The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infant
While infant formula is usually bovine milk-based, interest in other ruminant milk-based formulas is growing. However, whether different ruminant milk treatments with varying nutrient compositions influence the infant’s brain development remains unknown. The aim was to determine the effects of consu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.937845/full |
| _version_ | 1811215563045732352 |
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| author | Ankita Jena Ankita Jena Ankita Jena Carlos A. Montoya Carlos A. Montoya Wayne Young Wayne Young Wayne Young Jane A. Mullaney Jane A. Mullaney Jane A. Mullaney Debashree Roy Ryan N. Dilger Caroline Giezenaar Caroline Giezenaar Warren C. McNabb Warren C. McNabb Nicole C. Roy Nicole C. Roy Nicole C. Roy |
| author_facet | Ankita Jena Ankita Jena Ankita Jena Carlos A. Montoya Carlos A. Montoya Wayne Young Wayne Young Wayne Young Jane A. Mullaney Jane A. Mullaney Jane A. Mullaney Debashree Roy Ryan N. Dilger Caroline Giezenaar Caroline Giezenaar Warren C. McNabb Warren C. McNabb Nicole C. Roy Nicole C. Roy Nicole C. Roy |
| author_sort | Ankita Jena |
| collection | DOAJ |
| description | While infant formula is usually bovine milk-based, interest in other ruminant milk-based formulas is growing. However, whether different ruminant milk treatments with varying nutrient compositions influence the infant’s brain development remains unknown. The aim was to determine the effects of consuming bovine, caprine, or ovine milk on brain gene expression in the early postnatal period using a pig model of the human infant. Starting at postnatal day 7 or 8, pigs were exclusively fed bovine, ovine, or caprine milk for 15 days. The mRNA abundance of 77 genes in the prefrontal cortex, hippocampus, and striatum regions was measured at postnatal day 21 or 22 using NanoString. The expression level of two hippocampal and nine striatal genes was most affected by milk treatments, particularly ovine milk. These modulatory genes are involved in glutamate, gamma-aminobutyric acid, serotonin, adrenaline and neurotrophin signaling and the synaptic vesicle cycle. The expression level of genes involved in gamma-aminobutyric acid signaling was associated with pigs’ lactose intake. In contrast, milk treatments did not affect the mRNA abundance of the genes in the prefrontal cortex. This study provides the first evidence of the association of different ruminant milk treatments with brain gene expression related to cognitive function in the first 3 months of postnatal life. |
| first_indexed | 2024-04-12T06:24:37Z |
| format | Article |
| id | doaj.art-50b430778a044ee9bb6a0ce51c41c0f2 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-12T06:24:37Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-50b430778a044ee9bb6a0ce51c41c0f22022-12-22T03:44:12ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-08-011610.3389/fnins.2022.937845937845The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infantAnkita Jena0Ankita Jena1Ankita Jena2Carlos A. Montoya3Carlos A. Montoya4Wayne Young5Wayne Young6Wayne Young7Jane A. Mullaney8Jane A. Mullaney9Jane A. Mullaney10Debashree Roy11Ryan N. Dilger12Caroline Giezenaar13Caroline Giezenaar14Warren C. McNabb15Warren C. McNabb16Nicole C. Roy17Nicole C. Roy18Nicole C. Roy19Riddet Institute, Massey University, Palmerston North, New ZealandSchool of Food and Advanced Technology, College of Sciences, Massey University, Palmerston North, New ZealandAgResearch, Palmerston North, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandAgResearch, Palmerston North, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandAgResearch, Palmerston North, New ZealandHigh-Value Nutrition National Science Challenge, Auckland, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandAgResearch, Palmerston North, New ZealandHigh-Value Nutrition National Science Challenge, Auckland, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandDepartment of Animal Sciences, University of Illinois, Urbana, IL, United StatesRiddet Institute, Massey University, Palmerston North, New ZealandFood Experience and Sensory Testing (FEAST) Laboratory, School of Food and Advanced Technology, Massey University, Palmerston North, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandHigh-Value Nutrition National Science Challenge, Auckland, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandHigh-Value Nutrition National Science Challenge, Auckland, New ZealandDepartment of Human Nutrition, University of Otago, Dunedin, New ZealandWhile infant formula is usually bovine milk-based, interest in other ruminant milk-based formulas is growing. However, whether different ruminant milk treatments with varying nutrient compositions influence the infant’s brain development remains unknown. The aim was to determine the effects of consuming bovine, caprine, or ovine milk on brain gene expression in the early postnatal period using a pig model of the human infant. Starting at postnatal day 7 or 8, pigs were exclusively fed bovine, ovine, or caprine milk for 15 days. The mRNA abundance of 77 genes in the prefrontal cortex, hippocampus, and striatum regions was measured at postnatal day 21 or 22 using NanoString. The expression level of two hippocampal and nine striatal genes was most affected by milk treatments, particularly ovine milk. These modulatory genes are involved in glutamate, gamma-aminobutyric acid, serotonin, adrenaline and neurotrophin signaling and the synaptic vesicle cycle. The expression level of genes involved in gamma-aminobutyric acid signaling was associated with pigs’ lactose intake. In contrast, milk treatments did not affect the mRNA abundance of the genes in the prefrontal cortex. This study provides the first evidence of the association of different ruminant milk treatments with brain gene expression related to cognitive function in the first 3 months of postnatal life.https://www.frontiersin.org/articles/10.3389/fnins.2022.937845/fullnutritionearly lifecognitionpiggut-brain axisbrain gene expression |
| spellingShingle | Ankita Jena Ankita Jena Ankita Jena Carlos A. Montoya Carlos A. Montoya Wayne Young Wayne Young Wayne Young Jane A. Mullaney Jane A. Mullaney Jane A. Mullaney Debashree Roy Ryan N. Dilger Caroline Giezenaar Caroline Giezenaar Warren C. McNabb Warren C. McNabb Nicole C. Roy Nicole C. Roy Nicole C. Roy The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infant Frontiers in Neuroscience nutrition early life cognition pig gut-brain axis brain gene expression |
| title | The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infant |
| title_full | The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infant |
| title_fullStr | The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infant |
| title_full_unstemmed | The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infant |
| title_short | The effects of ruminant milk treatments on hippocampal, striatal, and prefrontal cortex gene expression in pigs as a model for the human infant |
| title_sort | effects of ruminant milk treatments on hippocampal striatal and prefrontal cortex gene expression in pigs as a model for the human infant |
| topic | nutrition early life cognition pig gut-brain axis brain gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.937845/full |
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