, the expression of an N-acetylglucosaminyl transferase enzyme (encoded by <it>icaA</it>) critical for polysaccharide intercellular adhesin (PIA) synthesis was not affected by the deletion of <it>saeRS.</it></p> <p>Conclusions</p> <p>Deletion of <it>saeRS </it>in <it>S. epidermidis </it>resulted in an increase in biofilm-forming ability, which was associated with increased eDNA release and up-regulated Aap expression. The increased eDNA release from SE1457<it>ΔsaeRS </it>was associated with increased bacterial autolysis and decreased bacterial cell viability in the planktonic/biofilm states.</p>
|
|---|