Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease
Chronic inflammation in inflammatory bowel disease (IBD) triggers significant extracellular matrix remodeling, including elastin remodeling, leading to severe clinical complications. Novel methods to assess intestinal tissue destruction may act as surrogate markers of endoscopic disease activity, re...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/13/1/21 |
| _version_ | 1797358589198729216 |
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| author | Martin Pehrsson Viktor Domislovic Marta Sorokina Alexdottir Marko Brinar Morten Asser Karsdal Ana Barisic Zeljko Krznaric Joachim Høg Mortensen |
| author_facet | Martin Pehrsson Viktor Domislovic Marta Sorokina Alexdottir Marko Brinar Morten Asser Karsdal Ana Barisic Zeljko Krznaric Joachim Høg Mortensen |
| author_sort | Martin Pehrsson |
| collection | DOAJ |
| description | Chronic inflammation in inflammatory bowel disease (IBD) triggers significant extracellular matrix remodeling, including elastin remodeling, leading to severe clinical complications. Novel methods to assess intestinal tissue destruction may act as surrogate markers of endoscopic disease activity, relieving patients of invasive endoscopy. We explored the noninvasive blood-based biomarkers ELP-3 and ELM-12, measuring elastin degradation in IBD. In a study involving 104 Crohn’s disease (CD), 39 ulcerative colitis (UC), and 29 healthy donors, we assessed these biomarkers’ association with endoscopic and clinical disease activity using ELISA. Patients were evaluated based on the SES-CD and CDAI for CD patients and modified MES and partial Mayo for UC patients. ELP-3 and ELM-12 were elevated in patients with IBD. Discerning CD patients in endoscopic remission and mild from moderate to severe, ELP-3 provided an AUC of 0.69 and ELM-12 an AUC of 0.73. The ELP-3 biomarker was associated with UC patients and provided the highest diagnostic power of 0.87 for remission vs. active clinical disease. The data suggest an association of ELP-3 with active CD and ELM-12 with endoscopic remission in CD patients. Additionally, ELP-3 could identify UC patients with active clinical disease from patients in remission. The noninvasive biomarkers ELP-3 and ELM-12 could be potential surrogate biomarkers of elastin degradation and endoscopic and clinical disease markers. |
| first_indexed | 2024-03-08T15:04:06Z |
| format | Article |
| id | doaj.art-50bf71ee4cfd4c68bd456950328bf450 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-08T15:04:06Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-50bf71ee4cfd4c68bd456950328bf4502024-01-10T15:00:40ZengMDPI AGJournal of Clinical Medicine2077-03832023-12-011312110.3390/jcm13010021Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel DiseaseMartin Pehrsson0Viktor Domislovic1Marta Sorokina Alexdottir2Marko Brinar3Morten Asser Karsdal4Ana Barisic5Zeljko Krznaric6Joachim Høg Mortensen7Biomarkers and Research, Nordic Bioscience A/S, 2730 Herlev, DenmarkDepartment of Gastroenterology and Hepatology, University Hospital Center Zagreb, 10000 Zagreb, CroatiaBiomarkers and Research, Nordic Bioscience A/S, 2730 Herlev, DenmarkDepartment of Gastroenterology and Hepatology, University Hospital Center Zagreb, 10000 Zagreb, CroatiaBiomarkers and Research, Nordic Bioscience A/S, 2730 Herlev, DenmarkCenter for Clinical Nutrition, University Hospital Centre Zagreb, 10000 Zagreb, CroatiaDepartment of Gastroenterology and Hepatology, University Hospital Center Zagreb, 10000 Zagreb, CroatiaBiomarkers and Research, Nordic Bioscience A/S, 2730 Herlev, DenmarkChronic inflammation in inflammatory bowel disease (IBD) triggers significant extracellular matrix remodeling, including elastin remodeling, leading to severe clinical complications. Novel methods to assess intestinal tissue destruction may act as surrogate markers of endoscopic disease activity, relieving patients of invasive endoscopy. We explored the noninvasive blood-based biomarkers ELP-3 and ELM-12, measuring elastin degradation in IBD. In a study involving 104 Crohn’s disease (CD), 39 ulcerative colitis (UC), and 29 healthy donors, we assessed these biomarkers’ association with endoscopic and clinical disease activity using ELISA. Patients were evaluated based on the SES-CD and CDAI for CD patients and modified MES and partial Mayo for UC patients. ELP-3 and ELM-12 were elevated in patients with IBD. Discerning CD patients in endoscopic remission and mild from moderate to severe, ELP-3 provided an AUC of 0.69 and ELM-12 an AUC of 0.73. The ELP-3 biomarker was associated with UC patients and provided the highest diagnostic power of 0.87 for remission vs. active clinical disease. The data suggest an association of ELP-3 with active CD and ELM-12 with endoscopic remission in CD patients. Additionally, ELP-3 could identify UC patients with active clinical disease from patients in remission. The noninvasive biomarkers ELP-3 and ELM-12 could be potential surrogate biomarkers of elastin degradation and endoscopic and clinical disease markers.https://www.mdpi.com/2077-0383/13/1/21biomarkerIBDelastin remodelingCrohn’s diseaseulcerative colitisneoepitope |
| spellingShingle | Martin Pehrsson Viktor Domislovic Marta Sorokina Alexdottir Marko Brinar Morten Asser Karsdal Ana Barisic Zeljko Krznaric Joachim Høg Mortensen Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease Journal of Clinical Medicine biomarker IBD elastin remodeling Crohn’s disease ulcerative colitis neoepitope |
| title | Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease |
| title_full | Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease |
| title_fullStr | Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease |
| title_full_unstemmed | Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease |
| title_short | Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease |
| title_sort | blood based biomarkers reflecting protease 3 and mmp 12 catalyzed elastin degradation as potential noninvasive surrogate markers of endoscopic and clinical disease in inflammatory bowel disease |
| topic | biomarker IBD elastin remodeling Crohn’s disease ulcerative colitis neoepitope |
| url | https://www.mdpi.com/2077-0383/13/1/21 |
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