Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines
Abstract Background Breast cancer is one of the most common diseases worldwide that affects women of reproductive age. miR-221 and miR-222 are two highly homogeneous microRNAs that play pivotal roles in many cellular processes and regulate the Wnt/β-catenin signaling pathway. Curcumin (CUR), a yello...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-02-01
|
| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13000-024-01468-3 |
| _version_ | 1797276056058593280 |
|---|---|
| author | Touba Eslaminejad Seyed Noureddin Nematollahi-Mahani Marzieh Lotfian Sargazi Mehdi Ansari Vida Mirzaie |
| author_facet | Touba Eslaminejad Seyed Noureddin Nematollahi-Mahani Marzieh Lotfian Sargazi Mehdi Ansari Vida Mirzaie |
| author_sort | Touba Eslaminejad |
| collection | DOAJ |
| description | Abstract Background Breast cancer is one of the most common diseases worldwide that affects women of reproductive age. miR-221 and miR-222 are two highly homogeneous microRNAs that play pivotal roles in many cellular processes and regulate the Wnt/β-catenin signaling pathway. Curcumin (CUR), a yellow polyphenolic compound, targets numerous signaling pathways relevant to cancer therapy. The main aim of this study was to compare the ability of chitosan curcumin nanoparticle (CC-CUR) formulation with the curcumin in modulating miR-221 and miR-222 expression through Wnt/β-catenin signaling pathway in MCF-7, MDA-MB-231 and SK-BR-3 breast cancer cell lines. Method Chitosan-cyclodextrin-tripolyphosphate containing curcumin nanoparticles (CC-CUR) were prepared. Cytotoxicity of the CUR and CC-CUR was evaluated. Experimental groups including CC-CUR, CUR and negative control were designed. The expression of miR-221 and miR-222 and Wnt/β-catenin pathway genes was measured. Results The level of miR-221 and miR-222 and β-catenin genes decreased in MCF-7 and MDA-MB-231 cells and WIF1 gene increased in all cells in CC-CUR group. However, the results in SK-BR-3 cell line were unexpected; since miRs and WIF1 gene expressions were increased following CC-CUR administration and β-catenin decreased by administration of CUR. Conclusion Although the composite form of curcumin decreased the expression of miR-221 and miR-222 in MCF-7 and MDA cells, with significant decreasing of β-catenin and increasing of WIF1 gene in almost all three cell lines, we can conclude than this formulation exerts its effect mainly through the Wnt/β-catenin pathway. These preliminary findings may pave the way for the use of curcumin nanoparticles in the treatment of some known cancers. |
| first_indexed | 2024-03-07T15:22:50Z |
| format | Article |
| id | doaj.art-50c69e5a21a94a5cb96c50425f955f9d |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-03-07T15:22:50Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-50c69e5a21a94a5cb96c50425f955f9d2024-03-05T17:30:28ZengBMCDiagnostic Pathology1746-15962024-02-011911910.1186/s13000-024-01468-3Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell linesTouba Eslaminejad0Seyed Noureddin Nematollahi-Mahani1Marzieh Lotfian Sargazi2Mehdi Ansari3Vida Mirzaie4Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical SciencesDepartment of Anatomy, Afzalipour School of Medicine, Kerman University of Medical SciencesPhysiology Research Center, Institute of Neuropharmacology, Kerman University of Medical SciencesDepartments of Drug and Food Control, Faculty of Pharmacy, Kerman University of Medical SciencesDepartment of Anatomy, Afzalipour School of Medicine, Kerman University of Medical SciencesAbstract Background Breast cancer is one of the most common diseases worldwide that affects women of reproductive age. miR-221 and miR-222 are two highly homogeneous microRNAs that play pivotal roles in many cellular processes and regulate the Wnt/β-catenin signaling pathway. Curcumin (CUR), a yellow polyphenolic compound, targets numerous signaling pathways relevant to cancer therapy. The main aim of this study was to compare the ability of chitosan curcumin nanoparticle (CC-CUR) formulation with the curcumin in modulating miR-221 and miR-222 expression through Wnt/β-catenin signaling pathway in MCF-7, MDA-MB-231 and SK-BR-3 breast cancer cell lines. Method Chitosan-cyclodextrin-tripolyphosphate containing curcumin nanoparticles (CC-CUR) were prepared. Cytotoxicity of the CUR and CC-CUR was evaluated. Experimental groups including CC-CUR, CUR and negative control were designed. The expression of miR-221 and miR-222 and Wnt/β-catenin pathway genes was measured. Results The level of miR-221 and miR-222 and β-catenin genes decreased in MCF-7 and MDA-MB-231 cells and WIF1 gene increased in all cells in CC-CUR group. However, the results in SK-BR-3 cell line were unexpected; since miRs and WIF1 gene expressions were increased following CC-CUR administration and β-catenin decreased by administration of CUR. Conclusion Although the composite form of curcumin decreased the expression of miR-221 and miR-222 in MCF-7 and MDA cells, with significant decreasing of β-catenin and increasing of WIF1 gene in almost all three cell lines, we can conclude than this formulation exerts its effect mainly through the Wnt/β-catenin pathway. These preliminary findings may pave the way for the use of curcumin nanoparticles in the treatment of some known cancers.https://doi.org/10.1186/s13000-024-01468-3Breast cancermicro RNAsmiR-221miR-222Wnt/β-catenin |
| spellingShingle | Touba Eslaminejad Seyed Noureddin Nematollahi-Mahani Marzieh Lotfian Sargazi Mehdi Ansari Vida Mirzaie Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines Diagnostic Pathology Breast cancer micro RNAs miR-221 miR-222 Wnt/β-catenin |
| title | Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines |
| title_full | Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines |
| title_fullStr | Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines |
| title_full_unstemmed | Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines |
| title_short | Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines |
| title_sort | evaluating the effects of curcumin nano chitosan on mir 221 and mir 222 expression and wnt β catenin pathways in mcf 7 mda mb 231 and skbr3 cell lines |
| topic | Breast cancer micro RNAs miR-221 miR-222 Wnt/β-catenin |
| url | https://doi.org/10.1186/s13000-024-01468-3 |
| work_keys_str_mv | AT toubaeslaminejad evaluatingtheeffectsofcurcuminnanochitosanonmir221andmir222expressionandwntbcateninpathwaysinmcf7mdamb231andskbr3celllines AT seyednoureddinnematollahimahani evaluatingtheeffectsofcurcuminnanochitosanonmir221andmir222expressionandwntbcateninpathwaysinmcf7mdamb231andskbr3celllines AT marziehlotfiansargazi evaluatingtheeffectsofcurcuminnanochitosanonmir221andmir222expressionandwntbcateninpathwaysinmcf7mdamb231andskbr3celllines AT mehdiansari evaluatingtheeffectsofcurcuminnanochitosanonmir221andmir222expressionandwntbcateninpathwaysinmcf7mdamb231andskbr3celllines AT vidamirzaie evaluatingtheeffectsofcurcuminnanochitosanonmir221andmir222expressionandwntbcateninpathwaysinmcf7mdamb231andskbr3celllines |