Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to Colistin
In recent years, the antimicrobial resistance in <i>Escherichia coli</i> has gradually developed into a global problem. These resistant bacteria could be transmitted to humans through animal feces in the environment or direct contact with pets, leading to a problem in bacterial treatment...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13428 |
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| author | Hui-Hua Zheng Chao Yu Xin-Yue Tang Chong-Tao Du Guang-Hong Xie |
| author_facet | Hui-Hua Zheng Chao Yu Xin-Yue Tang Chong-Tao Du Guang-Hong Xie |
| author_sort | Hui-Hua Zheng |
| collection | DOAJ |
| description | In recent years, the antimicrobial resistance in <i>Escherichia coli</i> has gradually developed into a global problem. These resistant bacteria could be transmitted to humans through animal feces in the environment or direct contact with pets, leading to a problem in bacterial treatment for humans and animals. Now, the antibiotic resistance of oral and intestinal microbiota from dog origins remains unclear in China. Therefore, this study first analyzed the current colistin resistance of oral and intestinal microbiota from dog origins in mainland China. A total of 536 samples were collected from dogs in mainland China and, respectively, cultured on the SS and MacConkey agar plate containing colistin (4 μg/mL) to obtain bacteria, and the antibiotic-resistance phenotype of <i>Escherichia coli</i> was investigated for nine antibiotics. Results showed that a total of <i>2259</i> colistin-resistant bacteria were isolated from samples and identified, and among them, the isolated rate of <i>Escherichia coli</i> (34.01%, 769/2259) was relatively higher than that of other bacteria. Subsequently, it was found that the resistance of these <i>Escherichia coli</i> was very severe by exploring its resistance to different antibiotics, particularly to three common antibiotics in a clinic which were ceftriaxone, ampicillin and trimethoprim/sulfamethoxazole, with the resistance rates of 60.60% (466/769), 57.22% (440/769), and 53.06% (408/769), respectively. Moreover, the simultaneous resistance of <i>Escherichia coli</i> to one or more antibiotics was determined, and 69.96% (538/769) strains have defined the resistance to both two or more antibiotics, and even 13 of <i>Escherichia coli</i> strains that were resistant to all nine antibiotics, indicating that the <i>Escherichia coli</i> from dog origins has severe antibiotic resistance in the clinic. In conclusion, this study guided the use of antibiotics and could draw attention to antibiotic resistance in veterinary clinical treatment for animals in the future. |
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| language | English |
| last_indexed | 2024-03-10T23:21:19Z |
| publishDate | 2023-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-50f93a53d4be466491772ed83a6fa2aa2023-11-19T08:17:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171342810.3390/ijms241713428Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to ColistinHui-Hua Zheng0Chao Yu1Xin-Yue Tang2Chong-Tao Du3Guang-Hong Xie4College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaIn recent years, the antimicrobial resistance in <i>Escherichia coli</i> has gradually developed into a global problem. These resistant bacteria could be transmitted to humans through animal feces in the environment or direct contact with pets, leading to a problem in bacterial treatment for humans and animals. Now, the antibiotic resistance of oral and intestinal microbiota from dog origins remains unclear in China. Therefore, this study first analyzed the current colistin resistance of oral and intestinal microbiota from dog origins in mainland China. A total of 536 samples were collected from dogs in mainland China and, respectively, cultured on the SS and MacConkey agar plate containing colistin (4 μg/mL) to obtain bacteria, and the antibiotic-resistance phenotype of <i>Escherichia coli</i> was investigated for nine antibiotics. Results showed that a total of <i>2259</i> colistin-resistant bacteria were isolated from samples and identified, and among them, the isolated rate of <i>Escherichia coli</i> (34.01%, 769/2259) was relatively higher than that of other bacteria. Subsequently, it was found that the resistance of these <i>Escherichia coli</i> was very severe by exploring its resistance to different antibiotics, particularly to three common antibiotics in a clinic which were ceftriaxone, ampicillin and trimethoprim/sulfamethoxazole, with the resistance rates of 60.60% (466/769), 57.22% (440/769), and 53.06% (408/769), respectively. Moreover, the simultaneous resistance of <i>Escherichia coli</i> to one or more antibiotics was determined, and 69.96% (538/769) strains have defined the resistance to both two or more antibiotics, and even 13 of <i>Escherichia coli</i> strains that were resistant to all nine antibiotics, indicating that the <i>Escherichia coli</i> from dog origins has severe antibiotic resistance in the clinic. In conclusion, this study guided the use of antibiotics and could draw attention to antibiotic resistance in veterinary clinical treatment for animals in the future.https://www.mdpi.com/1422-0067/24/17/13428dogcolistin resistance<i>Escherichia coli</i>antibiotic susceptibility testing |
| spellingShingle | Hui-Hua Zheng Chao Yu Xin-Yue Tang Chong-Tao Du Guang-Hong Xie Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to Colistin International Journal of Molecular Sciences dog colistin resistance <i>Escherichia coli</i> antibiotic susceptibility testing |
| title | Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to Colistin |
| title_full | Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to Colistin |
| title_fullStr | Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to Colistin |
| title_full_unstemmed | Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to Colistin |
| title_short | Isolation, Identification and Antimicrobial Resistance Analysis of Canine Oral and Intestinal <i>Escherichia coli</i> Resistant to Colistin |
| title_sort | isolation identification and antimicrobial resistance analysis of canine oral and intestinal i escherichia coli i resistant to colistin |
| topic | dog colistin resistance <i>Escherichia coli</i> antibiotic susceptibility testing |
| url | https://www.mdpi.com/1422-0067/24/17/13428 |
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