Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing
The development of optical biosensors based on structural colors generated by short-range ordered colloidal particles is attracting growing interest due to their non-iridescent and non-fading features. In this study, a biomimetic approach using biopolymers for the various steps of sensor constructio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137023000079 |
| _version_ | 1797868279163781120 |
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| author | Akmaral Suleimenova Manuela F. Frasco Francisco A.G. Soares da Silva Miguel Gama Elvira Fortunato M. Goreti F. Sales |
| author_facet | Akmaral Suleimenova Manuela F. Frasco Francisco A.G. Soares da Silva Miguel Gama Elvira Fortunato M. Goreti F. Sales |
| author_sort | Akmaral Suleimenova |
| collection | DOAJ |
| description | The development of optical biosensors based on structural colors generated by short-range ordered colloidal particles is attracting growing interest due to their non-iridescent and non-fading features. In this study, a biomimetic approach using biopolymers for the various steps of sensor construction is presented. Bacterial nanocellulose (BNC) has many foreseen applications in biomedical engineering because of its biocompatibility, good mechanical strength, and large modifiable surface area. Herein, a novel approach is taken by using functionalized BNC as a substrate to build a molecularly imprinted photonic sensing layer. BNC was modified with polydopamine (PDA), which improved the adhesion and mechanical properties of the BNC substrate while providing simultaneously a black background for color saturation. A molecularly imprinted polymer (MIP) also made of PDA was used to create the recognition sites for the biomarker lysozyme. A monodisperse colloidal suspension of silica particles was first synthesized and used as core of the MIP shell, and then the photonic structure was assembled on the PDA-BNC membrane. The biosensor showed a detection limit of about 0.8 nmol L−1 of lysozyme in spiked human serum and demonstrated to be selective against cystatin C. These properties, combined with biocompatible, eco-friendly, and low-cost materials, offer a sustainable sensing platform with great potential for healthcare applications. |
| first_indexed | 2024-04-09T23:53:23Z |
| format | Article |
| id | doaj.art-50c1396c28be4c41b2506dd952bbf638 |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-09T23:53:23Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-50c1396c28be4c41b2506dd952bbf6382023-03-17T04:33:52ZengElsevierBiosensors and Bioelectronics: X2590-13702023-05-0113100310Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensingAkmaral Suleimenova0Manuela F. Frasco1Francisco A.G. Soares da Silva2Miguel Gama3Elvira Fortunato4M. Goreti F. Sales5BioMark@ISEP/CEB – LABBELS, School of Engineering, Polytechnic Institute of Porto, Porto, Portugal; BioMark@UC/CEB – LABBELS, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal; CENIMAT|i3N, Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP/UNINOVA, Caparica, PortugalBioMark@UC/CEB – LABBELS, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal; Corresponding author. BioMark@UC/CEB – LABBELS, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Coimbra, PortugalCEB – LABBELS, Centre of Biological Engineering, University of Minho, Braga, PortugalCEB – LABBELS, Centre of Biological Engineering, University of Minho, Braga, PortugalCENIMAT|i3N, Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP/UNINOVA, Caparica, PortugalBioMark@ISEP/CEB – LABBELS, School of Engineering, Polytechnic Institute of Porto, Porto, Portugal; BioMark@UC/CEB – LABBELS, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal; Corresponding author. BioMark@UC/CEB – LABBELS, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Coimbra, PortugalThe development of optical biosensors based on structural colors generated by short-range ordered colloidal particles is attracting growing interest due to their non-iridescent and non-fading features. In this study, a biomimetic approach using biopolymers for the various steps of sensor construction is presented. Bacterial nanocellulose (BNC) has many foreseen applications in biomedical engineering because of its biocompatibility, good mechanical strength, and large modifiable surface area. Herein, a novel approach is taken by using functionalized BNC as a substrate to build a molecularly imprinted photonic sensing layer. BNC was modified with polydopamine (PDA), which improved the adhesion and mechanical properties of the BNC substrate while providing simultaneously a black background for color saturation. A molecularly imprinted polymer (MIP) also made of PDA was used to create the recognition sites for the biomarker lysozyme. A monodisperse colloidal suspension of silica particles was first synthesized and used as core of the MIP shell, and then the photonic structure was assembled on the PDA-BNC membrane. The biosensor showed a detection limit of about 0.8 nmol L−1 of lysozyme in spiked human serum and demonstrated to be selective against cystatin C. These properties, combined with biocompatible, eco-friendly, and low-cost materials, offer a sustainable sensing platform with great potential for healthcare applications.http://www.sciencedirect.com/science/article/pii/S2590137023000079Bacterial nanocellulosePolydopamineMolecular imprinting technologyPhotonic polymersLysozyme |
| spellingShingle | Akmaral Suleimenova Manuela F. Frasco Francisco A.G. Soares da Silva Miguel Gama Elvira Fortunato M. Goreti F. Sales Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing Biosensors and Bioelectronics: X Bacterial nanocellulose Polydopamine Molecular imprinting technology Photonic polymers Lysozyme |
| title | Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
| title_full | Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
| title_fullStr | Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
| title_full_unstemmed | Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
| title_short | Bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials: Application to lysozyme sensing |
| title_sort | bacterial nanocellulose membrane as novel substrate for biomimetic structural color materials application to lysozyme sensing |
| topic | Bacterial nanocellulose Polydopamine Molecular imprinting technology Photonic polymers Lysozyme |
| url | http://www.sciencedirect.com/science/article/pii/S2590137023000079 |
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