Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco)
Thrombolytic agents are used for thrombolytic therapy to dissolve blood clots that form in a blood vessel. All currently used thrombolytic agents have unfavorable shortcomings, such as gastrointestinal bleeding, allergic reactions, and thrombolytic agent resistance, treatment for some of which can b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2023-12-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/82390 |
| _version_ | 1797367824055795712 |
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| author | Urbanus Yustus Lebuan Roga Florida Kembaren Merry Meryam Martgrita Cut Rizlani Kholibrina |
| author_facet | Urbanus Yustus Lebuan Roga Florida Kembaren Merry Meryam Martgrita Cut Rizlani Kholibrina |
| author_sort | Urbanus Yustus Lebuan |
| collection | DOAJ |
| description | Thrombolytic agents are used for thrombolytic therapy to dissolve blood clots that form in a blood vessel. All currently used thrombolytic agents have unfavorable shortcomings, such as gastrointestinal bleeding, allergic reactions, and thrombolytic agent resistance, treatment for some of which can be quite expensive. As a result, the search for thrombolytic agents derived from plants is currently taking place. Some plants have been discovered to contain protease enzymes with thrombolytic activity; pharmaceuticals derived from plants are believed to be safer. Jernang rattan (Daemonorops draco) is a plant of the Arecaceae family and is known to produce resin. Jernang rattan resin is also known to have antioxidant, antiseptic, antitumor, antimicrobial, and cytotoxic activity, but very limited information on proteolytic activity of the protease from this plant. This research aims to isolate proteases from the leaves and fruit flesh of the rattan jernang plant (D. draco) and to investigate the proteolytic activity of the isolated proteases. The protease was isolated from the leaves and the fruit flesh, and then partially purified by ammonium sulfate precipitation. The radial caseinolytic assay showed that protease in a 60% ammonium sulfate fraction gave a clear zone, with diameters of 1.4 cm and 1.8 cm for the protease isolated from leaves and fruit flesh, respectively. A Folin‐Ciocalteau assay showed that the enzymes isolated were able to hydrolyze casein and release L‐tyrosine, with activity of 0.158 U/mL and 0.174 U/mL for the protease from the leaves and fruit flesh, respectively. A fibrinogenolytic assay showed that the protease from the fruit flesh hydrolyzed the A‐α, B‐β and the γ chain of human fibrinogen, while the protease from the leaves hydrolyzed the A‐α and γ chain. Both proteases were inhibited by 56% by phenylmethylsulfonyl fluoride (PMSF), indicating that the enzymes are serine proteases. Based on the assay results obtained, it can be concluded that proteases isolated from the leaves and fruit flesh have potential as thrombolytic proteases. |
| first_indexed | 2024-03-08T17:23:37Z |
| format | Article |
| id | doaj.art-50c965257152460f96de16022079b088 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-03-08T17:23:37Z |
| publishDate | 2023-12-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-50c965257152460f96de16022079b0882024-01-03T02:56:00ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412023-12-0128424825310.22146/ijbiotech.8239034804Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco)Urbanus Yustus Lebuan0Roga Florida Kembaren1Merry Meryam Martgrita2Cut Rizlani Kholibrina3Bioprocess Engineering Study Program, Faculty of Biotechnology, Institut Teknologi Del, Jalan Sisingamangaraja, Laguboti, Toba 22381, North Sumatera, IndonesiaBioprocess Engineering Study Program, Faculty of Biotechnology, Institut Teknologi Del, Jalan Sisingamangaraja, Laguboti, Toba 22381, North Sumatera, IndonesiaBioprocess Engineering Study Program, Faculty of Biotechnology, Institut Teknologi Del, Jalan Sisingamangaraja, Laguboti, Toba 22381, North Sumatera, IndonesiaPusat Riset Biomassa dan Bioproduk BRIN Kawasan Sains dan Teknologi Dr.(H.C.) Ir.H. Soekarno, Jl.Raya Bogor Km. 46 Cibinong 16911, IndonesiaThrombolytic agents are used for thrombolytic therapy to dissolve blood clots that form in a blood vessel. All currently used thrombolytic agents have unfavorable shortcomings, such as gastrointestinal bleeding, allergic reactions, and thrombolytic agent resistance, treatment for some of which can be quite expensive. As a result, the search for thrombolytic agents derived from plants is currently taking place. Some plants have been discovered to contain protease enzymes with thrombolytic activity; pharmaceuticals derived from plants are believed to be safer. Jernang rattan (Daemonorops draco) is a plant of the Arecaceae family and is known to produce resin. Jernang rattan resin is also known to have antioxidant, antiseptic, antitumor, antimicrobial, and cytotoxic activity, but very limited information on proteolytic activity of the protease from this plant. This research aims to isolate proteases from the leaves and fruit flesh of the rattan jernang plant (D. draco) and to investigate the proteolytic activity of the isolated proteases. The protease was isolated from the leaves and the fruit flesh, and then partially purified by ammonium sulfate precipitation. The radial caseinolytic assay showed that protease in a 60% ammonium sulfate fraction gave a clear zone, with diameters of 1.4 cm and 1.8 cm for the protease isolated from leaves and fruit flesh, respectively. A Folin‐Ciocalteau assay showed that the enzymes isolated were able to hydrolyze casein and release L‐tyrosine, with activity of 0.158 U/mL and 0.174 U/mL for the protease from the leaves and fruit flesh, respectively. A fibrinogenolytic assay showed that the protease from the fruit flesh hydrolyzed the A‐α, B‐β and the γ chain of human fibrinogen, while the protease from the leaves hydrolyzed the A‐α and γ chain. Both proteases were inhibited by 56% by phenylmethylsulfonyl fluoride (PMSF), indicating that the enzymes are serine proteases. Based on the assay results obtained, it can be concluded that proteases isolated from the leaves and fruit flesh have potential as thrombolytic proteases.https://jurnal.ugm.ac.id/ijbiotech/article/view/82390daemonorops dracofibrinogenolyticproteaseserine proteasethrombolytic |
| spellingShingle | Urbanus Yustus Lebuan Roga Florida Kembaren Merry Meryam Martgrita Cut Rizlani Kholibrina Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco) Indonesian Journal of Biotechnology daemonorops draco fibrinogenolytic protease serine protease thrombolytic |
| title | Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco) |
| title_full | Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco) |
| title_fullStr | Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco) |
| title_full_unstemmed | Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco) |
| title_short | Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco) |
| title_sort | thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant daemonorops draco |
| topic | daemonorops draco fibrinogenolytic protease serine protease thrombolytic |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/82390 |
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