Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinoma
Breast cancer (BC) is the most common life-threatening malignancy amongst women with high incidence worldwide. In Egypt, it is the most known malignancy amongst females. Epithelial-mesenchymal transition (EMT) participates in breast tumors’ invasiveness, and metastasis, but the process is poorly und...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-08-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022014487 |
| _version_ | 1811188093017915392 |
|---|---|
| author | Fawziya A.E. Ragab Ibrahim Zain Ulabdeen Naser Hussein Amany I. Yousef Nadia Ahmed Abd El Moneim Amr Mahmoud Hussein Ayman Farouk Mohammad Ahmed Noha Mohamed Ragab O. Al-Masry |
| author_facet | Fawziya A.E. Ragab Ibrahim Zain Ulabdeen Naser Hussein Amany I. Yousef Nadia Ahmed Abd El Moneim Amr Mahmoud Hussein Ayman Farouk Mohammad Ahmed Noha Mohamed Ragab O. Al-Masry |
| author_sort | Fawziya A.E. Ragab Ibrahim |
| collection | DOAJ |
| description | Breast cancer (BC) is the most common life-threatening malignancy amongst women with high incidence worldwide. In Egypt, it is the most known malignancy amongst females. Epithelial-mesenchymal transition (EMT) participates in breast tumors’ invasiveness, and metastasis, but the process is poorly understood. The involvement of voltage-gated calcium channels signaling in EMT has not yet been fully explored. Therefore, the aim of this study was to investigate the possible role of T-type calcium channels in metastasis and EMT among breast cancer patients. The study was carried out on 48 female breast cancer patients who were divided into two groups; metastatic and non-metastatic. qRT-PCR was employed to measure the expression of EMT marker genes (N- cadherin, E-cadherin, Snail, Vimentin and T-type VGCCs genes (CACNA1G, CACNA1H, and CACNA1I). The results of the present study revealed differential expression of the EMT marker genes in blood and tissue of non-metastatic and metastatic breast cancer patients, with a clear tendency for the mesenchymal markers to be significantly elevated in metastatic patients as well as malignant tissues taken from non-metastatic patients as compared to their paired tumor adjacent normal (TAN) tissue. Both CACNA1H and CACNA1I (T-type VGCCs oncogenes) were significantly elevated in blood of metastatic patients when compared to non-metastatic ones. In contrast, CACNA1G (tumor suppressor) exhibited a significant decrease in metastatic patients. The strong correlation between the expression of T-type VGCCs and mesenchymal marker genes in metastatic breast cancer patients casts light on the role of T-type VGCCs in metastasis and their involved in tumor invasiveness. |
| first_indexed | 2024-04-11T14:13:26Z |
| format | Article |
| id | doaj.art-50ea07efd52a48b2b264a7988c7a94a1 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T14:13:26Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-50ea07efd52a48b2b264a7988c7a94a12022-12-22T04:19:37ZengElsevierHeliyon2405-84402022-08-0188e10160Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinomaFawziya A.E. Ragab Ibrahim0Zain Ulabdeen Naser Hussein1Amany I. Yousef2Nadia Ahmed Abd El Moneim3Amr Mahmoud Hussein4Ayman Farouk Mohammad Ahmed5Noha Mohamed Ragab6O. Al-Masry7Department of Applied Medical Chemistry, Medical Research Institute, University of Alexandria, Egypt; Corresponding author.Department of Applied Medical Chemistry, Medical Research Institute, University of Alexandria, EgyptDepartment of Applied Medical Chemistry, Medical Research Institute, University of Alexandria, EgyptDepartment of Cancer Management and Research, Medical Research Institute, University of Alexandria, EgyptDepartment of Cancer Management and Research, Medical Research Institute, University of Alexandria, EgyptDepartment of Experimental and Clinical Surgery, Medical Research Institute, University of Alexandria, EgyptDepartment of Pathology, Medical Research Institute, University of Alexandria, EgyptDepartment of Applied Medical Chemistry, Medical Research Institute, University of Alexandria, EgyptBreast cancer (BC) is the most common life-threatening malignancy amongst women with high incidence worldwide. In Egypt, it is the most known malignancy amongst females. Epithelial-mesenchymal transition (EMT) participates in breast tumors’ invasiveness, and metastasis, but the process is poorly understood. The involvement of voltage-gated calcium channels signaling in EMT has not yet been fully explored. Therefore, the aim of this study was to investigate the possible role of T-type calcium channels in metastasis and EMT among breast cancer patients. The study was carried out on 48 female breast cancer patients who were divided into two groups; metastatic and non-metastatic. qRT-PCR was employed to measure the expression of EMT marker genes (N- cadherin, E-cadherin, Snail, Vimentin and T-type VGCCs genes (CACNA1G, CACNA1H, and CACNA1I). The results of the present study revealed differential expression of the EMT marker genes in blood and tissue of non-metastatic and metastatic breast cancer patients, with a clear tendency for the mesenchymal markers to be significantly elevated in metastatic patients as well as malignant tissues taken from non-metastatic patients as compared to their paired tumor adjacent normal (TAN) tissue. Both CACNA1H and CACNA1I (T-type VGCCs oncogenes) were significantly elevated in blood of metastatic patients when compared to non-metastatic ones. In contrast, CACNA1G (tumor suppressor) exhibited a significant decrease in metastatic patients. The strong correlation between the expression of T-type VGCCs and mesenchymal marker genes in metastatic breast cancer patients casts light on the role of T-type VGCCs in metastasis and their involved in tumor invasiveness.http://www.sciencedirect.com/science/article/pii/S2405844022014487Breast cancerMetastasisEMTT-type VGCCsCACNA1HCACNA1I |
| spellingShingle | Fawziya A.E. Ragab Ibrahim Zain Ulabdeen Naser Hussein Amany I. Yousef Nadia Ahmed Abd El Moneim Amr Mahmoud Hussein Ayman Farouk Mohammad Ahmed Noha Mohamed Ragab O. Al-Masry Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinoma Heliyon Breast cancer Metastasis EMT T-type VGCCs CACNA1H CACNA1I |
| title | Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinoma |
| title_full | Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinoma |
| title_fullStr | Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinoma |
| title_full_unstemmed | Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinoma |
| title_short | Insights on possible interplay between epithelial-mesenchymal transition and T-type voltage gated calcium channels genes in metastatic breast carcinoma |
| title_sort | insights on possible interplay between epithelial mesenchymal transition and t type voltage gated calcium channels genes in metastatic breast carcinoma |
| topic | Breast cancer Metastasis EMT T-type VGCCs CACNA1H CACNA1I |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022014487 |
| work_keys_str_mv | AT fawziyaaeragabibrahim insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma AT zainulabdeennaserhussein insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma AT amanyiyousef insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma AT nadiaahmedabdelmoneim insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma AT amrmahmoudhussein insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma AT aymanfaroukmohammadahmed insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma AT nohamohamedragab insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma AT oalmasry insightsonpossibleinterplaybetweenepithelialmesenchymaltransitionandttypevoltagegatedcalciumchannelsgenesinmetastaticbreastcarcinoma |