Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
Label-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/11/467 |
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| author | Alessandro Esposito Alois Bonifacio Valter Sergo Stefano Fornasaro |
| author_facet | Alessandro Esposito Alois Bonifacio Valter Sergo Stefano Fornasaro |
| author_sort | Alessandro Esposito |
| collection | DOAJ |
| description | Label-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich samples such as serum or plasma can hinder the SERS effect due to protein–substrate interactions, they often require a deproteinization step. Moreover, SERS methods based on metal colloids often suffer from a poor reproducibility. Here, we propose a paper-based SERS sampling method in which unprocessed human serum samples are first soaked on paper strips (0.4 × 2 cm<sup>2</sup>), and then mixed with colloidal silver nanoparticles by centrifugation to obtain a Centrifugal Silver Plasmonic Paper (CSPP). The CSPP methodology has the potential to become a promising tool in bioanalytical SERS applications: it uses common colloidal substrates but without the need for sample deproteinization, while having a good reproducibility both in terms of overall spectral shape (r > 0.96) and absolute intensity (RSD < 10%). Moreover, this methodology allows SERS analysis more than one month after serum collection on the paper strip, facilitating storage and handling of clinical samples (including shipping from clinical sites to labs). |
| first_indexed | 2024-03-10T05:40:12Z |
| format | Article |
| id | doaj.art-50d1b2237e58452a80c403c4b8ada0b3 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T05:40:12Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-50d1b2237e58452a80c403c4b8ada0b32023-11-22T22:36:36ZengMDPI AGBiosensors2079-63742021-11-01111146710.3390/bios11110467Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and AnalysisAlessandro Esposito0Alois Bonifacio1Valter Sergo2Stefano Fornasaro3Raman Spectroscopy Lab, Department of Engineering and Architecture, University of Trieste, 34100 Trieste, ItalyRaman Spectroscopy Lab, Department of Engineering and Architecture, University of Trieste, 34100 Trieste, ItalyRaman Spectroscopy Lab, Department of Engineering and Architecture, University of Trieste, 34100 Trieste, ItalyRaman Spectroscopy Lab, Department of Engineering and Architecture, University of Trieste, 34100 Trieste, ItalyLabel-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich samples such as serum or plasma can hinder the SERS effect due to protein–substrate interactions, they often require a deproteinization step. Moreover, SERS methods based on metal colloids often suffer from a poor reproducibility. Here, we propose a paper-based SERS sampling method in which unprocessed human serum samples are first soaked on paper strips (0.4 × 2 cm<sup>2</sup>), and then mixed with colloidal silver nanoparticles by centrifugation to obtain a Centrifugal Silver Plasmonic Paper (CSPP). The CSPP methodology has the potential to become a promising tool in bioanalytical SERS applications: it uses common colloidal substrates but without the need for sample deproteinization, while having a good reproducibility both in terms of overall spectral shape (r > 0.96) and absolute intensity (RSD < 10%). Moreover, this methodology allows SERS analysis more than one month after serum collection on the paper strip, facilitating storage and handling of clinical samples (including shipping from clinical sites to labs).https://www.mdpi.com/2079-6374/11/11/467label-free SERSbiofluidsplasmonic papervibrational spectroscopyserum |
| spellingShingle | Alessandro Esposito Alois Bonifacio Valter Sergo Stefano Fornasaro Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis Biosensors label-free SERS biofluids plasmonic paper vibrational spectroscopy serum |
| title | Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis |
| title_full | Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis |
| title_fullStr | Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis |
| title_full_unstemmed | Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis |
| title_short | Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis |
| title_sort | label free surface enhanced raman scattering sers on centrifugal silver plasmonic paper cspp a novel methodology for unprocessed biofluids sampling and analysis |
| topic | label-free SERS biofluids plasmonic paper vibrational spectroscopy serum |
| url | https://www.mdpi.com/2079-6374/11/11/467 |
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