CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR
This study aimed to isolate and identify Aeromonas bacteria from samples of oysters and water, by microbiological and molecular methods. Identification of Aeromonas was performed by the conventional method and by PCR and species characterization was performed by identification key Aerokey II an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2014-09-01
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| Series: | Ciência Animal Brasileira |
| Subjects: | |
| Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/28351/17094 |
| _version_ | 1828484102242697216 |
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| author | Ana Carolina Miranda de Melo Silva Danielle Lopes do Nascimento Rosâgela Zacarias Machado Francisca Neide Costa |
| author_facet | Ana Carolina Miranda de Melo Silva Danielle Lopes do Nascimento Rosâgela Zacarias Machado Francisca Neide Costa |
| author_sort | Ana Carolina Miranda de Melo Silva |
| collection | DOAJ |
| description | This study aimed to isolate and identify Aeromonas
bacteria from samples of oysters and water, by
microbiological and molecular methods. Identification of
Aeromonas was performed by the conventional method
and by PCR and species characterization was performed
by identification key Aerokey II and by RFLP-PCR of
16S rDNA. 59 (98.3%) samples of oysters and 15 (75%)
samples of water contaminated by Aeromonas bacteria
were identified. Of the 74 isolates of Aeromonas spp.
obtained by microbiological analysis, 53 (71.62%), 38
(65.51%) oyster samples and 15 (100%) of water samples
confirmed the characterization of the genus by molecular
technique. As to biochemical species identification, 59.3%
(n = 35) and 40.6% (n = 24) of isolates from Aeromonas
sp. obtained from oyster samples were rated as A.
hydrophila and A. caviae, respectively; and from isolates
obtained from water samples, 60% (n = 9) were classified
as A. hydrophila and 40% (n = 6) as A. caviae; however, only the A. hydrophila was confirmed by genetic
sequencing. Regarding the classification by RFLP-PCR
16S rDNA, patterns of nonspecific bands were obtained,
making it impossible to identify the species by this
technique. The large number of oyester samples
contaminated with Aeromonas spp. and identification of
A. hydrophila, demonstrate that this mollusk might present
a risk to consumer health, mainly because it is consumed
raw and is to a potentially pathogenic micro-organism to
humans. |
| first_indexed | 2024-12-11T08:50:07Z |
| format | Article |
| id | doaj.art-50d0b9da1eff409c921ce59dc08f81bb |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-12-11T08:50:07Z |
| publishDate | 2014-09-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-50d0b9da1eff409c921ce59dc08f81bb2022-12-22T01:14:01ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912014-09-0115336236810.1590/1809-6891v15i328351CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULARAna Carolina Miranda de Melo Silva0Danielle Lopes do Nascimento1Rosâgela Zacarias Machado2Francisca Neide Costa3Pós Graduanda da Universidade Estadual do Maranhão, São Luiz, MA, Brasil.Médica Veterinária, Universidade Estadual do Maranhão, São Luiz, MA, Brasil.Professora Doutora da Universidade Estadual Paulista, Jaboticabal, SP, BrasilProfessora Doutora da Universidade Estadual do Maranhão, São Luiz, MA, BrasilThis study aimed to isolate and identify Aeromonas bacteria from samples of oysters and water, by microbiological and molecular methods. Identification of Aeromonas was performed by the conventional method and by PCR and species characterization was performed by identification key Aerokey II and by RFLP-PCR of 16S rDNA. 59 (98.3%) samples of oysters and 15 (75%) samples of water contaminated by Aeromonas bacteria were identified. Of the 74 isolates of Aeromonas spp. obtained by microbiological analysis, 53 (71.62%), 38 (65.51%) oyster samples and 15 (100%) of water samples confirmed the characterization of the genus by molecular technique. As to biochemical species identification, 59.3% (n = 35) and 40.6% (n = 24) of isolates from Aeromonas sp. obtained from oyster samples were rated as A. hydrophila and A. caviae, respectively; and from isolates obtained from water samples, 60% (n = 9) were classified as A. hydrophila and 40% (n = 6) as A. caviae; however, only the A. hydrophila was confirmed by genetic sequencing. Regarding the classification by RFLP-PCR 16S rDNA, patterns of nonspecific bands were obtained, making it impossible to identify the species by this technique. The large number of oyester samples contaminated with Aeromonas spp. and identification of A. hydrophila, demonstrate that this mollusk might present a risk to consumer health, mainly because it is consumed raw and is to a potentially pathogenic micro-organism to humans.http://www.revistas.ufg.br/index.php/vet/article/view/28351/17094AeromonasoystersPCRwater. |
| spellingShingle | Ana Carolina Miranda de Melo Silva Danielle Lopes do Nascimento Rosâgela Zacarias Machado Francisca Neide Costa CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR Ciência Animal Brasileira Aeromonas oysters PCR water. |
| title | CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR |
| title_full | CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR |
| title_fullStr | CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR |
| title_full_unstemmed | CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR |
| title_short | CARACTERIZAÇÃO DE AEROMONAS SPP ISOLADAS DE AMOSTRAS DE OSTRAS E ÁGUA POR MÉTODO MICROBIOLÓGICO E MOLECULAR |
| title_sort | caracterizacao de aeromonas spp isoladas de amostras de ostras e agua por metodo microbiologico e molecular |
| topic | Aeromonas oysters PCR water. |
| url | http://www.revistas.ufg.br/index.php/vet/article/view/28351/17094 |
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