Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i>
The plant transcription factor <i>WRINKLED1</i> (<i>WRI1</i>), a member of <i>AP2/EREBP</i>, is involved in the regulation of glycolysis and the expression of genes related to the de novo synthesis of fatty acids in plastids. In this study, the key regulator of se...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/13/6996 |
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| author | Jing Sun Tian Chen Mi Liu Daqiu Zhao Jun Tao |
| author_facet | Jing Sun Tian Chen Mi Liu Daqiu Zhao Jun Tao |
| author_sort | Jing Sun |
| collection | DOAJ |
| description | The plant transcription factor <i>WRINKLED1</i> (<i>WRI1</i>), a member of <i>AP2/EREBP</i>, is involved in the regulation of glycolysis and the expression of genes related to the de novo synthesis of fatty acids in plastids. In this study, the key regulator of seed oil synthesis and accumulation transcription factor gene <i>PoWRI1</i> was identified and cloned, having a complete open reading frame of 1269 bp and encoding 422 amino acids. Subcellular localization analysis showed that <i>PoWRI1</i> is located at the nucleus. After the expression vector of <i>PoWRI1</i> was constructed and transformed into wild-type <i>Arabidopsis thaliana</i>, it was found that the overexpression of <i>PoWRI1</i> increased the expression level of downstream target genes such as <i>BCCP2</i>, <i>KAS1</i>, and <i>PKP-β1</i>. As a result, the seeds of transgenic plants became larger, the oil content increased significantly, and the unsaturated fatty acid content increased, which provide a scientific theoretical basis for the subsequent use of genetic engineering methods to improve the fatty acid composition and content of plant seeds. |
| first_indexed | 2024-03-09T04:46:49Z |
| format | Article |
| id | doaj.art-50e5f984210a4c67aec9efd0a038e7df |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:46:49Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-50e5f984210a4c67aec9efd0a038e7df2023-12-03T13:14:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213699610.3390/ijms22136996Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i>Jing Sun0Tian Chen1Mi Liu2Daqiu Zhao3Jun Tao4College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaThe plant transcription factor <i>WRINKLED1</i> (<i>WRI1</i>), a member of <i>AP2/EREBP</i>, is involved in the regulation of glycolysis and the expression of genes related to the de novo synthesis of fatty acids in plastids. In this study, the key regulator of seed oil synthesis and accumulation transcription factor gene <i>PoWRI1</i> was identified and cloned, having a complete open reading frame of 1269 bp and encoding 422 amino acids. Subcellular localization analysis showed that <i>PoWRI1</i> is located at the nucleus. After the expression vector of <i>PoWRI1</i> was constructed and transformed into wild-type <i>Arabidopsis thaliana</i>, it was found that the overexpression of <i>PoWRI1</i> increased the expression level of downstream target genes such as <i>BCCP2</i>, <i>KAS1</i>, and <i>PKP-β1</i>. As a result, the seeds of transgenic plants became larger, the oil content increased significantly, and the unsaturated fatty acid content increased, which provide a scientific theoretical basis for the subsequent use of genetic engineering methods to improve the fatty acid composition and content of plant seeds.https://www.mdpi.com/1422-0067/22/13/6996<i>Paeonia ostii</i><i>WRINKLED1</i>oil synthesis |
| spellingShingle | Jing Sun Tian Chen Mi Liu Daqiu Zhao Jun Tao Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i> International Journal of Molecular Sciences <i>Paeonia ostii</i> <i>WRINKLED1</i> oil synthesis |
| title | Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i> |
| title_full | Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i> |
| title_fullStr | Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i> |
| title_full_unstemmed | Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i> |
| title_short | Analysis and Functional Verification of <i>PoWRI1</i> Gene Associated with Oil Accumulation Process in <i>Paeonia ostii</i> |
| title_sort | analysis and functional verification of i powri1 i gene associated with oil accumulation process in i paeonia ostii i |
| topic | <i>Paeonia ostii</i> <i>WRINKLED1</i> oil synthesis |
| url | https://www.mdpi.com/1422-0067/22/13/6996 |
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