Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the Spinal Cord of Rats with Neuropathic Pain
Background and purpose: Inflammation plays an important role in the development of neuropathic pain (NP). Considering the anti-inflammatory effects of mesenchymal stem cells (MSCs) and their conditioned medium (CM), we aimed to investigate the effect of MSC-CM on the expression of CCL3 and CCL4 gene...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2023-11-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-19756-en.pdf |
| _version_ | 1797561643633213440 |
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| author | Mohammad-Shafi Mojadadi Kimia Shahrazad Samad Nazemi Saeideh Sadat Shobeiri |
| author_facet | Mohammad-Shafi Mojadadi Kimia Shahrazad Samad Nazemi Saeideh Sadat Shobeiri |
| author_sort | Mohammad-Shafi Mojadadi |
| collection | DOAJ |
| description | Background and purpose: Inflammation plays an important role in the development of neuropathic pain (NP). Considering the anti-inflammatory effects of mesenchymal stem cells (MSCs) and their conditioned medium (CM), we aimed to investigate the effect of MSC-CM on the expression of CCL3 and CCL4 genes in the spinal cord of rats with NP.
Materials and methods: In this experimental-interventional study, 28 male Wistar rats weighing 200-300 grams were included. NP was induced in male rats using the chronic constriction injury (CCI) model of the sciatic nerve. MSCs-CM was injected intraperitoneally (1ml) into each rat in the experimental group one day before surgery (-1), and 7 and 11 days after the surgery, while the rats of control group received DMEM. At the end of the study (day 15), the expression level of CCL3 and CCL4 genes in the animals’ spinal cord was measured using Real time PCR.
Results: Molecular studies showed a significant decrease in CCL3 gene expression (P<0.05) in CM treated animals compared to the control group, but in term of CCL4 gene expression, there was no significant difference between the groups.
Conclusion: Anti-NP effects of MSCs-CM seem to be partly mediated through downregulation of CCL3 chemokine in the spinal cord. |
| first_indexed | 2024-03-10T18:17:49Z |
| format | Article |
| id | doaj.art-50cff5bb945f4574a35345b81bf9c402 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-03-10T18:17:49Z |
| publishDate | 2023-11-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-50cff5bb945f4574a35345b81bf9c4022023-11-20T07:33:34ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792023-11-0133226164171Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the Spinal Cord of Rats with Neuropathic PainMohammad-Shafi Mojadadi0Kimia Shahrazad1Samad Nazemi2Saeideh Sadat Shobeiri3 Associate Professor, Department of Immunology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran Medical Student, Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran Associate Professor, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran PhD in Immunology, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran Background and purpose: Inflammation plays an important role in the development of neuropathic pain (NP). Considering the anti-inflammatory effects of mesenchymal stem cells (MSCs) and their conditioned medium (CM), we aimed to investigate the effect of MSC-CM on the expression of CCL3 and CCL4 genes in the spinal cord of rats with NP. Materials and methods: In this experimental-interventional study, 28 male Wistar rats weighing 200-300 grams were included. NP was induced in male rats using the chronic constriction injury (CCI) model of the sciatic nerve. MSCs-CM was injected intraperitoneally (1ml) into each rat in the experimental group one day before surgery (-1), and 7 and 11 days after the surgery, while the rats of control group received DMEM. At the end of the study (day 15), the expression level of CCL3 and CCL4 genes in the animals’ spinal cord was measured using Real time PCR. Results: Molecular studies showed a significant decrease in CCL3 gene expression (P<0.05) in CM treated animals compared to the control group, but in term of CCL4 gene expression, there was no significant difference between the groups. Conclusion: Anti-NP effects of MSCs-CM seem to be partly mediated through downregulation of CCL3 chemokine in the spinal cord.http://jmums.mazums.ac.ir/article-1-19756-en.pdfneuropathic painmesenchymal stem cellconditioned mediumccl3ccl4hyperalgesiaallodynia |
| spellingShingle | Mohammad-Shafi Mojadadi Kimia Shahrazad Samad Nazemi Saeideh Sadat Shobeiri Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the Spinal Cord of Rats with Neuropathic Pain Journal of Mazandaran University of Medical Sciences neuropathic pain mesenchymal stem cell conditioned medium ccl3 ccl4 hyperalgesia allodynia |
| title | Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the
Spinal Cord of Rats with Neuropathic Pain |
| title_full | Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the
Spinal Cord of Rats with Neuropathic Pain |
| title_fullStr | Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the
Spinal Cord of Rats with Neuropathic Pain |
| title_full_unstemmed | Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the
Spinal Cord of Rats with Neuropathic Pain |
| title_short | Effect of Mesenchymal Stem Cells’ Conditioned Medium on the Expression of CCL3 and CCL4 Chemokines in the
Spinal Cord of Rats with Neuropathic Pain |
| title_sort | effect of mesenchymal stem cells conditioned medium on the expression of ccl3 and ccl4 chemokines in the spinal cord of rats with neuropathic pain |
| topic | neuropathic pain mesenchymal stem cell conditioned medium ccl3 ccl4 hyperalgesia allodynia |
| url | http://jmums.mazums.ac.ir/article-1-19756-en.pdf |
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