Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41
Microalgae are recognized as a promising and valuable source of lutein. However, the current two-stage method for lutein production has drawbacks, such as complex operations and a long cultivation time. Additionally, utilizing heterotrophic fermentation to cultivate microalgae for lutein production...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/8/768 |
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| author | Yunlei Fu Lanbo Yi Shufang Yang Xue Lu Bin Liu Feng Chen Junchao Huang Kawing Cheng Han Sun Xiaolei Wu |
| author_facet | Yunlei Fu Lanbo Yi Shufang Yang Xue Lu Bin Liu Feng Chen Junchao Huang Kawing Cheng Han Sun Xiaolei Wu |
| author_sort | Yunlei Fu |
| collection | DOAJ |
| description | Microalgae are recognized as a promising and valuable source of lutein. However, the current two-stage method for lutein production has drawbacks, such as complex operations and a long cultivation time. Additionally, utilizing heterotrophic fermentation to cultivate microalgae for lutein production leads to low lutein content due to the absence of light. In this study, we proposed a novel cultivation method that involves light induction of the seed culture to enhance lutein production during the heterotrophic cultivation phase of <i>Chlorella protothecoides</i> CS-41. To gain comprehensive insights into the underlying mechanisms of this method, we conducted qualitative and quantitative analyses of specific metabolites related to central carbon metabolism. The results revealed that low-light induction of seeds exhibited higher carbon efficiency compared to cells continuously subjected to heterotrophic cultivation, which may explain the observed increase in biomass and lutein content in cultures. Cultures after low-light induction of seed exhibited significantly higher lutein content (2.71 mg/g), yield (66.49 mg/L) and productivity (8.59 mg/L/d) compared to those consistently cultivated under heterotrophic conditions (2.37 mg/g, 37.45 mg/L, 4.68 mg/L/d). This cultivation strategy effectively enhances lutein yields, reduces production costs and holds the potential for broader application in other algal species for pigment production. |
| first_indexed | 2024-03-10T23:57:11Z |
| format | Article |
| id | doaj.art-50c6aadf8eec462c8f0a0baf2fba9953 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T23:57:11Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-50c6aadf8eec462c8f0a0baf2fba99532023-11-19T01:02:37ZengMDPI AGFermentation2311-56372023-08-019876810.3390/fermentation9080768Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41Yunlei Fu0Lanbo Yi1Shufang Yang2Xue Lu3Bin Liu4Feng Chen5Junchao Huang6Kawing Cheng7Han Sun8Xiaolei Wu9Institute of Ocean Research, College of Engineering, Peking University, Beijing 100871, ChinaInstitute of Ocean Research, College of Engineering, Peking University, Beijing 100871, ChinaShenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaInstitute of New Materials and Advanced Manufacturing, Beijing Academy of Science and Technology, Beijing 100089, ChinaShenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaShenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaShenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaShenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaShenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaInstitute of Ocean Research, College of Engineering, Peking University, Beijing 100871, ChinaMicroalgae are recognized as a promising and valuable source of lutein. However, the current two-stage method for lutein production has drawbacks, such as complex operations and a long cultivation time. Additionally, utilizing heterotrophic fermentation to cultivate microalgae for lutein production leads to low lutein content due to the absence of light. In this study, we proposed a novel cultivation method that involves light induction of the seed culture to enhance lutein production during the heterotrophic cultivation phase of <i>Chlorella protothecoides</i> CS-41. To gain comprehensive insights into the underlying mechanisms of this method, we conducted qualitative and quantitative analyses of specific metabolites related to central carbon metabolism. The results revealed that low-light induction of seeds exhibited higher carbon efficiency compared to cells continuously subjected to heterotrophic cultivation, which may explain the observed increase in biomass and lutein content in cultures. Cultures after low-light induction of seed exhibited significantly higher lutein content (2.71 mg/g), yield (66.49 mg/L) and productivity (8.59 mg/L/d) compared to those consistently cultivated under heterotrophic conditions (2.37 mg/g, 37.45 mg/L, 4.68 mg/L/d). This cultivation strategy effectively enhances lutein yields, reduces production costs and holds the potential for broader application in other algal species for pigment production.https://www.mdpi.com/2311-5637/9/8/768microalgaeheterotrophic culturelutein biosynthesismetabolic pathway |
| spellingShingle | Yunlei Fu Lanbo Yi Shufang Yang Xue Lu Bin Liu Feng Chen Junchao Huang Kawing Cheng Han Sun Xiaolei Wu Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41 Fermentation microalgae heterotrophic culture lutein biosynthesis metabolic pathway |
| title | Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41 |
| title_full | Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41 |
| title_fullStr | Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41 |
| title_full_unstemmed | Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41 |
| title_short | Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of <i>Chlorella protothecoides</i> CS-41 |
| title_sort | light induction of seed culture accelerates lutein accumulation in heterotrophic fermentation of i chlorella protothecoides i cs 41 |
| topic | microalgae heterotrophic culture lutein biosynthesis metabolic pathway |
| url | https://www.mdpi.com/2311-5637/9/8/768 |
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