Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional Study
This study aimed to assess the qualitative changes in the saliva during the process of primary teeth eruption. This cross-sectional study was conducted on 147 children from 2 to 48 months, of which 49 were in group A (no erupted primary teeth), 53 were in group B (at least one active erupting primar...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/13/7/1335 |
| _version_ | 1797608176829333504 |
|---|---|
| author | Mina Ahmadian Sara Maleki Kambakhsh Nahid Einollahi Saber Babazadeh Maryam Tofangchiha Giuseppe D’Amato Romeo Patini |
| author_facet | Mina Ahmadian Sara Maleki Kambakhsh Nahid Einollahi Saber Babazadeh Maryam Tofangchiha Giuseppe D’Amato Romeo Patini |
| author_sort | Mina Ahmadian |
| collection | DOAJ |
| description | This study aimed to assess the qualitative changes in the saliva during the process of primary teeth eruption. This cross-sectional study was conducted on 147 children from 2 to 48 months, of which 49 were in group A (no erupted primary teeth), 53 were in group B (at least one active erupting primary tooth), and 45 were in group C (eruption of all 20 primary teeth was completed). Salivary proteins were evaluated by sodium dodecyl sulfate electrophoresis with polyacrylamide gel, while the concentrations of salivary sodium, potassium, chloride, and calcium ions were evaluated by ion selective electrodes. The data were analyzed using ANOVA and Bonferroni tests (alpha = 0.05). The concentration of proteins with molecular weights of 20–30 KDa was significantly higher in group A, and it gradually decreased with age. The concentration of proteins with molecular weights of 50–60 KDa in group B was significantly lower than those of groups A and C. The calcium ion concentration in group A was significantly higher than that of the other groups. The concentration of potassium ions was minimal in group C. The proteins and electrolyte profiles of the subjects’ saliva changed in the process of primary tooth eruption. The highest concentrations of proteins such as statherin, histatin, P-B peptide, and cystatin and the lowest concentrations of proteins such as amylase were present in group B. |
| first_indexed | 2024-03-11T05:39:45Z |
| format | Article |
| id | doaj.art-50f77df1ef52406687d6c0b942158ca4 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T05:39:45Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-50f77df1ef52406687d6c0b942158ca42023-11-17T16:31:09ZengMDPI AGDiagnostics2075-44182023-04-01137133510.3390/diagnostics13071335Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional StudyMina Ahmadian0Sara Maleki Kambakhsh1Nahid Einollahi2Saber Babazadeh3Maryam Tofangchiha4Giuseppe D’Amato5Romeo Patini6School of Dentistry, Qazvin University of Medical Sciences, Qazvin 34199-15315, IranSchool of Dentistry, Qazvin University of Medical Sciences, Qazvin 34199-15315, IranDepartment of Clinical Laboratory Sciences, Faculty of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran 14197-33141, IranDental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin 34199-15315, IranDental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin 34199-15315, IranUnicamillus International University of Health and Medical Sciences, 00131 Rome, ItalyDepartment of Head, Neck and Sense Organs, School of Dentistry, Catholic University of Sacred Heart, 00135 Rome, ItalyThis study aimed to assess the qualitative changes in the saliva during the process of primary teeth eruption. This cross-sectional study was conducted on 147 children from 2 to 48 months, of which 49 were in group A (no erupted primary teeth), 53 were in group B (at least one active erupting primary tooth), and 45 were in group C (eruption of all 20 primary teeth was completed). Salivary proteins were evaluated by sodium dodecyl sulfate electrophoresis with polyacrylamide gel, while the concentrations of salivary sodium, potassium, chloride, and calcium ions were evaluated by ion selective electrodes. The data were analyzed using ANOVA and Bonferroni tests (alpha = 0.05). The concentration of proteins with molecular weights of 20–30 KDa was significantly higher in group A, and it gradually decreased with age. The concentration of proteins with molecular weights of 50–60 KDa in group B was significantly lower than those of groups A and C. The calcium ion concentration in group A was significantly higher than that of the other groups. The concentration of potassium ions was minimal in group C. The proteins and electrolyte profiles of the subjects’ saliva changed in the process of primary tooth eruption. The highest concentrations of proteins such as statherin, histatin, P-B peptide, and cystatin and the lowest concentrations of proteins such as amylase were present in group B.https://www.mdpi.com/2075-4418/13/7/1335tooth eruptionsalivaproteinstoothdeciduoussodium |
| spellingShingle | Mina Ahmadian Sara Maleki Kambakhsh Nahid Einollahi Saber Babazadeh Maryam Tofangchiha Giuseppe D’Amato Romeo Patini Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional Study Diagnostics tooth eruption saliva proteins tooth deciduous sodium |
| title | Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional Study |
| title_full | Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional Study |
| title_fullStr | Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional Study |
| title_full_unstemmed | Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional Study |
| title_short | Salivary Protein and Electrolyte Profiles during Primary Teeth Eruption: A Cross-Sectional Study |
| title_sort | salivary protein and electrolyte profiles during primary teeth eruption a cross sectional study |
| topic | tooth eruption saliva proteins tooth deciduous sodium |
| url | https://www.mdpi.com/2075-4418/13/7/1335 |
| work_keys_str_mv | AT minaahmadian salivaryproteinandelectrolyteprofilesduringprimaryteetheruptionacrosssectionalstudy AT saramalekikambakhsh salivaryproteinandelectrolyteprofilesduringprimaryteetheruptionacrosssectionalstudy AT nahideinollahi salivaryproteinandelectrolyteprofilesduringprimaryteetheruptionacrosssectionalstudy AT saberbabazadeh salivaryproteinandelectrolyteprofilesduringprimaryteetheruptionacrosssectionalstudy AT maryamtofangchiha salivaryproteinandelectrolyteprofilesduringprimaryteetheruptionacrosssectionalstudy AT giuseppedamato salivaryproteinandelectrolyteprofilesduringprimaryteetheruptionacrosssectionalstudy AT romeopatini salivaryproteinandelectrolyteprofilesduringprimaryteetheruptionacrosssectionalstudy |