Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection
Abstract The procedures currently used for hepatitis B (HB) detection are not suitable for screening, clinical diagnosis, and point-of-care testing (POCT). Therefore, we developed and tested a QCM-based immunosensor by surface modification with AuNP-PEIs to amplify the signal and provide an oriented...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-48766-2 |
| _version_ | 1797398007310712832 |
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| author | Zahra Saffari Reza Ahangari Cohan Mina Sepahi Mahdi Sadeqi Mehdi Khoobi Mojtaba Hamidi Fard Amir Ghavidel Fahimeh Bagheri Amiri Mohammad Reza Aghasadeghi Dariush Norouzian |
| author_facet | Zahra Saffari Reza Ahangari Cohan Mina Sepahi Mahdi Sadeqi Mehdi Khoobi Mojtaba Hamidi Fard Amir Ghavidel Fahimeh Bagheri Amiri Mohammad Reza Aghasadeghi Dariush Norouzian |
| author_sort | Zahra Saffari |
| collection | DOAJ |
| description | Abstract The procedures currently used for hepatitis B (HB) detection are not suitable for screening, clinical diagnosis, and point-of-care testing (POCT). Therefore, we developed and tested a QCM-based immunosensor by surface modification with AuNP-PEIs to amplify the signal and provide an oriented-immobilization surface. The AuNP-PEIs were characterized by ICP-Mass, UV/Vis, DLS, FE-SEM, and ATR-FTIR. After coating AuNP-PEIs on the gold electrode surface, anti-HBsAg antibodies were immobilized using NHS/EDC chemistry based on response surface methodology (RSM) optimization. The efficiency of the immunosensor was assessed by human sera and data were compared to gold-standard ELISA using receiver-operating-characteristic (ROC) analysis. FE-SEM, AFM, EDS, and EDS mapping confirmed AuNP-PEIs are homogeneously distributed on the surface with a high density and purity. After antibody immobilization, the immunosensor exhibited good recognition of HBsAg with a calibration curve of ∆F = − 6.910e-7x + 10(R2 = 0.9905), a LOD of 1.49 ng/mL, and a LOQ of 4.52 ng/mL. The immunosensor yielded reliable and accurate results with a specificity of 100% (95% CI 47.8–100.0) and sensitivity of 100% (95% CI 96.2–100.0). In conclusion, the fabricated immunosensor has the potential as an analytic tool with high sensitivity and specificity. However, further investigations are needed to convert it to a tiny lab-on-chip for HB diagnosis in clinical samples. |
| first_indexed | 2024-03-09T01:19:26Z |
| format | Article |
| id | doaj.art-50ecb4f543024baeb45236b3264c949d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T01:19:26Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-50ecb4f543024baeb45236b3264c949d2023-12-10T12:16:36ZengNature PortfolioScientific Reports2045-23222023-12-0113111510.1038/s41598-023-48766-2Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detectionZahra Saffari0Reza Ahangari Cohan1Mina Sepahi2Mahdi Sadeqi3Mehdi Khoobi4Mojtaba Hamidi Fard5Amir Ghavidel6Fahimeh Bagheri Amiri7Mohammad Reza Aghasadeghi8Dariush Norouzian9Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of IranNanobiotechnology Department, New Technologies Research Group, Pasteur Institute of IranNanobiotechnology Department, New Technologies Research Group, Pasteur Institute of IranApplied Biotechnology Research Center, Baqiyatallah University of Medical SciencesDepartment of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical SciencesHepatitis and AIDS Department, Pasteur Institute of IranPhysics Department, Sharif University of TechnologyDepartment of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of IranHepatitis and AIDS Department, Pasteur Institute of IranNanobiotechnology Department, New Technologies Research Group, Pasteur Institute of IranAbstract The procedures currently used for hepatitis B (HB) detection are not suitable for screening, clinical diagnosis, and point-of-care testing (POCT). Therefore, we developed and tested a QCM-based immunosensor by surface modification with AuNP-PEIs to amplify the signal and provide an oriented-immobilization surface. The AuNP-PEIs were characterized by ICP-Mass, UV/Vis, DLS, FE-SEM, and ATR-FTIR. After coating AuNP-PEIs on the gold electrode surface, anti-HBsAg antibodies were immobilized using NHS/EDC chemistry based on response surface methodology (RSM) optimization. The efficiency of the immunosensor was assessed by human sera and data were compared to gold-standard ELISA using receiver-operating-characteristic (ROC) analysis. FE-SEM, AFM, EDS, and EDS mapping confirmed AuNP-PEIs are homogeneously distributed on the surface with a high density and purity. After antibody immobilization, the immunosensor exhibited good recognition of HBsAg with a calibration curve of ∆F = − 6.910e-7x + 10(R2 = 0.9905), a LOD of 1.49 ng/mL, and a LOQ of 4.52 ng/mL. The immunosensor yielded reliable and accurate results with a specificity of 100% (95% CI 47.8–100.0) and sensitivity of 100% (95% CI 96.2–100.0). In conclusion, the fabricated immunosensor has the potential as an analytic tool with high sensitivity and specificity. However, further investigations are needed to convert it to a tiny lab-on-chip for HB diagnosis in clinical samples.https://doi.org/10.1038/s41598-023-48766-2 |
| spellingShingle | Zahra Saffari Reza Ahangari Cohan Mina Sepahi Mahdi Sadeqi Mehdi Khoobi Mojtaba Hamidi Fard Amir Ghavidel Fahimeh Bagheri Amiri Mohammad Reza Aghasadeghi Dariush Norouzian Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection Scientific Reports |
| title | Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection |
| title_full | Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection |
| title_fullStr | Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection |
| title_full_unstemmed | Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection |
| title_short | Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection |
| title_sort | signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles polyethyleneimine for hepatitis b biomarker detection |
| url | https://doi.org/10.1038/s41598-023-48766-2 |
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