Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control
Graves' ophthalmopathy (GO) is an extrathyroidal manifestation of Graves' disease, Orbital fibroblasts (OFs) are recognized as key players in GO pathogenesis, involved in orbital inflammation, tissue remodeling, and fibrosis. This study offers a primary exploration of cell behavior and cha...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024044281 |
| _version_ | 1827307832061460480 |
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| author | Yu Wu Jiuming Zhang Wen Deng Chaoting Mo Yumei Liang Kongqian Huang Fan Xu Fen Tang |
| author_facet | Yu Wu Jiuming Zhang Wen Deng Chaoting Mo Yumei Liang Kongqian Huang Fan Xu Fen Tang |
| author_sort | Yu Wu |
| collection | DOAJ |
| description | Graves' ophthalmopathy (GO) is an extrathyroidal manifestation of Graves' disease, Orbital fibroblasts (OFs) are recognized as key players in GO pathogenesis, involved in orbital inflammation, tissue remodeling, and fibrosis. This study offers a primary exploration of cell behavior and characteristics on OFs from GO (GO-OFs), and compared to OFs from healthy control (HC-OFs). Results reveal that GO-OFs exhibit delayed migration from tissue fragments, while no significant difference in cell proliferation is observed between GO-OFs and HC-OFs. Aberrant expression pattern of surface proteins Thy-1, TSHR, and IGF-1R suggests shared autoantigens and pathways between GO and GD, contributing to inflammation and fibrosis. Investigations into cytokine responses unveil elevated secretion of hyaluronic acid (HA) and prostaglandin E2 (PGE2) in GO-OFs, emphasizing their role in tissue remodeling. These findings deepen our understanding of OFs in GO pathogenesis, offering potential therapeutic avenues. |
| first_indexed | 2024-04-24T18:47:19Z |
| format | Article |
| id | doaj.art-50d35fbaa0824c2f80304164e0479543 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T18:47:19Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-50d35fbaa0824c2f80304164e04795432024-03-27T04:52:36ZengElsevierHeliyon2405-84402024-04-01107e28397Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy controlYu Wu0Jiuming Zhang1Wen Deng2Chaoting Mo3Yumei Liang4Kongqian Huang5Fan Xu6Fen Tang7Department of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, ChinaDepartment of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, ChinaDepartment of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, ChinaDepartment of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, China; Youjiang Medical University for Nationalities, Baise, 531400, ChinaDepartment of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, China; Guilin Medical University, Guilin, 541000, ChinaDepartment of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, ChinaDepartment of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, China; Corresponding author.Department of Ophthalmology, the People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Key Laboratory of Eye Health & Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology &Institute of Ophthalmic Diseases, Guangxi Academy of Medical Sciences, Nanning, 530021, China; Corresponding author.Graves' ophthalmopathy (GO) is an extrathyroidal manifestation of Graves' disease, Orbital fibroblasts (OFs) are recognized as key players in GO pathogenesis, involved in orbital inflammation, tissue remodeling, and fibrosis. This study offers a primary exploration of cell behavior and characteristics on OFs from GO (GO-OFs), and compared to OFs from healthy control (HC-OFs). Results reveal that GO-OFs exhibit delayed migration from tissue fragments, while no significant difference in cell proliferation is observed between GO-OFs and HC-OFs. Aberrant expression pattern of surface proteins Thy-1, TSHR, and IGF-1R suggests shared autoantigens and pathways between GO and GD, contributing to inflammation and fibrosis. Investigations into cytokine responses unveil elevated secretion of hyaluronic acid (HA) and prostaglandin E2 (PGE2) in GO-OFs, emphasizing their role in tissue remodeling. These findings deepen our understanding of OFs in GO pathogenesis, offering potential therapeutic avenues.http://www.sciencedirect.com/science/article/pii/S2405844024044281Graves' ophthalmopathyOrbital fibroblastsTSHRIGF-1RHyaluronic acidProstaglandin E2 |
| spellingShingle | Yu Wu Jiuming Zhang Wen Deng Chaoting Mo Yumei Liang Kongqian Huang Fan Xu Fen Tang Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control Heliyon Graves' ophthalmopathy Orbital fibroblasts TSHR IGF-1R Hyaluronic acid Prostaglandin E2 |
| title | Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control |
| title_full | Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control |
| title_fullStr | Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control |
| title_full_unstemmed | Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control |
| title_short | Comparison of orbital fibroblasts from Graves’ ophthalmopathy and healthy control |
| title_sort | comparison of orbital fibroblasts from graves ophthalmopathy and healthy control |
| topic | Graves' ophthalmopathy Orbital fibroblasts TSHR IGF-1R Hyaluronic acid Prostaglandin E2 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024044281 |
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