Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms

Back ground: Leuconostoc is one of the species of lactic acid bacteria that produced biofilms.Probiotic bacteria that produced biofilm  has  been used as naturopathy  against different microbial pathogens. Objective: This study was conducted to determine the antimicrobial activity of  Leuconosto...

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Main Authors: Asmaa S. Ahmed, abdullah Awad
Format: Article
Language:English
Published: Al-Nahrain University/ Biotechnology Research Center 2019-01-01
Series:مجلة مركز بحوث التقنيات الاحيائية
Subjects:
Online Access:https://www.jobrc.org/index.php/jobrc/article/view/570
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author Asmaa S. Ahmed
abdullah Awad
author_facet Asmaa S. Ahmed
abdullah Awad
author_sort Asmaa S. Ahmed
collection DOAJ
description Back ground: Leuconostoc is one of the species of lactic acid bacteria that produced biofilms.Probiotic bacteria that produced biofilm  has  been used as naturopathy  against different microbial pathogens. Objective: This study was conducted to determine the antimicrobial activity of  Leuconostoc  biofilm , against 24 isolates (4 of 6 different types) of food borne pathogens including Staphylococcus aureus , Salmonella spp, Escherichia coli , Klebsiella spp , Pseudomonas aeruginosa , Streptococcus mutans , Bacillus subtillus , Bacillus cereus, Bacillus sterothermophillus and Candida albicans. Materials and methods: using various concentration in vitro by filter paper disk diffusion method . Result: The present study showed the potent antimicrobial activity of the Leuconostoc mesenteroides biofilm against the all tested bacterial pathogens except  Bacillus species and yeast Candida albicans. Biofilm produced by Leuconostoc mesenteroides  showed highest zone of inhibition (13mm) against  Escherichia coli  and lowest zone of inhibition (7.0mm) against Streptococcus mutans . Conclusion: Consequently, Leuconostoc mesenteroides biofilm may be used as an antimicrobial agent in food products to prevent spoilage.
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spelling doaj.art-5105bce947654df9b73eb713f6561d772024-08-30T07:53:47ZengAl-Nahrain University/ Biotechnology Research Centerمجلة مركز بحوث التقنيات الاحيائية1815-11402708-13702019-01-0113110.24126/jobrc.2019.13.1.570Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganismsAsmaa S. Ahmedabdullah Awad Back ground: Leuconostoc is one of the species of lactic acid bacteria that produced biofilms.Probiotic bacteria that produced biofilm  has  been used as naturopathy  against different microbial pathogens. Objective: This study was conducted to determine the antimicrobial activity of  Leuconostoc  biofilm , against 24 isolates (4 of 6 different types) of food borne pathogens including Staphylococcus aureus , Salmonella spp, Escherichia coli , Klebsiella spp , Pseudomonas aeruginosa , Streptococcus mutans , Bacillus subtillus , Bacillus cereus, Bacillus sterothermophillus and Candida albicans. Materials and methods: using various concentration in vitro by filter paper disk diffusion method . Result: The present study showed the potent antimicrobial activity of the Leuconostoc mesenteroides biofilm against the all tested bacterial pathogens except  Bacillus species and yeast Candida albicans. Biofilm produced by Leuconostoc mesenteroides  showed highest zone of inhibition (13mm) against  Escherichia coli  and lowest zone of inhibition (7.0mm) against Streptococcus mutans . Conclusion: Consequently, Leuconostoc mesenteroides biofilm may be used as an antimicrobial agent in food products to prevent spoilage. https://www.jobrc.org/index.php/jobrc/article/view/570Biofilm, Dextran, Exopolysaccharide, Leuconostoc mesenteroides, Antimicrobial activity
spellingShingle Asmaa S. Ahmed
abdullah Awad
Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms
مجلة مركز بحوث التقنيات الاحيائية
Biofilm, Dextran, Exopolysaccharide, Leuconostoc mesenteroides, Antimicrobial activity
title Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms
title_full Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms
title_fullStr Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms
title_full_unstemmed Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms
title_short Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms
title_sort antimicrobial activity of leuconostoc mesenteroides biofilm against different microorganisms
topic Biofilm, Dextran, Exopolysaccharide, Leuconostoc mesenteroides, Antimicrobial activity
url https://www.jobrc.org/index.php/jobrc/article/view/570
work_keys_str_mv AT asmaasahmed antimicrobialactivityofleuconostocmesenteroidesbiofilmagainstdifferentmicroorganisms
AT abdullahawad antimicrobialactivityofleuconostocmesenteroidesbiofilmagainstdifferentmicroorganisms