Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer
Abstract Background Ovarian cancer typically is diagnosed late because insensitivity and lack of specificity of current biomarkers prior to its clinical detection. Ribosomal protein S6 (RPS6) is a ribosomal protein involved in the ribosomal 40S subunit, but its biological role in epithelial ovarian...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-08-01
|
| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-020-00707-7 |
| _version_ | 1797965408340279296 |
|---|---|
| author | Xueqing Yang Luxi Xu Yu-e Yang Chang Xiong Jinjin Yu Yuan Wang Yaying Lin |
| author_facet | Xueqing Yang Luxi Xu Yu-e Yang Chang Xiong Jinjin Yu Yuan Wang Yaying Lin |
| author_sort | Xueqing Yang |
| collection | DOAJ |
| description | Abstract Background Ovarian cancer typically is diagnosed late because insensitivity and lack of specificity of current biomarkers prior to its clinical detection. Ribosomal protein S6 (RPS6) is a ribosomal protein involved in the ribosomal 40S subunit, but its biological role in epithelial ovarian cancer (EOC) is still unknown. Results RPS6 was elevated in EOC compared to normal ovarian tissues and adenomas. Higher expression of RPS6 predicted worse prognosis in EOC. The level of RPS6 was correlated with clinical stage, histological type and pathological grade. Knockdown of RPS6 reduced the proliferation of ovarian cancer cell lines SKOV-3 and HO8910, and inhibit the migration and invasion ability. It revealed that cells arrested at G0G1 phase after knockdown of RPS6, and the expressions of CyclinD1, Cyclin E, CDK2, CDK4, CDK6 and pRb were also reduced. Conclusions RPS6 is involved in EOC and knockdown of RPS6 could inhibit the proliferation, invasion and migration ability of EOC in vitro by inducing G0/G1 phase arrest. RPS6 is expected to be a novel biomarker and molecular target to the EOC. |
| first_indexed | 2024-04-11T01:58:23Z |
| format | Article |
| id | doaj.art-50e31372fe2e496182ff6f618d326aa8 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T01:58:23Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-50e31372fe2e496182ff6f618d326aa82023-01-03T04:35:10ZengBMCJournal of Ovarian Research1757-22152020-08-0113111110.1186/s13048-020-00707-7Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancerXueqing Yang0Luxi Xu1Yu-e Yang2Chang Xiong3Jinjin Yu4Yuan Wang5Yaying Lin6Department of Medicine, Jiangnan UniversityDepartment of Medicine, Jiangnan UniversityDepartment of Medicine, Jiangnan UniversityDepartment of Medicine, Jiangnan UniversityDepartment of Obstetrics and Gynecology, Affiliated Hospital of Jiangnan UniversityDepartment of Obstetrics and Gynecology, Affiliated Hospital of Jiangnan UniversityDepartment of Obstetrics and Gynecology, Affiliated Hospital of Jiangnan UniversityAbstract Background Ovarian cancer typically is diagnosed late because insensitivity and lack of specificity of current biomarkers prior to its clinical detection. Ribosomal protein S6 (RPS6) is a ribosomal protein involved in the ribosomal 40S subunit, but its biological role in epithelial ovarian cancer (EOC) is still unknown. Results RPS6 was elevated in EOC compared to normal ovarian tissues and adenomas. Higher expression of RPS6 predicted worse prognosis in EOC. The level of RPS6 was correlated with clinical stage, histological type and pathological grade. Knockdown of RPS6 reduced the proliferation of ovarian cancer cell lines SKOV-3 and HO8910, and inhibit the migration and invasion ability. It revealed that cells arrested at G0G1 phase after knockdown of RPS6, and the expressions of CyclinD1, Cyclin E, CDK2, CDK4, CDK6 and pRb were also reduced. Conclusions RPS6 is involved in EOC and knockdown of RPS6 could inhibit the proliferation, invasion and migration ability of EOC in vitro by inducing G0/G1 phase arrest. RPS6 is expected to be a novel biomarker and molecular target to the EOC.http://link.springer.com/article/10.1186/s13048-020-00707-7Ribosomal protein S6 (RPS6)Epithelial ovarian cancer (EOC)G0G1 arrestCell proliferationInvasionMigration |
| spellingShingle | Xueqing Yang Luxi Xu Yu-e Yang Chang Xiong Jinjin Yu Yuan Wang Yaying Lin Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer Journal of Ovarian Research Ribosomal protein S6 (RPS6) Epithelial ovarian cancer (EOC) G0G1 arrest Cell proliferation Invasion Migration |
| title | Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer |
| title_full | Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer |
| title_fullStr | Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer |
| title_full_unstemmed | Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer |
| title_short | Knockdown of ribosomal protein S6 suppresses proliferation, migration, and invasion in epithelial ovarian cancer |
| title_sort | knockdown of ribosomal protein s6 suppresses proliferation migration and invasion in epithelial ovarian cancer |
| topic | Ribosomal protein S6 (RPS6) Epithelial ovarian cancer (EOC) G0G1 arrest Cell proliferation Invasion Migration |
| url | http://link.springer.com/article/10.1186/s13048-020-00707-7 |
| work_keys_str_mv | AT xueqingyang knockdownofribosomalproteins6suppressesproliferationmigrationandinvasioninepithelialovariancancer AT luxixu knockdownofribosomalproteins6suppressesproliferationmigrationandinvasioninepithelialovariancancer AT yueyang knockdownofribosomalproteins6suppressesproliferationmigrationandinvasioninepithelialovariancancer AT changxiong knockdownofribosomalproteins6suppressesproliferationmigrationandinvasioninepithelialovariancancer AT jinjinyu knockdownofribosomalproteins6suppressesproliferationmigrationandinvasioninepithelialovariancancer AT yuanwang knockdownofribosomalproteins6suppressesproliferationmigrationandinvasioninepithelialovariancancer AT yayinglin knockdownofribosomalproteins6suppressesproliferationmigrationandinvasioninepithelialovariancancer |