Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh
Background and Aim: Salmonella spp. are frequently associated with various parts of the egg, including the shell, and cause foodborne outbreaks worldwide. Antibiotic-resistant Salmonella spp. pose serious threats to human and animal health; therefore, preventive measures against these pathogens are...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-11-01
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| Series: | International Journal of One Health |
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| Online Access: | https://www.onehealthjournal.org/Vol.8/No.2/4.pdf |
| _version_ | 1828094800303226880 |
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| author | Talal Hossain Md. Abu Sayem Khan Md. Firoz Ahmed Sabita Rezwana Rahman |
| author_facet | Talal Hossain Md. Abu Sayem Khan Md. Firoz Ahmed Sabita Rezwana Rahman |
| author_sort | Talal Hossain |
| collection | DOAJ |
| description | Background and Aim: Salmonella spp. are frequently associated with various parts of the egg, including the shell, and cause foodborne outbreaks worldwide. Antibiotic-resistant Salmonella spp. pose serious threats to human and animal health; therefore, preventive measures against these pathogens are important. This study aimed to isolate and characterize Salmonella spp. from eggshell samples from different local markets in Dhaka, Bangladesh.
Materials and Methods: Salmonella spp. were recovered from eggshells by enrichment culture and biochemical tests and characterized through molecular amplification of Salmonella-specific genes. Antibiotic sensitivity testing and molecular detection of isolates were performed by disk diffusion method and polymerase chain reaction (PCR), respectively. The invA, fliC, and sdfI genes were used in PCR to identify the genus Salmonella, and the species Salmonella Typhimurium and Salmonella Enteritidis, respectively.
Results: The prevalence of Salmonella spp. was recorded as 40%, in which S. Typhimurium was the predominant serotype. PCR analysis revealed that 100%, 59%, and 13.6% of these isolates possessed the invA, fliC, and sdfI genes, respectively. The isolates exhibited multidrug resistance phenotypes, with resistance (95.5%) toward tetracycline, sulfamethoxazole, and clindamycin and sensitivity (86.3%) toward chloramphenicol.
Conclusion: The findings of this study reflect the potential of eggs as a reservoir of multidrug-resistant Salmonella spp.; therefore, we recommend the careful handling of eggs to avoid contamination from farm to market. |
| first_indexed | 2024-04-11T07:04:19Z |
| format | Article |
| id | doaj.art-51014b4f96dd449fb0aad2a35911eeb5 |
| institution | Directory Open Access Journal |
| issn | 2455-5673 2455-8931 |
| language | English |
| last_indexed | 2024-04-11T07:04:19Z |
| publishDate | 2022-11-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | International Journal of One Health |
| spelling | doaj.art-51014b4f96dd449fb0aad2a35911eeb52022-12-22T04:38:26ZengVeterinary WorldInternational Journal of One Health2455-56732455-89312022-11-018210110710.14202/IJOH.2022.101-107Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, BangladeshTalal Hossain0https://orcid.org/0000-0002-0081-2098Md. Abu Sayem Khan1https://orcid.org/0000-0001-5393-8017Md. Firoz Ahmed2https://orcid.org/0000-0002-6218-201XSabita Rezwana Rahman3https://orcid.org/0000-0001-8428-6998Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.Department of Microbiology, Jahangirnagar University, Savar, Dhaka, Bangladesh.Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.Background and Aim: Salmonella spp. are frequently associated with various parts of the egg, including the shell, and cause foodborne outbreaks worldwide. Antibiotic-resistant Salmonella spp. pose serious threats to human and animal health; therefore, preventive measures against these pathogens are important. This study aimed to isolate and characterize Salmonella spp. from eggshell samples from different local markets in Dhaka, Bangladesh. Materials and Methods: Salmonella spp. were recovered from eggshells by enrichment culture and biochemical tests and characterized through molecular amplification of Salmonella-specific genes. Antibiotic sensitivity testing and molecular detection of isolates were performed by disk diffusion method and polymerase chain reaction (PCR), respectively. The invA, fliC, and sdfI genes were used in PCR to identify the genus Salmonella, and the species Salmonella Typhimurium and Salmonella Enteritidis, respectively. Results: The prevalence of Salmonella spp. was recorded as 40%, in which S. Typhimurium was the predominant serotype. PCR analysis revealed that 100%, 59%, and 13.6% of these isolates possessed the invA, fliC, and sdfI genes, respectively. The isolates exhibited multidrug resistance phenotypes, with resistance (95.5%) toward tetracycline, sulfamethoxazole, and clindamycin and sensitivity (86.3%) toward chloramphenicol. Conclusion: The findings of this study reflect the potential of eggs as a reservoir of multidrug-resistant Salmonella spp.; therefore, we recommend the careful handling of eggs to avoid contamination from farm to market.https://www.onehealthjournal.org/Vol.8/No.2/4.pdfantibiotic resistanceeggshealth hazardspoultry industrysalmonella |
| spellingShingle | Talal Hossain Md. Abu Sayem Khan Md. Firoz Ahmed Sabita Rezwana Rahman Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh International Journal of One Health antibiotic resistance eggs health hazards poultry industry salmonella |
| title | Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh |
| title_full | Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh |
| title_fullStr | Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh |
| title_full_unstemmed | Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh |
| title_short | Prevalence and molecular detection of multidrug-resistant Salmonella spp. isolated from eggshells in the local markets of Dhaka, Bangladesh |
| title_sort | prevalence and molecular detection of multidrug resistant salmonella spp isolated from eggshells in the local markets of dhaka bangladesh |
| topic | antibiotic resistance eggs health hazards poultry industry salmonella |
| url | https://www.onehealthjournal.org/Vol.8/No.2/4.pdf |
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