Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) Mice
Purpose: To investigate the applicability of strip meniscometry tube (SMT) in the measurement of tear lactoferrin in non-obese diabetic mice (NOD). Methods: SMT (SMTube, Echo Electricity Co., Ltd., Tokyo, Japan) and fluorescein staining tests were performed on 7–14 week NOD- male mice (<i>n<...
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MDPI AG
2022-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/7/3700 |
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| author | Murat Dogru Takashi Kojima Taeko Nagata Kazuo Tsubota |
| author_facet | Murat Dogru Takashi Kojima Taeko Nagata Kazuo Tsubota |
| author_sort | Murat Dogru |
| collection | DOAJ |
| description | Purpose: To investigate the applicability of strip meniscometry tube (SMT) in the measurement of tear lactoferrin in non-obese diabetic mice (NOD). Methods: SMT (SMTube, Echo Electricity Co., Ltd., Tokyo, Japan) and fluorescein staining tests were performed on 7–14 week NOD- male mice (<i>n</i> = 4) and age and sex matched wild–type (Balbc, WT) mice (<i>n</i> = 5). Tears collected during SMT underwent lactoferrin concentration measurement by ELISA. Results: The mean SMT value was significantly lower in NOD mice compared to wild-type mice (<i>p</i> = 0.01). The mean corneal fluorescein staining score in the NOD mice was significantly lower compared with the wild-type mice (<i>p</i> = 0.03). The mean tear lactoferrin level also showed a significantly lower concentration in NOD mice (<i>p</i> = 0.02). Conclusions: SMT has been shown to be an effective tool in measuring tear volume in humans, cats, dogs, and mice. SMT may also serve as a useful tool for tear lactoferrin assessment in NOD and WT mice in experimental settings. |
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| id | doaj.art-50bdc039edf84418b2bedf22e67e64c7 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T12:04:39Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-50bdc039edf84418b2bedf22e67e64c72023-11-30T22:59:19ZengMDPI AGApplied Sciences2076-34172022-04-01127370010.3390/app12073700Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) MiceMurat Dogru0Takashi Kojima1Taeko Nagata2Kazuo Tsubota3Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8685, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8685, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8685, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo 160-8685, JapanPurpose: To investigate the applicability of strip meniscometry tube (SMT) in the measurement of tear lactoferrin in non-obese diabetic mice (NOD). Methods: SMT (SMTube, Echo Electricity Co., Ltd., Tokyo, Japan) and fluorescein staining tests were performed on 7–14 week NOD- male mice (<i>n</i> = 4) and age and sex matched wild–type (Balbc, WT) mice (<i>n</i> = 5). Tears collected during SMT underwent lactoferrin concentration measurement by ELISA. Results: The mean SMT value was significantly lower in NOD mice compared to wild-type mice (<i>p</i> = 0.01). The mean corneal fluorescein staining score in the NOD mice was significantly lower compared with the wild-type mice (<i>p</i> = 0.03). The mean tear lactoferrin level also showed a significantly lower concentration in NOD mice (<i>p</i> = 0.02). Conclusions: SMT has been shown to be an effective tool in measuring tear volume in humans, cats, dogs, and mice. SMT may also serve as a useful tool for tear lactoferrin assessment in NOD and WT mice in experimental settings.https://www.mdpi.com/2076-3417/12/7/3700non-obese diabetic micedry eyestrip meniscometrytear lactoferrin |
| spellingShingle | Murat Dogru Takashi Kojima Taeko Nagata Kazuo Tsubota Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) Mice Applied Sciences non-obese diabetic mice dry eye strip meniscometry tear lactoferrin |
| title | Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) Mice |
| title_full | Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) Mice |
| title_fullStr | Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) Mice |
| title_full_unstemmed | Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) Mice |
| title_short | Strip Meniscometry Tube in the Assessment of Tear Lactoferrin in Non-Obese Diabetic (NOD) Mice |
| title_sort | strip meniscometry tube in the assessment of tear lactoferrin in non obese diabetic nod mice |
| topic | non-obese diabetic mice dry eye strip meniscometry tear lactoferrin |
| url | https://www.mdpi.com/2076-3417/12/7/3700 |
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