Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes
Endurance exercise induces skeletal muscle adaptations such as fiber-type switching, mitochondrial biogenesis, angiogenesis, and the enhancement of glucose disposal, all of which ameliorate metabolic dysfunction. Since many factors such as body temperature, pH, osmolality, the secretion patterns of...
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| Format: | Article |
| Language: | English |
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Japanese Society of Physical Fitness and Sports Medicine
2022-01-01
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| Series: | Journal of Physical Fitness and Sports Medicine |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpfsm/11/1/11_51/_pdf/-char/en |
| _version_ | 1818965998921842688 |
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| author | Yoshitaka Mita Miyuki Ito Mio Yamada Nobuharu L. Fujii Yasuko Manabe Yasuro Furuichi |
| author_facet | Yoshitaka Mita Miyuki Ito Mio Yamada Nobuharu L. Fujii Yasuko Manabe Yasuro Furuichi |
| author_sort | Yoshitaka Mita |
| collection | DOAJ |
| description | Endurance exercise induces skeletal muscle adaptations such as fiber-type switching, mitochondrial biogenesis, angiogenesis, and the enhancement of glucose disposal, all of which ameliorate metabolic dysfunction. Since many factors such as body temperature, pH, osmolality, the secretion patterns of neurotransmitters, and humoral factors, change during exercise, it is not easy to determine precisely how each factor contributes to exercise-induced adaptations. To determine these contributions, there is need for experimental studies using an in vitro muscle culture system focusing on a single added stimulus. In this study, we focused on whether contractile stimulation is itself responsible for inducing skeletal muscle adaptations. We constructed a chronic contraction model in mouse primary myotubes and investigated which type of contractile stimulation could induce muscle fiber switching and/or metabolic adaptations. We tested five sets of contractile stimulus conditions, including tetanus and twitch, for different stimulation periods. Of these, when myotubes were stimulated by 6 V/15 mA electric pulses at 1 Hz (20 ms contraction followed by 980 ms relaxation) for 24 hours, we observed a significant increase in the expression of myosin heavy chain (MyHC) I protein, a marker protein for type I (oxidative) myofiber, and a tendency for MyHC IIa expression to increase, a marker protein for type IIa fiber (the most oxidative myofiber out of the type II isoforms). However, the same conditions did not induce any change in the expression of GLUT4, COX IV, and hexokinase II, proteins related to the transport of glucose and metabolism. These results suggest chronic contractile stimulation does not induce the expression of proteins related to metabolism, but it does regulate the expression patterns of MyHC. This chronic contraction model has the potential to clarify the molecular mechanisms underlying the induction of oxidative myofibers in response to muscle contraction. |
| first_indexed | 2024-12-20T13:25:55Z |
| format | Article |
| id | doaj.art-50d50c04966643df993a0e368948ec28 |
| institution | Directory Open Access Journal |
| issn | 2186-8131 2186-8123 |
| language | English |
| last_indexed | 2024-12-20T13:25:55Z |
| publishDate | 2022-01-01 |
| publisher | Japanese Society of Physical Fitness and Sports Medicine |
| record_format | Article |
| series | Journal of Physical Fitness and Sports Medicine |
| spelling | doaj.art-50d50c04966643df993a0e368948ec282022-12-21T19:39:16ZengJapanese Society of Physical Fitness and Sports MedicineJournal of Physical Fitness and Sports Medicine2186-81312186-81232022-01-01111515610.7600/jpfsm.11.51jpfsmEffect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubesYoshitaka Mita0Miyuki Ito1Mio Yamada2Nobuharu L. Fujii3Yasuko Manabe4Yasuro Furuichi5Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan UniversityDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan UniversityDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan UniversityDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan UniversityDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan UniversityDepartment of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan UniversityEndurance exercise induces skeletal muscle adaptations such as fiber-type switching, mitochondrial biogenesis, angiogenesis, and the enhancement of glucose disposal, all of which ameliorate metabolic dysfunction. Since many factors such as body temperature, pH, osmolality, the secretion patterns of neurotransmitters, and humoral factors, change during exercise, it is not easy to determine precisely how each factor contributes to exercise-induced adaptations. To determine these contributions, there is need for experimental studies using an in vitro muscle culture system focusing on a single added stimulus. In this study, we focused on whether contractile stimulation is itself responsible for inducing skeletal muscle adaptations. We constructed a chronic contraction model in mouse primary myotubes and investigated which type of contractile stimulation could induce muscle fiber switching and/or metabolic adaptations. We tested five sets of contractile stimulus conditions, including tetanus and twitch, for different stimulation periods. Of these, when myotubes were stimulated by 6 V/15 mA electric pulses at 1 Hz (20 ms contraction followed by 980 ms relaxation) for 24 hours, we observed a significant increase in the expression of myosin heavy chain (MyHC) I protein, a marker protein for type I (oxidative) myofiber, and a tendency for MyHC IIa expression to increase, a marker protein for type IIa fiber (the most oxidative myofiber out of the type II isoforms). However, the same conditions did not induce any change in the expression of GLUT4, COX IV, and hexokinase II, proteins related to the transport of glucose and metabolism. These results suggest chronic contractile stimulation does not induce the expression of proteins related to metabolism, but it does regulate the expression patterns of MyHC. This chronic contraction model has the potential to clarify the molecular mechanisms underlying the induction of oxidative myofibers in response to muscle contraction.https://www.jstage.jst.go.jp/article/jpfsm/11/1/11_51/_pdf/-char/enskeletal musclecontractile myotubeelectric pulse stimulationprimary cultured cell |
| spellingShingle | Yoshitaka Mita Miyuki Ito Mio Yamada Nobuharu L. Fujii Yasuko Manabe Yasuro Furuichi Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes Journal of Physical Fitness and Sports Medicine skeletal muscle contractile myotube electric pulse stimulation primary cultured cell |
| title | Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes |
| title_full | Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes |
| title_fullStr | Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes |
| title_full_unstemmed | Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes |
| title_short | Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes |
| title_sort | effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes |
| topic | skeletal muscle contractile myotube electric pulse stimulation primary cultured cell |
| url | https://www.jstage.jst.go.jp/article/jpfsm/11/1/11_51/_pdf/-char/en |
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