Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis
Sporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa are the main causative agents of sporotrichosis, a human subcutaneous mycosis. Differences in virulence patterns are associated with each species but remain largely uncharacterized. The S. schenckii and S. brasiliensis cell wall c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.726958/full |
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| author | Héctor L. Villalobos-Duno Laura A. Barreto Álvaro Alvarez-Aular Héctor M. Mora-Montes Nancy E. Lozoya-Pérez Bernardo Franco Leila M. Lopes-Bezerra Gustavo A. Niño-Vega |
| author_facet | Héctor L. Villalobos-Duno Laura A. Barreto Álvaro Alvarez-Aular Héctor M. Mora-Montes Nancy E. Lozoya-Pérez Bernardo Franco Leila M. Lopes-Bezerra Gustavo A. Niño-Vega |
| author_sort | Héctor L. Villalobos-Duno |
| collection | DOAJ |
| description | Sporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa are the main causative agents of sporotrichosis, a human subcutaneous mycosis. Differences in virulence patterns are associated with each species but remain largely uncharacterized. The S. schenckii and S. brasiliensis cell wall composition and virulence are influenced by the culturing media, with little or no influence on S. globosa. By keeping constant the culturing media, we compared the cell wall composition of three S. schenckii and two S. brasiliensis strains, previously described as presenting different virulence levels on a murine model of infection. The cell wall composition of the five Sporothrix spp. strains correlated with the biochemical composition of the cell wall previously reported for the species. However, the rhamnose-to-β-glucan ratio exhibits differences among strains, with an increase in cell wall rhamnose-to-β-glucan ratio as their virulence increased. This relationship can be expressed mathematically, which could be an important tool for the determination of virulence in Sporothrix spp. Also, structural differences in rhamnomannan were found, with longer side chains present in strains with lower virulence reported for both species here studied, adding insight to the importance of this polysaccharide in the pathogenic process of these fungi. |
| first_indexed | 2024-12-21T15:20:49Z |
| format | Article |
| id | doaj.art-50daab34ac324dfd827103f53cdfcb8e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T15:20:49Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-50daab34ac324dfd827103f53cdfcb8e2022-12-21T18:59:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-09-011210.3389/fmicb.2021.726958726958Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensisHéctor L. Villalobos-Duno0Laura A. Barreto1Álvaro Alvarez-Aular2Héctor M. Mora-Montes3Nancy E. Lozoya-Pérez4Bernardo Franco5Leila M. Lopes-Bezerra6Gustavo A. Niño-Vega7Laboratorio de Micología, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, VenezuelaInstituto Superior de Formación Docente Salome Ureña, Santo Domingo, Dominican RepublicLaboratorio de Síntesis Orgánica y Productos Naturales, Centro de Química, Instituto Venezolano de Investigaciones Científicas, Caracas, VenezuelaDivisión de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Guanajuato, MexicoDivisión de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Guanajuato, MexicoDivisión de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Guanajuato, MexicoBiomedical Institute, University of São Paulo, São Paulo, BrazilDivisión de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Guanajuato, MexicoSporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa are the main causative agents of sporotrichosis, a human subcutaneous mycosis. Differences in virulence patterns are associated with each species but remain largely uncharacterized. The S. schenckii and S. brasiliensis cell wall composition and virulence are influenced by the culturing media, with little or no influence on S. globosa. By keeping constant the culturing media, we compared the cell wall composition of three S. schenckii and two S. brasiliensis strains, previously described as presenting different virulence levels on a murine model of infection. The cell wall composition of the five Sporothrix spp. strains correlated with the biochemical composition of the cell wall previously reported for the species. However, the rhamnose-to-β-glucan ratio exhibits differences among strains, with an increase in cell wall rhamnose-to-β-glucan ratio as their virulence increased. This relationship can be expressed mathematically, which could be an important tool for the determination of virulence in Sporothrix spp. Also, structural differences in rhamnomannan were found, with longer side chains present in strains with lower virulence reported for both species here studied, adding insight to the importance of this polysaccharide in the pathogenic process of these fungi.https://www.frontiersin.org/articles/10.3389/fmicb.2021.726958/fullSporothrix spp.fungal cell wallbeta-gucanfungal virulenceRhamnoseRhamnomannan |
| spellingShingle | Héctor L. Villalobos-Duno Laura A. Barreto Álvaro Alvarez-Aular Héctor M. Mora-Montes Nancy E. Lozoya-Pérez Bernardo Franco Leila M. Lopes-Bezerra Gustavo A. Niño-Vega Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis Frontiers in Microbiology Sporothrix spp. fungal cell wall beta-gucan fungal virulence Rhamnose Rhamnomannan |
| title | Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis |
| title_full | Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis |
| title_fullStr | Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis |
| title_full_unstemmed | Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis |
| title_short | Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis |
| title_sort | comparison of cell wall polysaccharide composition and structure between strains of sporothrix schenckii and sporothrix brasiliensis |
| topic | Sporothrix spp. fungal cell wall beta-gucan fungal virulence Rhamnose Rhamnomannan |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.726958/full |
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