Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted Biomarkers
Currently available point‐of‐care systems for body fluid collection exhibit poor integration with sensors. Herein, the design of a disposable device for interstitial fluid (ISF) extraction as well as glucose, lactate, and potassium ion (K+) monitoring is reported on. It is minimally invasive and app...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-06-01
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| Series: | Small Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/smsc.202200087 |
| _version_ | 1797799283009781760 |
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| author | Shubhangi Shukla Sina Azizi Machekposhti Naveen Joshi Pratik Joshi Roger J. Narayan |
| author_facet | Shubhangi Shukla Sina Azizi Machekposhti Naveen Joshi Pratik Joshi Roger J. Narayan |
| author_sort | Shubhangi Shukla |
| collection | DOAJ |
| description | Currently available point‐of‐care systems for body fluid collection exhibit poor integration with sensors. Herein, the design of a disposable device for interstitial fluid (ISF) extraction as well as glucose, lactate, and potassium ion (K+) monitoring is reported on. It is minimally invasive and appropriate for single use, minimizing the risk of infection to the user. This microscale device contains a 3D‐printed cap‐like structure with a four‐by‐four microneedle (MN) array, bioreceptor‐modified carbon fiber (CF)‐sensing surface, and negative pressure convection technology. These features are incorporated within a compact, self‐contained, and manually operated microscale device, which is capable of withdrawing ≈3.0 μL of ISF from the skin. MN arrays applied with an upward driving force may increase the ISF flow rate. Moreover, functionalized CF working electrodes (WE1, WE2, WE3) are shown to selectively detect lactate, glucose, and K+ with high sensitivities of 0.258, 0.549, and 0.657 μA μm−1 cm−2 and low detection limits of 0.01, 0.080, 0.05 μm, respectively. Ex vivo testing on porcine skin is used to detect the ISF levels of the biomarkers. The microscale device can be a replacement for current point‐of‐care diagnostic approaches. |
| first_indexed | 2024-03-13T04:16:34Z |
| format | Article |
| id | doaj.art-50f143e8cb8349f6b135d8260b473cf9 |
| institution | Directory Open Access Journal |
| issn | 2688-4046 |
| language | English |
| last_indexed | 2024-03-13T04:16:34Z |
| publishDate | 2023-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Science |
| spelling | doaj.art-50f143e8cb8349f6b135d8260b473cf92023-06-21T00:42:44ZengWiley-VCHSmall Science2688-40462023-06-0136n/an/a10.1002/smsc.202200087Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted BiomarkersShubhangi Shukla0Sina Azizi Machekposhti1Naveen Joshi2Pratik Joshi3Roger J. Narayan4Joint Department of Biomedical Engineering North Carolina State University Raleigh NC 27695-7907 USAJoint Department of Biomedical Engineering North Carolina State University Raleigh NC 27695-7907 USADepartment of Materials Science and Engineering North Carolina State University Raleigh NC 27695-7907 USADepartment of Materials Science and Engineering North Carolina State University Raleigh NC 27695-7907 USAJoint Department of Biomedical Engineering North Carolina State University Raleigh NC 27695-7907 USACurrently available point‐of‐care systems for body fluid collection exhibit poor integration with sensors. Herein, the design of a disposable device for interstitial fluid (ISF) extraction as well as glucose, lactate, and potassium ion (K+) monitoring is reported on. It is minimally invasive and appropriate for single use, minimizing the risk of infection to the user. This microscale device contains a 3D‐printed cap‐like structure with a four‐by‐four microneedle (MN) array, bioreceptor‐modified carbon fiber (CF)‐sensing surface, and negative pressure convection technology. These features are incorporated within a compact, self‐contained, and manually operated microscale device, which is capable of withdrawing ≈3.0 μL of ISF from the skin. MN arrays applied with an upward driving force may increase the ISF flow rate. Moreover, functionalized CF working electrodes (WE1, WE2, WE3) are shown to selectively detect lactate, glucose, and K+ with high sensitivities of 0.258, 0.549, and 0.657 μA μm−1 cm−2 and low detection limits of 0.01, 0.080, 0.05 μm, respectively. Ex vivo testing on porcine skin is used to detect the ISF levels of the biomarkers. The microscale device can be a replacement for current point‐of‐care diagnostic approaches.https://doi.org/10.1002/smsc.2022000873D printingdiffusionglucose monitoringinterstitial fluidsmicroneedle arraystransdermal |
| spellingShingle | Shubhangi Shukla Sina Azizi Machekposhti Naveen Joshi Pratik Joshi Roger J. Narayan Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted Biomarkers Small Science 3D printing diffusion glucose monitoring interstitial fluids microneedle arrays transdermal |
| title | Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted Biomarkers |
| title_full | Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted Biomarkers |
| title_fullStr | Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted Biomarkers |
| title_full_unstemmed | Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted Biomarkers |
| title_short | Microneedle‐Integrated Device for Transdermal Sampling and Analyses of Targeted Biomarkers |
| title_sort | microneedle integrated device for transdermal sampling and analyses of targeted biomarkers |
| topic | 3D printing diffusion glucose monitoring interstitial fluids microneedle arrays transdermal |
| url | https://doi.org/10.1002/smsc.202200087 |
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