Development of a Sequential Injection Analysis System for the Determination of Saccharin
Saccharin is a powerfully sweet nonnutritive sweetener that has been approved for food-processing applications within the range of 100–1200 mg/kg. A simple, rapid, and cost-effective sequential injection analysis (SIA) technique was developed to determine the saccharin level. This method is based on...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/17/12/2891 |
| _version_ | 1798040458208739328 |
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| author | Budi Wibowotomo Jong-Bang Eun Jong Il Rhee |
| author_facet | Budi Wibowotomo Jong-Bang Eun Jong Il Rhee |
| author_sort | Budi Wibowotomo |
| collection | DOAJ |
| description | Saccharin is a powerfully sweet nonnutritive sweetener that has been approved for food-processing applications within the range of 100–1200 mg/kg. A simple, rapid, and cost-effective sequential injection analysis (SIA) technique was developed to determine the saccharin level. This method is based on the reaction of saccharin with p-chloranil in an ethanol medium with a hydrogen peroxide (H2O2) acceleration, and the resultant violet-red compound was detected using a UV-Vis spectrophotometer at λmax = 420 nm. To ascertain the optimal conditions for the SIA system, several parameters were investigated, including buffer flow rate and volume, p-chloranil concentration, and reactant volumes (saccharin, p-chloranil, and H2O2). The optimum setup of the SIA system was achieved with a buffer flow rate, buffer volume, and draw-up time of 1.2 mL/min, 2900 µL, and ~145 s, respectively. The optimal p-chloranil concentration is 30 mM, and the best reactant volumes, presented in an ordered sequence, are as follows: 30 µL of H2O2, 450 µL of saccharin, and 150 µL of p-chloranil. The optimized SIA configuration produced a good linear calibration curve with a correlation coefficient (R2 = 0.9812) in the concentration range of 20–140 mg/L and with a detection limit of 19.69 mg/L. Analytical applications in different food categories also showed acceptable recovery values in the range of 93.1–111.5%. This simple and rapid SIA system offers great feasibility for the saccharin quality control in food-product processing. |
| first_indexed | 2024-04-11T22:07:52Z |
| format | Article |
| id | doaj.art-50f7d4e0e19b47829ff41eb56c12b7b4 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:07:52Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-50f7d4e0e19b47829ff41eb56c12b7b42022-12-22T04:00:38ZengMDPI AGSensors1424-82202017-12-011712289110.3390/s17122891s17122891Development of a Sequential Injection Analysis System for the Determination of SaccharinBudi Wibowotomo0Jong-Bang Eun1Jong Il Rhee2Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, YongBong-Ro 77, Gwangju 61186, KoreaDepartment of Food Science and Technology and Functional Food Research Center, Chonnam National University, YongBong-Ro 77, Gwangju 61186, KoreaSchool of Chemical Engineering and Research Center for Biophotonics, Chonnam National University, YongBong-Ro 77, Gwangju 61186, KoreaSaccharin is a powerfully sweet nonnutritive sweetener that has been approved for food-processing applications within the range of 100–1200 mg/kg. A simple, rapid, and cost-effective sequential injection analysis (SIA) technique was developed to determine the saccharin level. This method is based on the reaction of saccharin with p-chloranil in an ethanol medium with a hydrogen peroxide (H2O2) acceleration, and the resultant violet-red compound was detected using a UV-Vis spectrophotometer at λmax = 420 nm. To ascertain the optimal conditions for the SIA system, several parameters were investigated, including buffer flow rate and volume, p-chloranil concentration, and reactant volumes (saccharin, p-chloranil, and H2O2). The optimum setup of the SIA system was achieved with a buffer flow rate, buffer volume, and draw-up time of 1.2 mL/min, 2900 µL, and ~145 s, respectively. The optimal p-chloranil concentration is 30 mM, and the best reactant volumes, presented in an ordered sequence, are as follows: 30 µL of H2O2, 450 µL of saccharin, and 150 µL of p-chloranil. The optimized SIA configuration produced a good linear calibration curve with a correlation coefficient (R2 = 0.9812) in the concentration range of 20–140 mg/L and with a detection limit of 19.69 mg/L. Analytical applications in different food categories also showed acceptable recovery values in the range of 93.1–111.5%. This simple and rapid SIA system offers great feasibility for the saccharin quality control in food-product processing.https://www.mdpi.com/1424-8220/17/12/2891p-chloranilfood-product processinghydrogen peroxidesaccharinsequential injection analysis |
| spellingShingle | Budi Wibowotomo Jong-Bang Eun Jong Il Rhee Development of a Sequential Injection Analysis System for the Determination of Saccharin Sensors p-chloranil food-product processing hydrogen peroxide saccharin sequential injection analysis |
| title | Development of a Sequential Injection Analysis System for the Determination of Saccharin |
| title_full | Development of a Sequential Injection Analysis System for the Determination of Saccharin |
| title_fullStr | Development of a Sequential Injection Analysis System for the Determination of Saccharin |
| title_full_unstemmed | Development of a Sequential Injection Analysis System for the Determination of Saccharin |
| title_short | Development of a Sequential Injection Analysis System for the Determination of Saccharin |
| title_sort | development of a sequential injection analysis system for the determination of saccharin |
| topic | p-chloranil food-product processing hydrogen peroxide saccharin sequential injection analysis |
| url | https://www.mdpi.com/1424-8220/17/12/2891 |
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