Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i>
<i>Staphylococcus</i> is a major bacterial species that contaminates retail meat products. The objective of this study was to clarify the prevalence, antimicrobial resistance and genetic determinants of <i>Staphylococcus</i>/<i>Mammaliicoccus</i> species in retail...
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MDPI AG
2022-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/4/469 |
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| author | Masako Osada Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Nobumichi Kobayashi |
| author_facet | Masako Osada Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Nobumichi Kobayashi |
| author_sort | Masako Osada |
| collection | DOAJ |
| description | <i>Staphylococcus</i> is a major bacterial species that contaminates retail meat products. The objective of this study was to clarify the prevalence, antimicrobial resistance and genetic determinants of <i>Staphylococcus</i>/<i>Mammaliicoccus</i> species in retail ground meat in Japan. From a total of 146 retail ground meat samples (chicken, pork, mixed beef/pork) purchased during a 5-month period, 10 <i>S. aureus</i> and 112 isolates of coagulase-negative staphylococcus (CoNS)/<i>Mammaliicoccus</i> comprising 20 species were recovered. <i>S. aureus</i> isolates were classified into five genetic types, i.e., <i>coa</i>-IIa/ST5, <i>coa</i>-VIc/ST352 (CC97), <i>coa</i>-VIIb/ST398, <i>coa</i>-Xa/ST15, and <i>coa</i>-XIc/ST9, which were all related to those of livestock-associated clones. All the staphylococcal isolates were <i>mecA</i>-negative and mostly susceptible to all the antimicrobials tested, except for ampicillin among <i>S. aureus</i> (resistance proportion; 50%). Among CoNS, the fosfomycin resistance gene <i>fosB</i> was prevalent (30/112; 26.8%), primarily in <i>S. capitis</i>, <i>S. warneri</i>, and <i>S. saprophyticus</i>. Phylogenetic analysis of <i>fosB</i> revealed the presence of seven clusters, showing broad diversity with 65–81% identity among different clusters. In the CoNS isolates from ground meat samples, <i>fosB</i> was assigned into three clusters, and <i>S. saprophyticus</i> harbored the most divergent <i>fosB</i> with three genetic groups. These findings suggested the circulation of multiple <i>fosB</i>-carrying plasmids among some CoNS species. |
| first_indexed | 2024-03-09T13:10:30Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T13:10:30Z |
| publishDate | 2022-04-01 |
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| series | Pathogens |
| spelling | doaj.art-50b5df392f514632a522beff97f7252d2023-11-30T21:42:33ZengMDPI AGPathogens2076-08172022-04-0111446910.3390/pathogens11040469Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i>Masako Osada0Meiji Soe Aung1Noriko Urushibara2Mitsuyo Kawaguchiya3Nobuhide Ohashi4Mina Hirose5Nobumichi Kobayashi6Master’s Program in Midwifery, Tenshi College Graduate School, Sapporo 065-0013, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanDivision of Pediatric Dentistry, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 061-0293, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan<i>Staphylococcus</i> is a major bacterial species that contaminates retail meat products. The objective of this study was to clarify the prevalence, antimicrobial resistance and genetic determinants of <i>Staphylococcus</i>/<i>Mammaliicoccus</i> species in retail ground meat in Japan. From a total of 146 retail ground meat samples (chicken, pork, mixed beef/pork) purchased during a 5-month period, 10 <i>S. aureus</i> and 112 isolates of coagulase-negative staphylococcus (CoNS)/<i>Mammaliicoccus</i> comprising 20 species were recovered. <i>S. aureus</i> isolates were classified into five genetic types, i.e., <i>coa</i>-IIa/ST5, <i>coa</i>-VIc/ST352 (CC97), <i>coa</i>-VIIb/ST398, <i>coa</i>-Xa/ST15, and <i>coa</i>-XIc/ST9, which were all related to those of livestock-associated clones. All the staphylococcal isolates were <i>mecA</i>-negative and mostly susceptible to all the antimicrobials tested, except for ampicillin among <i>S. aureus</i> (resistance proportion; 50%). Among CoNS, the fosfomycin resistance gene <i>fosB</i> was prevalent (30/112; 26.8%), primarily in <i>S. capitis</i>, <i>S. warneri</i>, and <i>S. saprophyticus</i>. Phylogenetic analysis of <i>fosB</i> revealed the presence of seven clusters, showing broad diversity with 65–81% identity among different clusters. In the CoNS isolates from ground meat samples, <i>fosB</i> was assigned into three clusters, and <i>S. saprophyticus</i> harbored the most divergent <i>fosB</i> with three genetic groups. These findings suggested the circulation of multiple <i>fosB</i>-carrying plasmids among some CoNS species.https://www.mdpi.com/2076-0817/11/4/469retail meat<i>Staphylococcus aureus</i>coagulase-negative <i>Staphylococcus</i>multilocus sequence typing<i>fosB</i> |
| spellingShingle | Masako Osada Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Nobumichi Kobayashi Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i> Pathogens retail meat <i>Staphylococcus aureus</i> coagulase-negative <i>Staphylococcus</i> multilocus sequence typing <i>fosB</i> |
| title | Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i> |
| title_full | Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i> |
| title_fullStr | Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i> |
| title_full_unstemmed | Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i> |
| title_short | Prevalence and Antimicrobial Resistance of <i>Staphylococcus aureus</i> and Coagulase-Negative <i>Staphylococcus</i>/<i>Mammaliicoccus</i> from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene <i>fosB</i> |
| title_sort | prevalence and antimicrobial resistance of i staphylococcus aureus i and coagulase negative i staphylococcus i i mammaliicoccus i from retail ground meat identification of broad genetic diversity in fosfomycin resistance gene i fosb i |
| topic | retail meat <i>Staphylococcus aureus</i> coagulase-negative <i>Staphylococcus</i> multilocus sequence typing <i>fosB</i> |
| url | https://www.mdpi.com/2076-0817/11/4/469 |
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