Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation
abstract: With the aim of contributing to the development of assisted reproduction for red-rumped agouti germplasm conservation, we compared different anesthetic protocols for electroejaculation in agoutis in terms of anesthetic parameters, functions of erection and ejaculation, and semen metrics. T...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Theriogenology Wild |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773093X23000041 |
| _version_ | 1797801632843431936 |
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| author | Amara Gyane Alves de Lima Maria Gláucia Carlos de Oliveira Isabelle de Oliveira Lima Kathryn Nóbrega Arcoverde Lívia Batista Campos Andreia Maria da Silva Thibério de Souza Castelo Moacir Franco de Oliveira Alexandre Rodrigues Silva Valéria Veras de Paula |
| author_facet | Amara Gyane Alves de Lima Maria Gláucia Carlos de Oliveira Isabelle de Oliveira Lima Kathryn Nóbrega Arcoverde Lívia Batista Campos Andreia Maria da Silva Thibério de Souza Castelo Moacir Franco de Oliveira Alexandre Rodrigues Silva Valéria Veras de Paula |
| author_sort | Amara Gyane Alves de Lima |
| collection | DOAJ |
| description | abstract: With the aim of contributing to the development of assisted reproduction for red-rumped agouti germplasm conservation, we compared different anesthetic protocols for electroejaculation in agoutis in terms of anesthetic parameters, functions of erection and ejaculation, and semen metrics. Ten sexually mature males underwent electroejaculation with 15-day intervals according to the following anesthetic protocols: Ketamine/xylazine, Ketamine/dexmedetomidine, Ketamine/xylazine/epidural anesthesia or Azaperone/meperidine/ketamine/xylazine plus epidural anesthesia. The following variables were evaluated: induction latency period, anesthetic recovery, and duration of epidural anesthesia. Regarding the reproductive variables, erection, the stimulation cycle in which ejaculation occurred, and the semen characteristics were observed. All the protocols were efficient for providing adequate induction of latency (ranging from 1.46 to 13.14 min) and recovery (average 2 h) periods. The use of a premedication did not influence the duration of epidural anesthesia (P<0.05). The administration of an azaperone/meperidine-based premedication prior to epidural anesthesia promoted an increase in the agouti erection process when compared to the other protocols (P < 0.05). All the protocols were efficient in stimulating ejaculation (100 %), even though most ejaculates were aspermic (85 %). The presence of sperm was only observed in 30 % of the animals anesthetized with ketamine/xylazine, 20 % with ketamine/dexmedetomidine, and 10 % with Ketamine/xylazine/epidural anesthesia. Ketamine/xylazine provided a lower volume (201.7±173.4 vs. 810.0±127.3 µL) and greater sperm concentration (270.0±200 vs. 25.0±15.0 × 106 sperm/mL) than Ketamine/dexmedetomidine (P < 0.05). In conclusion, we suggest the use of the standard anesthetic protocol based on the Ketamine/xylazine combination to obtain sperm in red-rumped agouti. Data availability statement: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. |
| first_indexed | 2024-03-13T04:53:25Z |
| format | Article |
| id | doaj.art-50d73013b48246debbe5ac4aa31d4c2e |
| institution | Directory Open Access Journal |
| issn | 2773-093X |
| language | English |
| last_indexed | 2024-03-13T04:53:25Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Theriogenology Wild |
| spelling | doaj.art-50d73013b48246debbe5ac4aa31d4c2e2023-06-18T05:04:25ZengElsevierTheriogenology Wild2773-093X2023-01-012100020Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculationAmara Gyane Alves de Lima0Maria Gláucia Carlos de Oliveira1Isabelle de Oliveira Lima2Kathryn Nóbrega Arcoverde3Lívia Batista Campos4Andreia Maria da Silva5Thibério de Souza Castelo6Moacir Franco de Oliveira7Alexandre Rodrigues Silva8Valéria Veras de Paula9Department of Animal Science, Universidade Federal Rural do Semi-Árido, 59625–900 Mossoró, RN, BrazilDepartment of Animal Science, Universidade Federal Rural do Semi-Árido, 59625–900 Mossoró, RN, BrazilDepartment of Animal Science, Universidade Federal Rural do Semi-Árido, 59625–900 Mossoró, RN, BrazilDepartment of Animal Science, Universidade Federal Rural do Semi-Árido, 59625–900 Mossoró, RN, BrazilLaboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, 59625–900 Mossoró, RN, BrazilLaboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, 59625–900 Mossoró, RN, BrazilLaboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, 59625–900 Mossoró, RN, BrazilDepartment of Animal Science, Universidade Federal Rural do Semi-Árido, 59625–900 Mossoró, RN, BrazilLaboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, 59625–900 Mossoró, RN, BrazilDepartment of Animal Science, Universidade Federal Rural do Semi-Árido, 59625–900 Mossoró, RN, Brazil; Correspondence to: Departamento de Ciências Animais, Universidade Federal Rural do Semi-árido, Mossoró, Rio Grande do Norte, Brazil.abstract: With the aim of contributing to the development of assisted reproduction for red-rumped agouti germplasm conservation, we compared different anesthetic protocols for electroejaculation in agoutis in terms of anesthetic parameters, functions of erection and ejaculation, and semen metrics. Ten sexually mature males underwent electroejaculation with 15-day intervals according to the following anesthetic protocols: Ketamine/xylazine, Ketamine/dexmedetomidine, Ketamine/xylazine/epidural anesthesia or Azaperone/meperidine/ketamine/xylazine plus epidural anesthesia. The following variables were evaluated: induction latency period, anesthetic recovery, and duration of epidural anesthesia. Regarding the reproductive variables, erection, the stimulation cycle in which ejaculation occurred, and the semen characteristics were observed. All the protocols were efficient for providing adequate induction of latency (ranging from 1.46 to 13.14 min) and recovery (average 2 h) periods. The use of a premedication did not influence the duration of epidural anesthesia (P<0.05). The administration of an azaperone/meperidine-based premedication prior to epidural anesthesia promoted an increase in the agouti erection process when compared to the other protocols (P < 0.05). All the protocols were efficient in stimulating ejaculation (100 %), even though most ejaculates were aspermic (85 %). The presence of sperm was only observed in 30 % of the animals anesthetized with ketamine/xylazine, 20 % with ketamine/dexmedetomidine, and 10 % with Ketamine/xylazine/epidural anesthesia. Ketamine/xylazine provided a lower volume (201.7±173.4 vs. 810.0±127.3 µL) and greater sperm concentration (270.0±200 vs. 25.0±15.0 × 106 sperm/mL) than Ketamine/dexmedetomidine (P < 0.05). In conclusion, we suggest the use of the standard anesthetic protocol based on the Ketamine/xylazine combination to obtain sperm in red-rumped agouti. Data availability statement: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.http://www.sciencedirect.com/science/article/pii/S2773093X23000041WildlifeRodentsAlpha2-agoniztsKetamineEpidural anesthesiaSemen |
| spellingShingle | Amara Gyane Alves de Lima Maria Gláucia Carlos de Oliveira Isabelle de Oliveira Lima Kathryn Nóbrega Arcoverde Lívia Batista Campos Andreia Maria da Silva Thibério de Souza Castelo Moacir Franco de Oliveira Alexandre Rodrigues Silva Valéria Veras de Paula Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation Theriogenology Wild Wildlife Rodents Alpha2-agonizts Ketamine Epidural anesthesia Semen |
| title | Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation |
| title_full | Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation |
| title_fullStr | Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation |
| title_full_unstemmed | Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation |
| title_short | Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation |
| title_sort | effect of different anesthetic protocols on erection ejaculation and sperm parameters in red rumped agoutis dasyprocta leporina subjected to electroejaculation |
| topic | Wildlife Rodents Alpha2-agonizts Ketamine Epidural anesthesia Semen |
| url | http://www.sciencedirect.com/science/article/pii/S2773093X23000041 |
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