Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging Mice
Senile osteoporosis is characterized by increased bone loss and fat accumulation in marrow. Curculigoside (CCG) is the major bioactive component of Curculigo orchioides, which has been used as anti-osteoporosis therapy for elder patients since antiquity. We aimed to investigate the underlying mechan...
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.767006/full |
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| author | Na Wang Na Wang Ziyi Li Ziyi Li Shilun Li Yukun Li Yukun Li Liu Gao Liu Gao Xiaoxue Bao Xiaoxue Bao Ke Wang Ke Wang Chang Liu Chang Liu Peng Xue Peng Xue Sijing Liu |
| author_facet | Na Wang Na Wang Ziyi Li Ziyi Li Shilun Li Yukun Li Yukun Li Liu Gao Liu Gao Xiaoxue Bao Xiaoxue Bao Ke Wang Ke Wang Chang Liu Chang Liu Peng Xue Peng Xue Sijing Liu |
| author_sort | Na Wang |
| collection | DOAJ |
| description | Senile osteoporosis is characterized by increased bone loss and fat accumulation in marrow. Curculigoside (CCG) is the major bioactive component of Curculigo orchioides, which has been used as anti-osteoporosis therapy for elder patients since antiquity. We aimed to investigate the underlying mechanisms by which CCG regulated the bone-fat balance in marrow of aging mice. In our study, CCG treatment was identified to interfere with the stem cell lineage commitment both in vivo and in vitro. In vivo, CCG promoted the transcriptional co-activator with PDZ-binding motif (TAZ) expression to reverse age-related bone loss and marrow adiposity. In vitro, proper concentration of CCG upregulated TAZ expression to increase osteogenesis and decrease adipogenesis of bone marrow mesenchymal stem cells (BMSCs). This regulating effect was discounted by TAZ knockdown or the use of MEK-ERK pathway inhibitor, UO126. Above all, our study confirmed the rescuing effects of CCG on the differential shift from adipogenesis to osteogenesis of BMSCs in aging mice and provided a scientific basis for the clinical use of CCG in senile osteoporosis. |
| first_indexed | 2024-12-14T08:58:23Z |
| format | Article |
| id | doaj.art-50b98c70a6c24a97b66e3aced27c1629 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-14T08:58:23Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-50b98c70a6c24a97b66e3aced27c16292022-12-21T23:08:52ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-12-01910.3389/fcell.2021.767006767006Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging MiceNa Wang0Na Wang1Ziyi Li2Ziyi Li3Shilun Li4Yukun Li5Yukun Li6Liu Gao7Liu Gao8Xiaoxue Bao9Xiaoxue Bao10Ke Wang11Ke Wang12Chang Liu13Chang Liu14Peng Xue15Peng Xue16Sijing Liu17Department of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaDepartment of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaDepartment of Joint Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaDepartment of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaDepartment of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaDepartment of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaDepartment of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaDepartment of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaKey Orthopaedic Biomechanics Laboratory of Hebei Province, Shijiazhuang, ChinaEditorial Department of Hebei Medical University, Hebei Medical University, Shijiazhuang, ChinaSenile osteoporosis is characterized by increased bone loss and fat accumulation in marrow. Curculigoside (CCG) is the major bioactive component of Curculigo orchioides, which has been used as anti-osteoporosis therapy for elder patients since antiquity. We aimed to investigate the underlying mechanisms by which CCG regulated the bone-fat balance in marrow of aging mice. In our study, CCG treatment was identified to interfere with the stem cell lineage commitment both in vivo and in vitro. In vivo, CCG promoted the transcriptional co-activator with PDZ-binding motif (TAZ) expression to reverse age-related bone loss and marrow adiposity. In vitro, proper concentration of CCG upregulated TAZ expression to increase osteogenesis and decrease adipogenesis of bone marrow mesenchymal stem cells (BMSCs). This regulating effect was discounted by TAZ knockdown or the use of MEK-ERK pathway inhibitor, UO126. Above all, our study confirmed the rescuing effects of CCG on the differential shift from adipogenesis to osteogenesis of BMSCs in aging mice and provided a scientific basis for the clinical use of CCG in senile osteoporosis.https://www.frontiersin.org/articles/10.3389/fcell.2021.767006/fullosteoporosiscurculigosideBMSCsTAZMEK-ERK pathway |
| spellingShingle | Na Wang Na Wang Ziyi Li Ziyi Li Shilun Li Yukun Li Yukun Li Liu Gao Liu Gao Xiaoxue Bao Xiaoxue Bao Ke Wang Ke Wang Chang Liu Chang Liu Peng Xue Peng Xue Sijing Liu Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging Mice Frontiers in Cell and Developmental Biology osteoporosis curculigoside BMSCs TAZ MEK-ERK pathway |
| title | Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging Mice |
| title_full | Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging Mice |
| title_fullStr | Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging Mice |
| title_full_unstemmed | Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging Mice |
| title_short | Curculigoside Ameliorates Bone Loss by Influencing Mesenchymal Stem Cell Fate in Aging Mice |
| title_sort | curculigoside ameliorates bone loss by influencing mesenchymal stem cell fate in aging mice |
| topic | osteoporosis curculigoside BMSCs TAZ MEK-ERK pathway |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.767006/full |
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