Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i>
The <i>Mi-1</i> gene in tomato confers resistance against insects and nematodes. The mechanisms involved in the functioning of this gene are not completely known, and they differ depending on the damaging organism (insect or nematode). Transcription factors (TF) from different families a...
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MDPI AG
2024-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/2/134 |
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| author | Susana Pascual Mariana Emiliozzi Gloria Nombela |
| author_facet | Susana Pascual Mariana Emiliozzi Gloria Nombela |
| author_sort | Susana Pascual |
| collection | DOAJ |
| description | The <i>Mi-1</i> gene in tomato confers resistance against insects and nematodes. The mechanisms involved in the functioning of this gene are not completely known, and they differ depending on the damaging organism (insect or nematode). Transcription factors (TF) from different families are essential for plant defence, and the TGAs, members of the Basic Leucine Zipper (bZIP) TF family, are relevant in different pathosystems. In this work, the implication of <i>TGA 1a</i> and <i>TGA 2.1</i> genes in <i>Mi-1</i> resistance against the root-knot nematode <i>Meloidogyne javanica</i> was studied, by virus-induced gene silencing (VIGS) based on Tobacco rattle virus (TRV). Results showed that infiltration with the empty TRV vector did not alter <i>Mi-1</i>-mediated resistance, confirming the adequacy of this method. Silencing of the <i>TGA 1a</i> gene resulted in a decrease in resistance to <i>M. javanica</i>, as the numbers of egg masses were significantly higher than those on non-silenced plants. This decrease in resistance was similar to that caused by silencing the <i>Mi-1</i> gene. However, the silencing of the <i>TGA 2.1</i> gene caused a limited loss of resistance, with infestation levels intermediate between those of resistant and susceptible varieties. Thus, our results demonstrate the requirement of <i>TGA 1a</i> in <i>Mi-1</i>-mediated resistance to <i>M. javanica</i>, while the incomplete silencing of <i>TGA 2.1</i> impaired a specific determination of its role. |
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| language | English |
| last_indexed | 2024-03-07T22:30:30Z |
| publishDate | 2024-01-01 |
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| series | Horticulturae |
| spelling | doaj.art-50e4d041b9e14c90bc52232506664e912024-02-23T15:18:41ZengMDPI AGHorticulturae2311-75242024-01-0110213410.3390/horticulturae10020134Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i>Susana Pascual0Mariana Emiliozzi1Gloria Nombela2Entomology Group, Plant Protection Department, INIA, CSIC, Ctra. Coruña km 7, 28040 Madrid, SpainDepartment of Plant Protection, Institute for Agricultural Sciences (ICA), Spanish National Research Council (CSIC), Serrano 115 Dpdo., 28006 Madrid, SpainDepartment of Plant Protection, Institute for Agricultural Sciences (ICA), Spanish National Research Council (CSIC), Serrano 115 Dpdo., 28006 Madrid, SpainThe <i>Mi-1</i> gene in tomato confers resistance against insects and nematodes. The mechanisms involved in the functioning of this gene are not completely known, and they differ depending on the damaging organism (insect or nematode). Transcription factors (TF) from different families are essential for plant defence, and the TGAs, members of the Basic Leucine Zipper (bZIP) TF family, are relevant in different pathosystems. In this work, the implication of <i>TGA 1a</i> and <i>TGA 2.1</i> genes in <i>Mi-1</i> resistance against the root-knot nematode <i>Meloidogyne javanica</i> was studied, by virus-induced gene silencing (VIGS) based on Tobacco rattle virus (TRV). Results showed that infiltration with the empty TRV vector did not alter <i>Mi-1</i>-mediated resistance, confirming the adequacy of this method. Silencing of the <i>TGA 1a</i> gene resulted in a decrease in resistance to <i>M. javanica</i>, as the numbers of egg masses were significantly higher than those on non-silenced plants. This decrease in resistance was similar to that caused by silencing the <i>Mi-1</i> gene. However, the silencing of the <i>TGA 2.1</i> gene caused a limited loss of resistance, with infestation levels intermediate between those of resistant and susceptible varieties. Thus, our results demonstrate the requirement of <i>TGA 1a</i> in <i>Mi-1</i>-mediated resistance to <i>M. javanica</i>, while the incomplete silencing of <i>TGA 2.1</i> impaired a specific determination of its role.https://www.mdpi.com/2311-7524/10/2/134nematodeshost resistancetomatoVIGS |
| spellingShingle | Susana Pascual Mariana Emiliozzi Gloria Nombela Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i> Horticulturae nematodes host resistance tomato VIGS |
| title | Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i> |
| title_full | Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i> |
| title_fullStr | Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i> |
| title_full_unstemmed | Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i> |
| title_short | Role of Two Transcription Factors (<i>TGA 1a</i> and <i>TGA 2.1</i>) in the <i>Mi-1</i>-Mediated Resistance of Tomato to the Root-Knot Nematode <i>Meloidogyne javanica</i> |
| title_sort | role of two transcription factors i tga 1a i and i tga 2 1 i in the i mi 1 i mediated resistance of tomato to the root knot nematode i meloidogyne javanica i |
| topic | nematodes host resistance tomato VIGS |
| url | https://www.mdpi.com/2311-7524/10/2/134 |
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