Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing
Salt dietary intake is tightly coupled to human health, and excessive sodium can cause strokes and cardiovascular diseases. Research into the renal medulla of camels exhibiting high salt resistance may aid identification of the mechanisms governing resistance to high salinity. In this study, we used...
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| Format: | Article |
| Language: | English |
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Wiley
2022-04-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13380 |
| _version_ | 1828873888846577664 |
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| author | Dong Zhang Jing Pan Chunxia Liu Fanhua Meng Yanru Zhang Junwei Cao Yu Cao Huanmin Zhou |
| author_facet | Dong Zhang Jing Pan Chunxia Liu Fanhua Meng Yanru Zhang Junwei Cao Yu Cao Huanmin Zhou |
| author_sort | Dong Zhang |
| collection | DOAJ |
| description | Salt dietary intake is tightly coupled to human health, and excessive sodium can cause strokes and cardiovascular diseases. Research into the renal medulla of camels exhibiting high salt resistance may aid identification of the mechanisms governing resistance to high salinity. In this study, we used RNA sequencing (RNA‐seq) to show that in the renal medulla of camels under salt stress, 22 mRNAs, 2 long noncoding RNAs (lncRNAs), and 31 microRNAs (miRNAs) exhibited differential expression compared with the free salt‐intake diet group. Using fluorescence in situ hybridization and dual‐luciferase reporter assays, we demonstrated that the lncRNA LNC003834 can bind miRNA‐34a and thereby relieve suppression of the salt‐absorption‐inhibiting SLC14A1 mRNA from miRNA‐34a, suggesting that the above lncRNA‐miRNA‐mRNA act as competing endogenous RNAs (ceRNAs). We subsequently performed short hairpin RNA and small RNA interference and reactive oxygen species (ROS) detection assays to show that SLC6A1, PCBP2, and PEX5L can improve the antioxidation capacity of renal medulla cells of camel by decreasing ROS levels. Our data suggest that camels achieve sodium homeostasis through regulating the expression of salt‐reabsorption‐related genes in the renal medulla, and this involves ceRNAs (SLC14A1 mRNA, LNC003834, and miRNA‐34a) and antioxidant genes (SLC6A1, PCBP2, and PEX5L). These data may assist in the development of treatments for diseases induced by high salt diets. |
| first_indexed | 2024-12-13T07:24:30Z |
| format | Article |
| id | doaj.art-50d7a46b5ea14c0b86efa9ebb29b4f17 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-13T07:24:30Z |
| publishDate | 2022-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-50d7a46b5ea14c0b86efa9ebb29b4f172022-12-21T23:55:19ZengWileyFEBS Open Bio2211-54632022-04-0112486487610.1002/2211-5463.13380Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencingDong Zhang0Jing Pan1Chunxia Liu2Fanhua Meng3Yanru Zhang4Junwei Cao5Yu Cao6Huanmin Zhou7Inner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaInner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaInner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaInner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaInner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaInner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaInner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaInner Mongolia Key Laboratory of Bio‐manufacture Inner Mongolia Agricultural University Hohhot ChinaSalt dietary intake is tightly coupled to human health, and excessive sodium can cause strokes and cardiovascular diseases. Research into the renal medulla of camels exhibiting high salt resistance may aid identification of the mechanisms governing resistance to high salinity. In this study, we used RNA sequencing (RNA‐seq) to show that in the renal medulla of camels under salt stress, 22 mRNAs, 2 long noncoding RNAs (lncRNAs), and 31 microRNAs (miRNAs) exhibited differential expression compared with the free salt‐intake diet group. Using fluorescence in situ hybridization and dual‐luciferase reporter assays, we demonstrated that the lncRNA LNC003834 can bind miRNA‐34a and thereby relieve suppression of the salt‐absorption‐inhibiting SLC14A1 mRNA from miRNA‐34a, suggesting that the above lncRNA‐miRNA‐mRNA act as competing endogenous RNAs (ceRNAs). We subsequently performed short hairpin RNA and small RNA interference and reactive oxygen species (ROS) detection assays to show that SLC6A1, PCBP2, and PEX5L can improve the antioxidation capacity of renal medulla cells of camel by decreasing ROS levels. Our data suggest that camels achieve sodium homeostasis through regulating the expression of salt‐reabsorption‐related genes in the renal medulla, and this involves ceRNAs (SLC14A1 mRNA, LNC003834, and miRNA‐34a) and antioxidant genes (SLC6A1, PCBP2, and PEX5L). These data may assist in the development of treatments for diseases induced by high salt diets.https://doi.org/10.1002/2211-5463.13380camelceRNAsrenal medullasalt resistancesodium homeostasis |
| spellingShingle | Dong Zhang Jing Pan Chunxia Liu Fanhua Meng Yanru Zhang Junwei Cao Yu Cao Huanmin Zhou Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing FEBS Open Bio camel ceRNAs renal medulla salt resistance sodium homeostasis |
| title | Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing |
| title_full | Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing |
| title_fullStr | Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing |
| title_full_unstemmed | Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing |
| title_short | Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing |
| title_sort | identification of sodium homeostasis genes in camelus bactrianus by whole transcriptome sequencing |
| topic | camel ceRNAs renal medulla salt resistance sodium homeostasis |
| url | https://doi.org/10.1002/2211-5463.13380 |
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