Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor Signaling
Background: Long intergenic noncoding RNA regulator of reprogramming (linc-ROR) is a novel long noncoding RNA that exhibits significant effects on cancer progression. This research presented that linc-ROR had a crucial part in promoting biological characteristics associated with worse prognosis in c...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-08-01
|
| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338221114707 |
| _version_ | 1811308994237562880 |
|---|---|
| author | Ying Chen PhD Li Yang BA Dian Yin MM Xiu Feng Jing Jie Phd DengFu Yao PhD JianRong Chen PhD |
| author_facet | Ying Chen PhD Li Yang BA Dian Yin MM Xiu Feng Jing Jie Phd DengFu Yao PhD JianRong Chen PhD |
| author_sort | Ying Chen PhD |
| collection | DOAJ |
| description | Background: Long intergenic noncoding RNA regulator of reprogramming (linc-ROR) is a novel long noncoding RNA that exhibits significant effects on cancer progression. This research presented that linc-ROR had a crucial part in promoting biological characteristics associated with worse prognosis in colon cancer. Method: Bioinformatics analysis was performed to predict signaling pathways related to linc-ROR. In addition, western blot, quantitative reverse transcription-polymerase chain reaction, RNA-pulldown, cell proliferation assays, colony formation assays, wound healing assays, and transwell assays were applied to detect the role and regulation of particular molecules. Results: Our results showed that the knockdown of linc-ROR reduced cell invasion, proliferative ability, and migration in colon cancer. Further evaluation verified that downregulating linc-ROR inhibited the activation of epidermal growth factor receptor (EGFR) signaling. In addition, cbl-b, a kind of E3 ubiquitin ligase that increases the degradation of EGFR, was found to be a potential linc-ROR target. Conclusions: Based on our findings, it was presented that linc-ROR served a role as a tumor-promoting factor via repressing the ubiquitination and degradation of EGFR signaling, which indicated that it could be a possible prognostic marker and therapeutic target for colon cancer. |
| first_indexed | 2024-04-13T09:34:20Z |
| format | Article |
| id | doaj.art-50edba0095094b0e94c79175f90542ce |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-04-13T09:34:20Z |
| publishDate | 2022-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-50edba0095094b0e94c79175f90542ce2022-12-22T02:52:09ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382022-08-012110.1177/15330338221114707Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor SignalingYing Chen PhD0Li Yang BA1Dian Yin MM2Xiu Feng3Jing Jie Phd4DengFu Yao PhD5JianRong Chen PhD6 Department of Oncology, , Nantong, Jiangsu, China Department of Oncology, , Nantong, Jiangsu, China Department of Oncology, , Nantong, Jiangsu, China Department of Oncology, , Nantong, Jiangsu, China Department of Oncology, , Nantong, Jiangsu, China Research Center of Clinical Medicine, , Nantong, Jiangsu, China Department of Oncology, , Nantong, Jiangsu, ChinaBackground: Long intergenic noncoding RNA regulator of reprogramming (linc-ROR) is a novel long noncoding RNA that exhibits significant effects on cancer progression. This research presented that linc-ROR had a crucial part in promoting biological characteristics associated with worse prognosis in colon cancer. Method: Bioinformatics analysis was performed to predict signaling pathways related to linc-ROR. In addition, western blot, quantitative reverse transcription-polymerase chain reaction, RNA-pulldown, cell proliferation assays, colony formation assays, wound healing assays, and transwell assays were applied to detect the role and regulation of particular molecules. Results: Our results showed that the knockdown of linc-ROR reduced cell invasion, proliferative ability, and migration in colon cancer. Further evaluation verified that downregulating linc-ROR inhibited the activation of epidermal growth factor receptor (EGFR) signaling. In addition, cbl-b, a kind of E3 ubiquitin ligase that increases the degradation of EGFR, was found to be a potential linc-ROR target. Conclusions: Based on our findings, it was presented that linc-ROR served a role as a tumor-promoting factor via repressing the ubiquitination and degradation of EGFR signaling, which indicated that it could be a possible prognostic marker and therapeutic target for colon cancer.https://doi.org/10.1177/15330338221114707 |
| spellingShingle | Ying Chen PhD Li Yang BA Dian Yin MM Xiu Feng Jing Jie Phd DengFu Yao PhD JianRong Chen PhD Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor Signaling Technology in Cancer Research & Treatment |
| title | Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor Signaling |
| title_full | Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor Signaling |
| title_fullStr | Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor Signaling |
| title_full_unstemmed | Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor Signaling |
| title_short | Role of Long Noncoding RNA Regulator of Reprogramming in Colon Cancer Progression via Epidermal Growth Factor Receptor Signaling |
| title_sort | role of long noncoding rna regulator of reprogramming in colon cancer progression via epidermal growth factor receptor signaling |
| url | https://doi.org/10.1177/15330338221114707 |
| work_keys_str_mv | AT yingchenphd roleoflongnoncodingrnaregulatorofreprogrammingincoloncancerprogressionviaepidermalgrowthfactorreceptorsignaling AT liyangba roleoflongnoncodingrnaregulatorofreprogrammingincoloncancerprogressionviaepidermalgrowthfactorreceptorsignaling AT dianyinmm roleoflongnoncodingrnaregulatorofreprogrammingincoloncancerprogressionviaepidermalgrowthfactorreceptorsignaling AT xiufeng roleoflongnoncodingrnaregulatorofreprogrammingincoloncancerprogressionviaepidermalgrowthfactorreceptorsignaling AT jingjiephd roleoflongnoncodingrnaregulatorofreprogrammingincoloncancerprogressionviaepidermalgrowthfactorreceptorsignaling AT dengfuyaophd roleoflongnoncodingrnaregulatorofreprogrammingincoloncancerprogressionviaepidermalgrowthfactorreceptorsignaling AT jianrongchenphd roleoflongnoncodingrnaregulatorofreprogrammingincoloncancerprogressionviaepidermalgrowthfactorreceptorsignaling |