A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk method
Abstract Background A rising threat of the rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of public health concern worldwide. Hence, for a low income nation like Nepal, surveillance data on MBL producing clinical isolates via a cost effective...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-07-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-017-2640-7 |
| _version_ | 1819172929042120704 |
|---|---|
| author | Pratigya Thapa Dinesh Bhandari Dhiraj Shrestha Hiramani Parajuli Prakash Chaudhary Jyoti Amatya Ritu Amatya |
| author_facet | Pratigya Thapa Dinesh Bhandari Dhiraj Shrestha Hiramani Parajuli Prakash Chaudhary Jyoti Amatya Ritu Amatya |
| author_sort | Pratigya Thapa |
| collection | DOAJ |
| description | Abstract Background A rising threat of the rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of public health concern worldwide. Hence, for a low income nation like Nepal, surveillance data on MBL producing clinical isolates via a cost effective technique is necessary to prevent their dissemination as well as formulation and regulation of antimicrobial stewardship policy. Methods The prospective study was conducted at Nepal Medical College, Kathmandu from May to October, 2014 to assess the prevalence of MBL production among ceftazidime-resistant Gram-negative rods (GNRs) isolates. The samples were processed according to standard microbiological procedure following the Manual of clinical Microbiology. Isolated GNRs were subjected to susceptibility testing against the selected panel of antibiotics by Kirby- Bauer disc diffusion method and interpretation made in conformity with the Clinical and Laboratory Standards Institute (CLSI) guidelines. Ceftazidime-resistant isolates were subjected to the detection of MBL production by imipenem—EDTA combined disc (CD) method. Results Among the Gram-negative isolates, 5.80% (21/362) were found to be MBL positive with Acinetobacter spp. showing the highest prevalence i.e. 85.71% (18/21), followed by P. aeruginosa i.e. 14.29% (3/21). None of the other cefazidime resistant gram negative bacteria tested were found to be positive for MBL production with all the positive isolates determined to be Multidrug resistant (MDR) strains. Conclusion This study demonstrated a higher rate of resistance among P. aeruginosa and Acinetobacter spp. to a wide variety of antibiotic categories with an additional burden of MBL production within them, warranting a need for strict surveillance and rapid detection of MBL production among the GNRs. |
| first_indexed | 2024-12-22T20:14:59Z |
| format | Article |
| id | doaj.art-50e832af0ba44e31a38826484c63f12d |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-22T20:14:59Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-50e832af0ba44e31a38826484c63f12d2022-12-21T18:13:59ZengBMCBMC Research Notes1756-05002017-07-011011610.1186/s13104-017-2640-7A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk methodPratigya Thapa0Dinesh Bhandari1Dhiraj Shrestha2Hiramani Parajuli3Prakash Chaudhary4Jyoti Amatya5Ritu Amatya6Department of Microbiology, Trichandra Multiple CollegeDepartment of Microbiology, Trichandra Multiple CollegeDepartment of Microbiology, Trichandra Multiple CollegeDepartment of Microbiology, Trichandra Multiple CollegeDepartment of Microbiology, Trichandra Multiple CollegeDepartment of Microbiology, Trichandra Multiple CollegeDepartment of Microbiology, Nepal Medical College and Teaching Hospital, Kathmandu UniversityAbstract Background A rising threat of the rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of public health concern worldwide. Hence, for a low income nation like Nepal, surveillance data on MBL producing clinical isolates via a cost effective technique is necessary to prevent their dissemination as well as formulation and regulation of antimicrobial stewardship policy. Methods The prospective study was conducted at Nepal Medical College, Kathmandu from May to October, 2014 to assess the prevalence of MBL production among ceftazidime-resistant Gram-negative rods (GNRs) isolates. The samples were processed according to standard microbiological procedure following the Manual of clinical Microbiology. Isolated GNRs were subjected to susceptibility testing against the selected panel of antibiotics by Kirby- Bauer disc diffusion method and interpretation made in conformity with the Clinical and Laboratory Standards Institute (CLSI) guidelines. Ceftazidime-resistant isolates were subjected to the detection of MBL production by imipenem—EDTA combined disc (CD) method. Results Among the Gram-negative isolates, 5.80% (21/362) were found to be MBL positive with Acinetobacter spp. showing the highest prevalence i.e. 85.71% (18/21), followed by P. aeruginosa i.e. 14.29% (3/21). None of the other cefazidime resistant gram negative bacteria tested were found to be positive for MBL production with all the positive isolates determined to be Multidrug resistant (MDR) strains. Conclusion This study demonstrated a higher rate of resistance among P. aeruginosa and Acinetobacter spp. to a wide variety of antibiotic categories with an additional burden of MBL production within them, warranting a need for strict surveillance and rapid detection of MBL production among the GNRs.http://link.springer.com/article/10.1186/s13104-017-2640-7Metallo-beta-lactamaseSurveillanceCeftazidime-resistant Gram-negative rodsAntimicrobial resistance |
| spellingShingle | Pratigya Thapa Dinesh Bhandari Dhiraj Shrestha Hiramani Parajuli Prakash Chaudhary Jyoti Amatya Ritu Amatya A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk method BMC Research Notes Metallo-beta-lactamase Surveillance Ceftazidime-resistant Gram-negative rods Antimicrobial resistance |
| title | A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk method |
| title_full | A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk method |
| title_fullStr | A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk method |
| title_full_unstemmed | A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk method |
| title_short | A hospital based surveillance of metallo-beta-lactamase producing gram negative bacteria in Nepal by imipenem-EDTA disk method |
| title_sort | hospital based surveillance of metallo beta lactamase producing gram negative bacteria in nepal by imipenem edta disk method |
| topic | Metallo-beta-lactamase Surveillance Ceftazidime-resistant Gram-negative rods Antimicrobial resistance |
| url | http://link.springer.com/article/10.1186/s13104-017-2640-7 |
| work_keys_str_mv | AT pratigyathapa ahospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT dineshbhandari ahospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT dhirajshrestha ahospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT hiramaniparajuli ahospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT prakashchaudhary ahospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT jyotiamatya ahospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT rituamatya ahospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT pratigyathapa hospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT dineshbhandari hospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT dhirajshrestha hospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT hiramaniparajuli hospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT prakashchaudhary hospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT jyotiamatya hospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod AT rituamatya hospitalbasedsurveillanceofmetallobetalactamaseproducinggramnegativebacteriainnepalbyimipenemedtadiskmethod |