Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites
The present study was carried out to identify the chemical composition of ethanol, flavonoid and anthocyanin extracts of Morus nigra fruits. The results revealed the presence of carbohydrates, glycosides, phenolic compounds and the absence of alkaloids, saponins, free amino acids, proteins and tanni...
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| Format: | Article |
| Language: | English |
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University of Zakho
2017-06-01
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| Series: | Science Journal of University of Zakho |
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| Online Access: | https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/495 |
| _version_ | 1818326021354553344 |
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| author | Najlaa K. Issa Rihan S. Abd-Aljabar |
| author_facet | Najlaa K. Issa Rihan S. Abd-Aljabar |
| author_sort | Najlaa K. Issa |
| collection | DOAJ |
| description | The present study was carried out to identify the chemical composition of ethanol, flavonoid and anthocyanin extracts of Morus nigra fruits. The results revealed the presence of carbohydrates, glycosides, phenolic compounds and the absence of alkaloids, saponins, free amino acids, proteins and tannins. TLC and column chromatography techniques were used to separate and purify the anthocyanin pigment from anthocyanin extract which is then subjected to acid hydrolysis to obtain the anthocyanidin pigment (a). Many techniques were also employed to confirm pigment (a) chemical structure including (TLC, UV-Visible and IR-Spectroscopy). According to the forgoing analytical results, the proposed structure for the isolated pigment (a) is cyanidin pigment. DNA nicking assay has been used to evaluate the ability of the prepared extracts and pigment (a) to prevent DNA nicking caused by free radicals generated from Fenton's reagent. The highest DNA protective effect was achieved in anthocyanin extract followed by pigment (a) and their effects were identical to the positive control (cyanidin chloride). Antibacterial activities of all extracts and pigment (a) were performed against the growth of two standards and pathogenic G+ and G- bacteria. The results indicated that all plants extracts showed more potent activity against standard bacteria strains than pathogenic ones. |
| first_indexed | 2024-12-13T11:53:45Z |
| format | Article |
| id | doaj.art-50b7f4285e4b495c9042a6766cd9c752 |
| institution | Directory Open Access Journal |
| issn | 2663-628X 2663-6298 |
| language | English |
| last_indexed | 2024-12-13T11:53:45Z |
| publishDate | 2017-06-01 |
| publisher | University of Zakho |
| record_format | Article |
| series | Science Journal of University of Zakho |
| spelling | doaj.art-50b7f4285e4b495c9042a6766cd9c7522022-12-21T23:47:16ZengUniversity of ZakhoScience Journal of University of Zakho2663-628X2663-62982017-06-015219820410.25271/2017.5.2.368495Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary MetabolitesNajlaa K. Issa0Rihan S. Abd-Aljabar1University of DuhokUniversity of ZakhoThe present study was carried out to identify the chemical composition of ethanol, flavonoid and anthocyanin extracts of Morus nigra fruits. The results revealed the presence of carbohydrates, glycosides, phenolic compounds and the absence of alkaloids, saponins, free amino acids, proteins and tannins. TLC and column chromatography techniques were used to separate and purify the anthocyanin pigment from anthocyanin extract which is then subjected to acid hydrolysis to obtain the anthocyanidin pigment (a). Many techniques were also employed to confirm pigment (a) chemical structure including (TLC, UV-Visible and IR-Spectroscopy). According to the forgoing analytical results, the proposed structure for the isolated pigment (a) is cyanidin pigment. DNA nicking assay has been used to evaluate the ability of the prepared extracts and pigment (a) to prevent DNA nicking caused by free radicals generated from Fenton's reagent. The highest DNA protective effect was achieved in anthocyanin extract followed by pigment (a) and their effects were identical to the positive control (cyanidin chloride). Antibacterial activities of all extracts and pigment (a) were performed against the growth of two standards and pathogenic G+ and G- bacteria. The results indicated that all plants extracts showed more potent activity against standard bacteria strains than pathogenic ones.https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/495Morus nigraFlavonoidAnthocyaninCyanidinDNA nickingAntibacterial activity |
| spellingShingle | Najlaa K. Issa Rihan S. Abd-Aljabar Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites Science Journal of University of Zakho Morus nigra Flavonoid Anthocyanin Cyanidin DNA nicking Antibacterial activity |
| title | Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites |
| title_full | Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites |
| title_fullStr | Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites |
| title_full_unstemmed | Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites |
| title_short | Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites |
| title_sort | phytochemical analysis antioxidant dna nicking assay and antibacterial activities of morus nigra l fruits secondary metabolites |
| topic | Morus nigra Flavonoid Anthocyanin Cyanidin DNA nicking Antibacterial activity |
| url | https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/495 |
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