Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides
Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and a noxious weed prevalent in rice fields that remains challenging to farmers worldw...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-08-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514120301914 |
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| author | Lei Chen Gang Gu Chengxu Wang Zhufeng Chen Wei Yan Man Jin Gang Xie Junli Zhou Xing Wang Deng Xiaoyan Tang |
| author_facet | Lei Chen Gang Gu Chengxu Wang Zhufeng Chen Wei Yan Man Jin Gang Xie Junli Zhou Xing Wang Deng Xiaoyan Tang |
| author_sort | Lei Chen |
| collection | DOAJ |
| description | Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and a noxious weed prevalent in rice fields that remains challenging to farmers worldwide. Herbicide resistant plants can be obtained through transgenic approach or by mutagenesis of regular plant and screening of mutants with elevated resistance to herbicide. In this study, we conducted ethyl methyl sulfonate mutagenesis (EMS) to elite indica cultivar Huanghuazhan (HHZ) and screened for mutants resistant to imazapic, a herbicide that can inhibit the acetolactate synthase (ALS) in plants. We obtained three mutants of OsALS gene that have not been reported previously in rice. One of the mutants, with Trp548 changed to Met (W548M), was analyzed in more details in this study. This mutation had no negative effect on the plant physiology and morphology as well as rice yield. Compared with the imidazolinone-resistant mutant S627N (Ser627 changed to Asn) that has been deployed for Clearfield rice development, W548M mutant showed high levels of resistance to a broad spectrum of five families of ALS-inhibiting herbicides, in addition to a higher level of resistance to herbicides of the imidazolinone family. The herbicide-resistance was stably inherited by crossing into other rice lines. Thus, the W548M mutation provides a valuable resource for breeding of herbicide resistant rice and weed management. |
| first_indexed | 2024-12-16T12:29:51Z |
| format | Article |
| id | doaj.art-50bb3b1e133d4012a83763594263450e |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-16T12:29:51Z |
| publishDate | 2021-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-50bb3b1e133d4012a83763594263450e2022-12-21T22:31:43ZengKeAi Communications Co., Ltd.Crop Journal2214-51412021-08-0194750758Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicidesLei Chen0Gang Gu1Chengxu Wang2Zhufeng Chen3Wei Yan4Man Jin5Gang Xie6Junli Zhou7Xing Wang Deng8Xiaoyan Tang9Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, Guangdong, China; Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, China; Institute of Plant and Food Sciences, Department of Biology, Southern University of Science and Technology, Shenzhen 518055, Guangdong, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, Guangdong, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, ChinaShenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, China; Institute of Plant and Food Sciences, Department of Biology, Southern University of Science and Technology, Shenzhen 518055, Guangdong, China; Corresponding authors.Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, Guangdong, China; Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, Guangdong, China; Corresponding authors.Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and a noxious weed prevalent in rice fields that remains challenging to farmers worldwide. Herbicide resistant plants can be obtained through transgenic approach or by mutagenesis of regular plant and screening of mutants with elevated resistance to herbicide. In this study, we conducted ethyl methyl sulfonate mutagenesis (EMS) to elite indica cultivar Huanghuazhan (HHZ) and screened for mutants resistant to imazapic, a herbicide that can inhibit the acetolactate synthase (ALS) in plants. We obtained three mutants of OsALS gene that have not been reported previously in rice. One of the mutants, with Trp548 changed to Met (W548M), was analyzed in more details in this study. This mutation had no negative effect on the plant physiology and morphology as well as rice yield. Compared with the imidazolinone-resistant mutant S627N (Ser627 changed to Asn) that has been deployed for Clearfield rice development, W548M mutant showed high levels of resistance to a broad spectrum of five families of ALS-inhibiting herbicides, in addition to a higher level of resistance to herbicides of the imidazolinone family. The herbicide-resistance was stably inherited by crossing into other rice lines. Thus, the W548M mutation provides a valuable resource for breeding of herbicide resistant rice and weed management.http://www.sciencedirect.com/science/article/pii/S2214514120301914ALS-inhibiting herbicideHerbicide toleranceAcetolactate synthaseRiceMutant |
| spellingShingle | Lei Chen Gang Gu Chengxu Wang Zhufeng Chen Wei Yan Man Jin Gang Xie Junli Zhou Xing Wang Deng Xiaoyan Tang Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides Crop Journal ALS-inhibiting herbicide Herbicide tolerance Acetolactate synthase Rice Mutant |
| title | Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides |
| title_full | Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides |
| title_fullStr | Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides |
| title_full_unstemmed | Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides |
| title_short | Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides |
| title_sort | trp548met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of als inhibiting herbicides |
| topic | ALS-inhibiting herbicide Herbicide tolerance Acetolactate synthase Rice Mutant |
| url | http://www.sciencedirect.com/science/article/pii/S2214514120301914 |
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