Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis
Abstract Background GeneXpert enterovirus Assay is a PCR-based assay for Enterovirus meningitis diagnosis. However, there is currently no research about the performance of GeneXpert enterovirus assay in the diagnosis of enterovirus meningitis. Thus, a systematic review and meta-analysis is significa...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-022-00517-3 |
| _version_ | 1828554487385554944 |
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| author | Min Lin Yun-Ran Li Qi-Wen Lan Li-Jun Long Jia-Qi Liu Ying-Wen Chen Xun-Jie Cao Ge-Yuan Wu Ya-Ping Li Xu-Guang Guo |
| author_facet | Min Lin Yun-Ran Li Qi-Wen Lan Li-Jun Long Jia-Qi Liu Ying-Wen Chen Xun-Jie Cao Ge-Yuan Wu Ya-Ping Li Xu-Guang Guo |
| author_sort | Min Lin |
| collection | DOAJ |
| description | Abstract Background GeneXpert enterovirus Assay is a PCR-based assay for Enterovirus meningitis diagnosis. However, there is currently no research about the performance of GeneXpert enterovirus assay in the diagnosis of enterovirus meningitis. Thus, a systematic review and meta-analysis is significant on the topic. Methods Embase, Cochrane Library, Web of Science, and PubMed were systematically reviewed with retrieval types. Some criteria were used to filter the studies. Only studies published in English, that made a comparison between GeneXpert enterovirus assay and RT-PCR, and could be formulated in a 2*2 table, were included. The quality of the included studies was evaluated by QUADAS-2. The effect of the GeneXpert enterovirus assay was assessed by the Sensitivity, Specificity, Positive Likelihood Ratio, Negative Likelihood Ratio, Diagnosis Odds Ratio, and summary receiver operating characteristic (SROC) curve. Publication bias and heterogeneity were evaluated by the Deeks' funnel test and Bivariate Box plot respectively. Results 7 studies were recruited in the analysis. The Pooled Sensitivity was 0.96 [95% CI (0.94–0.97)], Pooled Specificity was 0.99 [95% CI (0.98–0.99)], Positive Likelihood Ratio was 130.46 [95% CI (35.79–475.58)], Negative Likelihood Ratio was 0.04 [95% CI (0.02–0.10)], and Diagnostic Odds Ratio was 3648.23 (95% CI [963.99–13,806.72)]. In SROC Curve, Area Under Curve (AUC) was 0.9980, and Q*= 0.9849. In Deeks' funnel test, the P-value was 0.807 (P > 0.05), indicating no publication bias. The Bivariate Box plot indicated no evident heterogeneity. Conclusions The GeneXpert enterovirus assay demonstrated high diagnostic accuracy in diagnosing enterovirus meningitis. |
| first_indexed | 2024-12-12T05:36:38Z |
| format | Article |
| id | doaj.art-50d45e9ad9674fd1ab62d10e0800f480 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-12T05:36:38Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-50d45e9ad9674fd1ab62d10e0800f4802022-12-22T00:36:06ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112022-06-012111810.1186/s12941-022-00517-3Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysisMin Lin0Yun-Ran Li1Qi-Wen Lan2Li-Jun Long3Jia-Qi Liu4Ying-Wen Chen5Xun-Jie Cao6Ge-Yuan Wu7Ya-Ping Li8Xu-Guang Guo9Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Chinese and Western Medicine in Clinical Medicine, The Clinical School of Chinese and Western Medicine, Guangzhou Medical UniversityDepartment of Medical Imageology, The Second Clinical School of Guangzhou Medical UniversityDepartment of Medical Laboratory Technology, The KingMed School of Laboratory Medicine, Guangzhou Medical UniversityDepartment of Chinese and Western Medicine in Clinical Medicine, The Clinical School of Chinese and Western Medicine, Guangzhou Medical UniversityDepartment of Chinese and Western Medicine in Clinical Medicine, The Clinical School of Chinese and Western Medicine, Guangzhou Medical UniversityDepartment of Clinical Medicine, The Third Clinical School of Guangzhou Medical UniversityDepartment of Chinese and Western Medicine in Clinical Medicine, The Clinical School of Chinese and Western Medicine, Guangzhou Medical UniversityDepartment of Clinical Medicine, The Second Clinical School of Guangzhou Medical UniversityDepartment of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityAbstract Background GeneXpert enterovirus Assay is a PCR-based assay for Enterovirus meningitis diagnosis. However, there is currently no research about the performance of GeneXpert enterovirus assay in the diagnosis of enterovirus meningitis. Thus, a systematic review and meta-analysis is significant on the topic. Methods Embase, Cochrane Library, Web of Science, and PubMed were systematically reviewed with retrieval types. Some criteria were used to filter the studies. Only studies published in English, that made a comparison between GeneXpert enterovirus assay and RT-PCR, and could be formulated in a 2*2 table, were included. The quality of the included studies was evaluated by QUADAS-2. The effect of the GeneXpert enterovirus assay was assessed by the Sensitivity, Specificity, Positive Likelihood Ratio, Negative Likelihood Ratio, Diagnosis Odds Ratio, and summary receiver operating characteristic (SROC) curve. Publication bias and heterogeneity were evaluated by the Deeks' funnel test and Bivariate Box plot respectively. Results 7 studies were recruited in the analysis. The Pooled Sensitivity was 0.96 [95% CI (0.94–0.97)], Pooled Specificity was 0.99 [95% CI (0.98–0.99)], Positive Likelihood Ratio was 130.46 [95% CI (35.79–475.58)], Negative Likelihood Ratio was 0.04 [95% CI (0.02–0.10)], and Diagnostic Odds Ratio was 3648.23 (95% CI [963.99–13,806.72)]. In SROC Curve, Area Under Curve (AUC) was 0.9980, and Q*= 0.9849. In Deeks' funnel test, the P-value was 0.807 (P > 0.05), indicating no publication bias. The Bivariate Box plot indicated no evident heterogeneity. Conclusions The GeneXpert enterovirus assay demonstrated high diagnostic accuracy in diagnosing enterovirus meningitis.https://doi.org/10.1186/s12941-022-00517-3GeneXpert EVEnterovirus meningitisPCRSystematic-reviewMeta-analysis |
| spellingShingle | Min Lin Yun-Ran Li Qi-Wen Lan Li-Jun Long Jia-Qi Liu Ying-Wen Chen Xun-Jie Cao Ge-Yuan Wu Ya-Ping Li Xu-Guang Guo Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis Annals of Clinical Microbiology and Antimicrobials GeneXpert EV Enterovirus meningitis PCR Systematic-review Meta-analysis |
| title | Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis |
| title_full | Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis |
| title_fullStr | Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis |
| title_full_unstemmed | Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis |
| title_short | Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis |
| title_sort | evaluation of genexpert ev assay for the rapid diagnosis of enteroviral meningitis a systematic review and meta analysis |
| topic | GeneXpert EV Enterovirus meningitis PCR Systematic-review Meta-analysis |
| url | https://doi.org/10.1186/s12941-022-00517-3 |
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