Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria
Th e present study aimed at the direct detection of parasitic DNA in placenta samples of ewes by PCR targeting the B1 gene of Toxoplasma gondii. We identifi ed also the possible risk factors associated with the infection. A total of 307 female sheep from 23 farms were collected between 2019 and 2020...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2022-03-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2896 |
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| author | Nassima AIT ISSAD Khaled ABDELOUAHED Nora MIMOUNE Salim BEKHOUCHE Racha BOUBEUKER Haiet HAMOUDI ADJMI Thanina Ghania AIT HAMOUDA Djilali DEGUI Rachid KAIDI Djamel KHELEF |
| author_facet | Nassima AIT ISSAD Khaled ABDELOUAHED Nora MIMOUNE Salim BEKHOUCHE Racha BOUBEUKER Haiet HAMOUDI ADJMI Thanina Ghania AIT HAMOUDA Djilali DEGUI Rachid KAIDI Djamel KHELEF |
| author_sort | Nassima AIT ISSAD |
| collection | DOAJ |
| description | Th e present study aimed at the direct detection of parasitic DNA in placenta samples of ewes by PCR targeting the B1 gene of
Toxoplasma gondii. We identifi ed also the possible risk factors associated with the infection. A total of 307 female sheep from 23 farms were
collected between 2019 and 2020 in the Tebessa region in northeastern Algeria. Data showed that T. gondii DNA was detected in 114 of
the 307 tested females (37.1%). Th e on-farm molecular prevalence was 82.6%. High rates were revealed in Bir Al Ater (32.4%) and Tlidjen
(43.6%). Th e level of contamination was high in farms applying a sedentary mode (47.6%); this system tends to have a positive eff ect on
the prevalence of toxoplasmosis (P<0.05; 95% CI: 0.409-0.544). Moreover, it appears that the relationship between the presence of cats
on the farm and the prevalence of the disease was signifi cant (P<0.05; 95% CI: 0.445-0.597). In the same context, primiparous females
were 2.54 times more likely to be infected with T. gondii than multiparous animals (P=0.001). To conclude, the prevalence noticed in the
present study indicates a generalized exposure of sheep to T. gondii in the northeast of Algeria, which represents a major risk for animal
and public health. Th erefore, management measures should be implemented and improved in the farms of this region for better disease
control and eradication. |
| first_indexed | 2024-03-13T04:39:14Z |
| format | Article |
| id | doaj.art-50e14602097f495c9a4968c9739e6f5f |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:14Z |
| publishDate | 2022-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-50e14602097f495c9a4968c9739e6f5f2023-06-19T06:28:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512022-03-0128226727410.9775/kvfd.2021.268872896Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeriaNassima AIT ISSAD0Khaled ABDELOUAHED1Nora MIMOUNE2Salim BEKHOUCHE3Racha BOUBEUKER4Haiet HAMOUDI ADJMI5Thanina Ghania AIT HAMOUDA6Djilali DEGUI7Rachid KAIDI8Djamel KHELEF9IInstitute of Veterinary Sciences, Saad Dahleb University, BP270, Soumaa, 09000, Blida, ALGERIAArmy Central Hospital, Dr Mohamed Seghir Nekkache, Ain Naadja, BP244 (16208-Kouba), 16048 Djasr Kassentina, Algiers, ALGERIANHSVM, Issad Abbes, Oued Smar, Algiers, ALGERIAArmy Central Hospital, Dr Mohamed Seghir Nekkache, Ain Naadja, BP244 (16208-Kouba), 16048 Djasr Kassentina, Algiers, ALGERIAArmy Central Hospital, Dr Mohamed Seghir Nekkache, Ain Naadja, BP244 (16208-Kouba), 16048 Djasr Kassentina, Algiers, ALGERIAArmy Central Hospital, Dr Mohamed Seghir Nekkache, Ain Naadja, BP244 (16208-Kouba), 16048 Djasr Kassentina, Algiers, ALGERIAArmy Central Hospital, Dr Mohamed Seghir Nekkache, Ain Naadja, BP244 (16208-Kouba), 16048 Djasr Kassentina, Algiers, ALGERIALBRA Laboratory, Institute of Veterinary Sciences, Saad Dahleb University, Blida, ALGERIALBRA Laboratory, Institute of Veterinary Sciences, Saad Dahleb University, Blida, ALGERIANHSVM, Issad Abbes, Oued Smar, Algiers, ALGERIATh e present study aimed at the direct detection of parasitic DNA in placenta samples of ewes by PCR targeting the B1 gene of Toxoplasma gondii. We identifi ed also the possible risk factors associated with the infection. A total of 307 female sheep from 23 farms were collected between 2019 and 2020 in the Tebessa region in northeastern Algeria. Data showed that T. gondii DNA was detected in 114 of the 307 tested females (37.1%). Th e on-farm molecular prevalence was 82.6%. High rates were revealed in Bir Al Ater (32.4%) and Tlidjen (43.6%). Th e level of contamination was high in farms applying a sedentary mode (47.6%); this system tends to have a positive eff ect on the prevalence of toxoplasmosis (P<0.05; 95% CI: 0.409-0.544). Moreover, it appears that the relationship between the presence of cats on the farm and the prevalence of the disease was signifi cant (P<0.05; 95% CI: 0.445-0.597). In the same context, primiparous females were 2.54 times more likely to be infected with T. gondii than multiparous animals (P=0.001). To conclude, the prevalence noticed in the present study indicates a generalized exposure of sheep to T. gondii in the northeast of Algeria, which represents a major risk for animal and public health. Th erefore, management measures should be implemented and improved in the farms of this region for better disease control and eradication.https://vetdergikafkas.org/pdf.php?id=2896algeriaewesmolecular detectionplacentatoxoplasma gondii |
| spellingShingle | Nassima AIT ISSAD Khaled ABDELOUAHED Nora MIMOUNE Salim BEKHOUCHE Racha BOUBEUKER Haiet HAMOUDI ADJMI Thanina Ghania AIT HAMOUDA Djilali DEGUI Rachid KAIDI Djamel KHELEF Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria Kafkas Universitesi Veteriner Fakültesi Dergisi algeria ewes molecular detection placenta toxoplasma gondii |
| title | Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria |
| title_full | Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria |
| title_fullStr | Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria |
| title_full_unstemmed | Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria |
| title_short | Molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria |
| title_sort | molecular detection of toxoplasma gondii in ewes placenta in northeastern algeria |
| topic | algeria ewes molecular detection placenta toxoplasma gondii |
| url | https://vetdergikafkas.org/pdf.php?id=2896 |
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