Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i>
Green synthesis of metallic nanoparticles is an auspicious method of preparing nanoparticles using plant extracts that have lesser toxicity to animal cells and the host. In the present work, we analyzed the antibacterial activity of <i>Citrullus colocynthis</i> and <i>Psidium guaja...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/9/2349 |
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| author | Ramsha Hafeez Zakia Kanwal Muhammad Akram Raza Shafqat Rasool Saira Riaz Shahzad Naseem Shifa Rabani Imran Haider Naushad Ahmad Suliman Yousef Alomar |
| author_facet | Ramsha Hafeez Zakia Kanwal Muhammad Akram Raza Shafqat Rasool Saira Riaz Shahzad Naseem Shifa Rabani Imran Haider Naushad Ahmad Suliman Yousef Alomar |
| author_sort | Ramsha Hafeez |
| collection | DOAJ |
| description | Green synthesis of metallic nanoparticles is an auspicious method of preparing nanoparticles using plant extracts that have lesser toxicity to animal cells and the host. In the present work, we analyzed the antibacterial activity of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i>-mediated silver nanoparticles (Cc-AgNPs and Pg-AgNPs, respectively) against <i>Aeromonas hydrophila</i> (<i>A. hydrophila</i>) in an in vivo assay employing <i>Labeo rohita</i> (<i>L. rohita</i>). <i>L. rohita</i> were divided into six groups for both Cc-AgNPs and Pg-AgNPs treatments separately: Control, <i>A. hydrophila</i> infected, <i>A. hydrophila</i> + Ampicillin, <i>A. hydrophila</i> + Cc/Pg-AgNPs (25 µg/L), <i>A. hydrophila</i> + Cc/Pg-AgNPs (50 µg/L), and <i>A. hydrophila</i> + Cc/Pg-AgNPs (75 µg/L). Changes in different bio-indicators such as hematological, histological, oxidative stress, and cytokine analysis were observed. Interestingly, the infected fish treated with both types of AgNPs (Cc-AgNPs and Pg-AgNPs) exhibited a higher survival rate than the untreated infected fish and demonstrated signs of recovery from the infection, providing a compelling indication of the positive impact of phytosynthesized AgNPs. Disruptions in hematological and histological parameters were found in the infected fish. Both Cc-AgNPs and Pg-AgNPs showed recovery on the hematological and histological parameters. Analysis of oxidative stress and cytokine markers also revealed provoking evidence of the positive impact of Cc-AgNPs and Pg-AgNPs treatment against disease progression in the infected fish. The major finding of the study was that the higher concentrations of the nanoparticles (50 µg/L in the case of Cc-AgNPs and 75 µg/L in the case of Pg-AgNPs) were more effective in fighting against disease. In conclusion, our work presents novel insights for the use of green-synthesized AgNPs as economic and innocuous antibacterial candidates in aquaculture. |
| first_indexed | 2024-03-10T23:01:18Z |
| format | Article |
| id | doaj.art-50dfc53d0de34d789b04cdab2b0a8c74 |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T23:01:18Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-50dfc53d0de34d789b04cdab2b0a8c742023-11-19T09:40:04ZengMDPI AGBiomedicines2227-90592023-08-01119234910.3390/biomedicines11092349Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i>Ramsha Hafeez0Zakia Kanwal1Muhammad Akram Raza2Shafqat Rasool3Saira Riaz4Shahzad Naseem5Shifa Rabani6Imran Haider7Naushad Ahmad8Suliman Yousef Alomar9Department of Zoology, Faculty of Natural Sciences, Lahore College for Women University, Jail Road, Lahore 54000, PakistanDepartment of Zoology, Faculty of Natural Sciences, Lahore College for Women University, Jail Road, Lahore 54000, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, Lahore 54590, PakistanDepartment of Zoology, Faculty of Natural Sciences, Lahore College for Women University, Jail Road, Lahore 54000, PakistanSwammerdam Institute for Life Sciences, University of Amsterdam, 1012 Amsterdam, The NetherlandsDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaGreen synthesis of metallic nanoparticles is an auspicious method of preparing nanoparticles using plant extracts that have lesser toxicity to animal cells and the host. In the present work, we analyzed the antibacterial activity of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i>-mediated silver nanoparticles (Cc-AgNPs and Pg-AgNPs, respectively) against <i>Aeromonas hydrophila</i> (<i>A. hydrophila</i>) in an in vivo assay employing <i>Labeo rohita</i> (<i>L. rohita</i>). <i>L. rohita</i> were divided into six groups for both Cc-AgNPs and Pg-AgNPs treatments separately: Control, <i>A. hydrophila</i> infected, <i>A. hydrophila</i> + Ampicillin, <i>A. hydrophila</i> + Cc/Pg-AgNPs (25 µg/L), <i>A. hydrophila</i> + Cc/Pg-AgNPs (50 µg/L), and <i>A. hydrophila</i> + Cc/Pg-AgNPs (75 µg/L). Changes in different bio-indicators such as hematological, histological, oxidative stress, and cytokine analysis were observed. Interestingly, the infected fish treated with both types of AgNPs (Cc-AgNPs and Pg-AgNPs) exhibited a higher survival rate than the untreated infected fish and demonstrated signs of recovery from the infection, providing a compelling indication of the positive impact of phytosynthesized AgNPs. Disruptions in hematological and histological parameters were found in the infected fish. Both Cc-AgNPs and Pg-AgNPs showed recovery on the hematological and histological parameters. Analysis of oxidative stress and cytokine markers also revealed provoking evidence of the positive impact of Cc-AgNPs and Pg-AgNPs treatment against disease progression in the infected fish. The major finding of the study was that the higher concentrations of the nanoparticles (50 µg/L in the case of Cc-AgNPs and 75 µg/L in the case of Pg-AgNPs) were more effective in fighting against disease. In conclusion, our work presents novel insights for the use of green-synthesized AgNPs as economic and innocuous antibacterial candidates in aquaculture.https://www.mdpi.com/2227-9059/11/9/2349phytosynthesissilver nanoparticles<i>Labeo rohita</i><i>Citrullus colocynthis</i><i>Psidium guajava</i>antibacterial activity |
| spellingShingle | Ramsha Hafeez Zakia Kanwal Muhammad Akram Raza Shafqat Rasool Saira Riaz Shahzad Naseem Shifa Rabani Imran Haider Naushad Ahmad Suliman Yousef Alomar Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i> Biomedicines phytosynthesis silver nanoparticles <i>Labeo rohita</i> <i>Citrullus colocynthis</i> <i>Psidium guajava</i> antibacterial activity |
| title | Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i> |
| title_full | Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i> |
| title_fullStr | Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i> |
| title_full_unstemmed | Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i> |
| title_short | Role of <i>Citrullus colocynthis</i> and <i>Psidium guajava</i> Mediated Green Synthesized Silver Nanoparticles in Disease Resistance against <i>Aeromonas hydrophila</i> Challenge in <i>Labeo rohita</i> |
| title_sort | role of i citrullus colocynthis i and i psidium guajava i mediated green synthesized silver nanoparticles in disease resistance against i aeromonas hydrophila i challenge in i labeo rohita i |
| topic | phytosynthesis silver nanoparticles <i>Labeo rohita</i> <i>Citrullus colocynthis</i> <i>Psidium guajava</i> antibacterial activity |
| url | https://www.mdpi.com/2227-9059/11/9/2349 |
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