The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reacti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/5/430 |
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| author | Anja Sadžak Janez Mravljak Nadica Maltar-Strmečki Zoran Arsov Goran Baranović Ina Erceg Manfred Kriechbaum Vida Strasser Jan Přibyl Suzana Šegota |
| author_facet | Anja Sadžak Janez Mravljak Nadica Maltar-Strmečki Zoran Arsov Goran Baranović Ina Erceg Manfred Kriechbaum Vida Strasser Jan Přibyl Suzana Šegota |
| author_sort | Anja Sadžak |
| collection | DOAJ |
| description | The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury. |
| first_indexed | 2024-03-10T19:47:33Z |
| format | Article |
| id | doaj.art-50f9b46a8897407c9cd48b15cc96b4ad |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T19:47:33Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-50f9b46a8897407c9cd48b15cc96b4ad2023-11-20T00:38:51ZengMDPI AGAntioxidants2076-39212020-05-019543010.3390/antiox9050430The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid PeroxidationAnja Sadžak0Janez Mravljak1Nadica Maltar-Strmečki2Zoran Arsov3Goran Baranović4Ina Erceg5Manfred Kriechbaum6Vida Strasser7Jan Přibyl8Suzana Šegota9Ruđer Bošković Institute, 10000 Zagreb, CroatiaFaculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, SloveniaRuđer Bošković Institute, 10000 Zagreb, CroatiaJožef Stefan Institute, 1000 Ljubljana, SloveniaRuđer Bošković Institute, 10000 Zagreb, CroatiaRuđer Bošković Institute, 10000 Zagreb, CroatiaInstitute of Inorganic Chemistry, Graz University of Technology, 8010 Graz, AustriaRuđer Bošković Institute, 10000 Zagreb, CroatiaCEITEC, Masaryk University, 62500 Brno, Czech RepublicRuđer Bošković Institute, 10000 Zagreb, CroatiaThe structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.https://www.mdpi.com/2076-3921/9/5/430bilayer thicknesselasticityflavonolsfluiditylipid peroxidationmyricetin |
| spellingShingle | Anja Sadžak Janez Mravljak Nadica Maltar-Strmečki Zoran Arsov Goran Baranović Ina Erceg Manfred Kriechbaum Vida Strasser Jan Přibyl Suzana Šegota The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation Antioxidants bilayer thickness elasticity flavonols fluidity lipid peroxidation myricetin |
| title | The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation |
| title_full | The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation |
| title_fullStr | The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation |
| title_full_unstemmed | The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation |
| title_short | The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation |
| title_sort | structural integrity of the model lipid membrane during induced lipid peroxidation the role of flavonols in the inhibition of lipid peroxidation |
| topic | bilayer thickness elasticity flavonols fluidity lipid peroxidation myricetin |
| url | https://www.mdpi.com/2076-3921/9/5/430 |
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