Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize loc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/6/1070 |
| _version_ | 1827690561099792384 |
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| author | Dan David Nick Storm Waksman Ilan Asaf Sol |
| author_facet | Dan David Nick Storm Waksman Ilan Asaf Sol |
| author_sort | Dan David |
| collection | DOAJ |
| description | Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017–2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel. |
| first_indexed | 2024-03-10T10:42:50Z |
| format | Article |
| id | doaj.art-50f98c759eef4d0290bd12601b35f78a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T10:42:50Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-50f98c759eef4d0290bd12601b35f78a2023-11-21T22:50:11ZengMDPI AGViruses1999-49152021-06-01136107010.3390/v13061070Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in IsraelDan David0Nick Storm1Waksman Ilan2Asaf Sol3Kimron Veterinary Institute, Bet Dagan 50250, IsraelKimron Veterinary Institute, Bet Dagan 50250, IsraelHachaklait, Veterinary Services, Caesarea 3079548, IsraelKimron Veterinary Institute, Bet Dagan 50250, IsraelBovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017–2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel.https://www.mdpi.com/1999-4915/13/6/1070bovine coronaviruswinter dysenteryspikeS1hemagglutinin esterase |
| spellingShingle | Dan David Nick Storm Waksman Ilan Asaf Sol Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel Viruses bovine coronavirus winter dysentery spike S1 hemagglutinin esterase |
| title | Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel |
| title_full | Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel |
| title_fullStr | Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel |
| title_full_unstemmed | Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel |
| title_short | Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel |
| title_sort | characterization of winter dysentery bovine coronavirus isolated from cattle in israel |
| topic | bovine coronavirus winter dysentery spike S1 hemagglutinin esterase |
| url | https://www.mdpi.com/1999-4915/13/6/1070 |
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